Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,357,2020CT95.2% 59.9 / ‑3.7 22intergenic (‑92/+221)sapA/ymjAputative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (15/5);  total (16/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.93e-01

GACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCAT  >  NC_000913/1356991‑1357430
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    
gACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTg                                                                                                                                                                                                 <  1:190555/249‑1 (MQ=255)
   gCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTACCTGACCACTCACAAGTCCAGCACTCACCAAAAGAGACGATCATACCTGGCGCATAATATTCAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATTATGGCTGTGTGTTTGATCGTTGAACATGTGACGTCGCCATCTGTTcc                                                                                                                                                                                             >  2:70174/1‑249 (MQ=255)
                  gCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGa                                                                                                                                                                             <  1:146129/251‑1 (MQ=255)
                                            gagaTTCAGGCGCGGCGAATGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGGGATCAGGGGTCGTAATGAGGGATGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGa                                                                                                                                                    >  2:227790/1‑250 (MQ=255)
                                               aTTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGaaca                                                                                                                                                 >  2:177777/1‑250 (MQ=255)
                                                         ggcgATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCgagg                                                                                                                                      >  1:248601/1‑251 (MQ=255)
                                                                           cacaAGTCCAACAATCGCCAAAAAAGACGCAAATGCCTGGCGCATAATATGAAGGGATATTAAGTAAAGAGGCTATTATACTAATATTTAATGGTATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCAGTCTGGGTGGTCGTTGAACAGGTGACTTCGCCATCTGTTCAACATTCGTGCCCGAGCATTCTCTATAACTATAACTCATAGAAGATCTTAGCAAGGAGCAGAGCGGTACGATc                                                                                                                     <  2:54980/250‑1 (MQ=255)
                                                                                              aaaaGAGACGATAATAACTGGCGAATAATATTAAGGGATTTTAGGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTTCCCGTGATTTCTCTATAACTATAACTCTCAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCa                                                                                                 <  1:197270/251‑1 (MQ=255)
                                                                                                                    gcATAATATTAAGGGTTTTTATGAAACGAGGCTATCTTAATAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACCTTCGTACACGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGACACCCATTAAAGCACGCTGCCGTGCGTGTCATTGtt                                                                           >  2:240390/1‑251 (MQ=255)
                                                                                                                             tAAGGGAATTTATGTAAGGAGGCTATCTTAATAATCTTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGAGACGTCGACATCTGTTCAACATTCGTACCCGGGTTTTCTCTATAACTATAACTCACAGAACAACTTCGCGAGGAGCAGAGCGGTACAAACATGAGAAACCAATTAAAGCACACGGAGGTGCGCGTCATGTTTAGCCAGATg                                                                  >  2:135800/1‑251 (MQ=255)
                                                                                                                                   aTTTTATGTAAAGAGGCTATCTAACTAATATTTAATGACATTTGCCTTTACAGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTATATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGAACGAACGTGAGAAACCCATTAAAGAACGCTGCAGAGCGTGGCATTGTTAGCCGGATgcggcgg                                                            >  2:39993/1‑250 (MQ=255)
                                                                                                                                            aaaGAGGCTATCTTACTAATATCTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCttt                                                   >  1:314663/1‑251 (MQ=255)
                                                                                                                                                agGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATcc                                               >  1:266018/1‑251 (MQ=255)
                                                                                                                                                    tATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGAc                                           >  1:122335/1‑251 (MQ=255)
                                                                                                                                                       cTTACTAATATTTAATGACATTTGCCATTACAGTTTGTGATCAGGGGTCGTACTGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGCCGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACACATTAGCGAGGCACAGAGCGGTACGATCGTGAGAAACCCCTTAAAGCACGCCTCAGTGCGTGTAATTGTTAGCCAGATGCGGAGTGAACGCTTTATCCGGACAAc                                        >  2:337766/1‑251 (MQ=255)
                                                                                                                                                           cTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTgag                                                                                                                >  1:357152/1‑175 (MQ=255)
                                                                                                                                                           cTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTgag                                                                                                                <  2:357152/175‑1 (MQ=255)
                                                                                                                                                           cTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAAAGAt                                     >  1:21385/1‑250 (MQ=255)
                                                                                                                                                                    aaTGACATTTGCCATTACCGTTTGTGGTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGAt                           <  2:122335/251‑1 (MQ=255)
                                                                                                                                                                          aTTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTg                     >  1:88171/1‑251 (MQ=255)
                                                                                                                                                                                     ccGTTTGTGTTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtc           >  1:345861/1‑250 (MQ=255)
                                                                                                                                                                                             gtTCAGGGGTCGTAATGAGGGCTGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGCGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGGGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAAtcattactcat  >  1:70657/1‑251 (MQ=255)
                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    
GACGCAATAGACAAAACCGCTGTCGCGGATATCAGCATGCGGGGGAGATTCAGGCGCGGCGATTGCCTGACCACTCACAAGTCCAGCAATCACCAAAAGAGACGATAATACCTGGCGCATAATATTAAGGGATTTTATGTAAAGAGGCTATCTTACTAATATTTAATGACATTTGCCATTACCGTTTGTGTTCAGGGGTCGTAATGAGGGCCGTGTGGTTGGTCGTTGAACAGGTGACGTCGCCATCTGTTCAACATTCGTACCCGTGATTTCTCTATAACTATAACTCACAGAACAACTTAGCGAGGAGCAGAGCGGTACGATCGTGAGAAACCCATTAAAGCACGCTGCAGTGCGTGTCATTGTTAGCCAGATGCGGCGTGAACGCTTTATCCGGACAACGATACTGACCGATCGTCTGCAATTAAATCATTACTCAT  >  NC_000913/1356991‑1357430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑