Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 244,775 G→A G150D (GGC→GAC)  yafJ → putative glutamine amidotransferase YafJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913244,7750GA97.4% 116.5 / ‑4.6 38G150D (GGC→GAC) yafJputative glutamine amidotransferase YafJ
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (17/20);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

CGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACC‑GAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGAC  >  NC_000913/244529‑245021
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
cGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAAc                                                                                                                                                                                                                                                       <  1:88649/251‑1 (MQ=255)
 gATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGacaaca                                                                                                                                                                                                                                                      <  1:335538/251‑1 (MQ=255)
   aaaaaTCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATg                                                                                                                                                                                                                                                    <  2:175209/250‑1 (MQ=255)
      aacccTGTTCGGGGGTGGCTCAGATTCACAAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTACCCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTAGGCCCATAACGGACACCTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAACTAACGCAGCGTTACCCGCGCACACCGGACAACATggcg                                                                                                                                                                                                                                                 <  2:67981/248‑1 (MQ=255)
       aTCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATggcgg                                                                                                                                                                                                                                                >  1:188889/1‑251 (MQ=255)
         ccTGTACGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATCCTCACCCATTTACCCGCGAGTTATGGGGGCGGAACTGGACTTATGCCCATCACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCGCCTGCATAAATTAACGCAGCGGTACCCGCGCACACCGGACAACTTggcggc                                                                                                                                                                                                                                               <  2:302101/250‑1 (MQ=255)
                    gtggCTCATATTCGCCAGGCTAATCGGGGCGCGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAat                                                                                                                                                                                                                                    >  2:349548/1‑250 (MQ=255)
                     tggCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAatat                                                                                                                                                                                                                                  >  2:184537/1‑251 (MQ=255)
                               tCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCa                                                                                                                                                                                                                         <  1:50418/250‑1 (MQ=255)
                                    aGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCg                                                                                                                                                                                                                   <  1:230010/251‑1 (MQ=255)
                                                   aGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCCCTGGccgggggaatggtgcg                                                                                                                                                                                                     >  2:160923/1‑235 (MQ=255)
                                                             ggAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGtt                                                                                                                                                                                           <  1:348196/250‑1 (MQ=255)
                                                             ggAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATAAACTGCGGCAGAAGGGCggtt                                                                                                                                                                                          >  1:119012/1‑248 (MQ=255)
                                                                       cACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACagg                                                                                                                                                                                  <  1:77587/249‑3 (MQ=255)
                                                                          ccATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATgctgct                                                                                                                                                                             <  1:351675/251‑1 (MQ=255)
                                                                             tttACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTc                                                                                                                                                                          >  1:72721/1‑251 (MQ=255)
                                                                                  ccGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACg                                                                                                                                                                     <  2:3774/251‑1 (MQ=255)
                                                                                   cgcgAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGGACTGCGGCAGAAGGGCGGTTTCAACATGCAGCTTTCGGACgg                                                                                                                                                                    >  1:86329/1‑251 (MQ=255)
                                                                                              ggggCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTa                                                                                                                                                          >  1:346956/1‑250 (MQ=255)
                                                                                                         ggACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTg                                                                                                                                               <  1:184537/250‑1 (MQ=255)
                                                                                                               aTGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACt                                                                                                                                       <  1:273504/251‑1 (MQ=255)
                                                                                                                           gACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑ATgg                                                                                                                          >  1:87114/1‑251 (MQ=255)
                                                                                                                             aaaCTGACGTGCTTCAAATCACTGGAAACCGGCAACTCACGCCCGGGAGGCGAAACCGACAGCTAAAAAGCCTTCGGCTGGCTTCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGTCTAATTTACA‑CTGGAt                                                                                                                        <  2:183751/250‑1 (MQ=255)
                                                                                                                                                cACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACC‑CTGGATCACCCGCCGCGCGCCGttt                                                                                                     <  2:137264/251‑1 (MQ=255)
                                                                                                                                                 ccTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTTGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGATAACATGGTGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGGAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGACGTTTg                                                                                                    <  1:352778/250‑1 (MQ=255)
                                                                                                                                                      aaaCCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGc                                                                                                                                                                                                         >  1:4286/1‑147 (MQ=255)
                                                                                                                                                      aaaCCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGc                                                                                                                                                                                                         <  2:4286/147‑1 (MQ=255)
                                                                                                                                                          cGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGACATTCGGCCGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTACTGGCGTATTGAC‑CGACTAATTTTCAACTGG‑TCACCCCCCGCCCGCCGttttggggggca                                                                                            >  2:90046/1‑241 (MQ=255)
                                                                                                                                                                    cGCCCGGTAGGCGAAACCGACAGAGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCCGAAGGGCGGTTTCAACATGCTGCTTTCGGAAGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTCCA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTggg                                                                                 >  1:266172/1‑250 (MQ=255)
                                                                                                                                                                               cGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCCGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAAAGGCGTATTGCT‑CGGATAATTTACA‑CTGGATCACCCGCCGGGCGCCGTTTTGCGTGGCAACGGTGCTGGATAAGGCTGTgg                                                                      >  1:120856/1‑251 (MQ=255)
                                                                                                                                                                                   cccGACAGCGAAAAAGCCTTCTGCTGGCACCTGCATAAATTAACGCAGCGTTACCCGCGCACCCCGGACAACATGGCGGCTGTATTTAAATAGATCGCGTCCCTGTCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTCCA‑CGGGATCACCCGCCGCGCGCCGTGTGGCGTGGCAACGTTGATGGATCAGGATGTGGAAAt                                                                  <  2:151869/250‑1 (MQ=255)
                                                                                                                                                                                        cAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGCAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGAAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAAAGTTGCTGGATCAGGATGGGGAAATCGACt                                                             >  1:339998/1‑251 (MQ=255)
                                                                                                                                                                                            gAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTc                                                           >  1:42886/1‑249 (MQ=255)
                                                                                                                                                                                            gAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAg                                                         <  1:45135/251‑1 (MQ=255)
                                                                                                                                                                                                       ctgctgGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATCTCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGATTTTGGACGGGCGCTATGTAAAGGCGGATTGCTACGA‑TAATTTACC‑CTGGGTCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTTCTGGGTCAAGATGGGGAAATCCACTTCAGCCCCCAGAccc                                              >  2:264587/1‑250 (MQ=255)
                                                                                                                                                                                                       ctgctgGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGGTGAACTGCGGCCGAAAGGGGTTTTTCACATGCTGCTTTCGGACGGGCGCTTTGTAATAGCGTATTTCT‑CGGCTAATTTTCA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGGCACGTTTCCGTATAAGGATGTGGATATCGCATTCAGccctcacaccc                                              >  2:103628/1‑241 (MQ=255)
                                                                                                                                                                                                            ggCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCacac                                           <  1:101581/249‑1 (MQ=255)
                                                                                                                                                                                                                                             gcACACCGGGCAACATGGCGGCGGTCTTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCCGGCAGGTGTTTTCCACATCCTGCTTTCGGCCGGGCGCCATGTAATGGCGTTTTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCCCGCGCCGTTTTGGGCGGCAACGTTGCCGGCTCAGGATTGGGGAATCGACTTCAGCTCGCCGACC‑CACCCGGACGATGGGGTCACGGTGATTGCAAcacaaccc       >  2:170175/1‑247 (MQ=255)
                                                                                                                                                                                                                                                  ccGGACAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACC‑GAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGAc  <  2:42886/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                         
CGATAAAATCCTGTTCGGTGGTGGCTCATATTCGCCAGGCTAATCGGGGCGAGGTGGCGCTGGAAAATACTCACCCATTTACCCGCGAGTTATGGGGGCGTAACTGGACTTATGCCCATAACGGACAACTGACGGGCTACAAATCACTGGAAACCGGCAACTTCCGCCCGGTAGGCGAAACCGACAGCGAAAAAGCCTTCTGCTGGCTCCTGCATAAATTAACGCAGCGTTACCCGCGCACACCGGGCAACATGGCGGCGGTATTTAAATATATCGCCTCACTGGCGGATGAACTGCGGCAGAAGGGCGTTTTCAACATGCTGCTTTCGGACGGGCGCTATGTAATGGCGTATTGCT‑CGACTAATTTACA‑CTGGATCACCCGCCGCGCGCCGTTTGGCGTGGCAACGTTGCTGGATCAGGATGTGGAAATCGACTTCAGCTCGCAGACCACACC‑GAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGAC  >  NC_000913/244529‑245021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑