Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	110.4 / NA	37	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/17);  total (20/17)

TCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAA  >  NC_000913/1301746‑1302237                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       acAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATaa                                                                                                                                                                                                                                                    <  2:62612/250‑1 (MQ=255)   aaCTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                   >  1:165328/1‑250 (MQ=255)         aCCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGc                                                                                                                                                                                                                                            >  1:378769/1‑251 (MQ=255)                                 gaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGa                                                                                                                                                                                                                   <  1:365591/251‑1 (MQ=255)                                   aaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGaa                                                                                                                                                                                                                  >  1:40682/1‑250 (MQ=255)                                        ggCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGaaaaa                                                                                                                                                                                                               >  1:119137/1‑248 (MQ=255)                                            aaaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                             >  1:129590/1‑246 (MQ=255)                                            aaaaaTGGACCCAACCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCTGTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GAGCGAATTGTTCCAGTAGCTGAAAAAAGAGATc                                                                                                                                                                                                        <  2:372514/251‑1 (MQ=255)                                                        aGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGtt                                                                                                                                                                                            >  1:375427/1‑251 (MQ=255)                                                               tAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTg                                                                                                                                                                                     >  1:286774/1‑251 (MQ=255)                                                                aaTATCGTCACCAACGGTTCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGGTCACGTTGa                                                                                                                                                                                    <  2:214062/251‑1 (MQ=255)                                                                        cACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAc                                                                                                                                                                             <  1:311373/250‑1 (MQ=255)                                                                         cccAACGGTGCCTATACCTTAAAAGATTGTGTCGTAAACGAACGAATCGTTCCTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAAGTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATTCCt                                                                                                                                                                           <  1:377632/250‑1 (MQ=255)                                                                          ccAACGGTACCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtg                                                                                                                                                                          <  2:210801/251‑1 (MQ=255)                                                                               ggTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                     >  1:45112/1‑251 (MQ=255)                                                                                    cTATACCTTAAAAGATTGGGGCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCCTGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGCTCCCGGACGCAGTTCACGTTGATCCATACCTGTGCACTTACt                                                                                                                                                                >  1:261869/1‑251 (MQ=255)                                                                                               aaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAtt                                                                                                                                                     >  1:79307/1‑251 (MQ=255)                                                                                                                    gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGCGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAACTTAACAACCAGAAACCGCCATTc                                                                                                                                >  1:240849/1‑251 (MQ=255)                                                                                                                    gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGAGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTAGGAAATTAACACCCAAAAACCGCCATTc                                                                                                                                >  2:7778/1‑251 (MQ=255)                                                                                                                            cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgt                                                                                                                        >  1:293621/1‑251 (MQ=255)                                                                                                                                     aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                                               >  1:46396/1‑251 (MQ=255)                                                                                                                                      gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCCACGATgtgcgtgtgcgt                                                                                                              >  2:68055/1‑251 (MQ=255)                                                                                                                                         cGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCa                                                                                                                                             <  1:165981/217‑1 (MQ=255)                                                                                                                                         cGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCa                                                                                                                                             >  2:165981/1‑217 (MQ=255)                                                                                                                                                       aacGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGACACCGCCATTCAACGATGTGCGTGTGCGAACCGCATTGAAACTAgg                                                                                             >  2:369687/1‑251 (MQ=255)                                                                                                                                                                 ccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAACCTAGGAATGGACcgc                                                                                   >  2:244858/1‑251 (MQ=255)                                                                                                                                                                  cGTTATTAACCAGTTAACTTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGGGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTCCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTGGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACcgcg                                                                                  <  2:286774/251‑1 (MQ=255)                                                                                                                                                                     tattaACCAGGTACCCTATTTGCCTATTGCTTCGGAAGTTACCGAGGTCAACCGCTACCGTAGTGGTAAAACCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTCCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGat                                                                                <  2:119137/250‑1 (MQ=255)                                                                                                                                                                      attaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCACCCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GACCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGatat                                                                              <  1:185287/251‑1 (MQ=255)                                                                                                                                                                         acccAGTTAACCTATTTGCCTTTTGCTTCTGAAGTTACCGATGTCCACCGCTACCTTTGTGGTAAATTCGACAGGACGTATAACAG‑ATGCCCGATCGAATTGTCCCAGAAGCTAAAAAAAGATACCCCGGACGAAGTTCTCGTGAATCCATCCCTGTGCACTTCCTATTCCAAAATTAACACCAAGAATCCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCAt                                                                           <  2:204132/249‑1 (MQ=255)                                                                                                                                                                                                    tCTGAAGTTACCGATGCCAACCGCTACCGTAGGGGTGAAAACTACATGACGTATAACAGCATGCC‑GACCGAATTGTACCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGTACTTACTTTTCCGAAATTAAAAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGAGATCATTGTTAATAAAGTGAAAGCGCAGGGc                                                  <  2:161829/249‑1 (MQ=255)                                                                                                                                                                                                                aattccAACCGCTACCGTAGTGGGAAAATCGAATTGACGTATAACAGCATGCC‑TATCGAATTGTACCAGAATCTGAAAAAAGAGATCCCGGAAGAAGTCCACGTTGACCAATACCTGTGCATTTACTATTACAAAATTAACAACCAGAAACCGCCATTCAACGAGGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTa                                    <  2:365411/246‑1 (MQ=255)                                                                                                                                                                                                                 aTGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTAt                                   >  1:305632/1‑251 (MQ=255)                                                                                                                                                                                                                               cGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATg                                                                                         >  2:210957/1‑183 (MQ=255)                                                                                                                                                                                                                               cGTAGTGGTGAAATCGACATGACGTATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATg                                                                                         <  1:210957/183‑1 (MQ=255)                                                                                                                                                                                                                                  ggggggaaaatcaaaaTGACGTAAAACAGCAGGCC‑GATCGATTTGTTCAAAAAGCTAAAAAAAGAGACCCCGGACAAAGTTCCCGTGGACCAATACCTGTCAATTTACTATACCGAAATAAACAACAAGAAACCGCCATTCAACGATGTGCGTGTGCGTCCCGCACTGAAACTAGGTATGAACCGCGATATCATTGTTAATAAAGGGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGta                  <  1:7778/236‑1 (MQ=255)                                                                                                                                                                                                                                                  ggACGTATAACAGCATGCG‑GACCGAATTGTTCAAAAAGCTAAAAAAAGAGATCCCGGACAAAGTTCCCGTTGACCCATCCCGGTGCACTTACTATTACGAAATTAACAACCAAAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCaaaa  <  1:74214/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCC‑GATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAA  >  NC_000913/1301746‑1302237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑