Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	91.7%	27.2 / NA	12	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (2/9);  minor base A (1/0);  total (3/9)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGC  >  NC_000913/1195980‑1196376                                                                                                                                                                                                                                                 |                                                                                                                                                             gCAACAGATCCTGCTGCGTCCGGCTGAATATGATGGTATCGCCTGTATGAACCTGAACGGTGACTACATTGCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCTCTGGTGCAAACATCGGTGACGAATGCGCTCTGTTTGAAGCCACCCACGGTACTGTGCCGAAATATGCCGGTCAGGACAAAGTAAATCCGGGCTCTATTATTCTATCCGCTGAGATGATGCTGCGCCATATGGGTTgg                                                                                                                                                     <  1:91384/250‑1 (MQ=255)      cgaacatgcttcGTCCGGCTGAATATGAAGGTATCGTCTGGATGAATCTGAACGGTGCCTACATTTCCGACGCCCGGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACTGTACTGCGCCGACATATGCCGGTCAGGACAAAGTAAATCCTTGCTCTATTAATCTCTCCGCTGTGATGATGCTGCGCCATATGGGTTGGAcggac                                                                                                                                               <  2:97059/245‑5 (MQ=255)            tgctgcGTCCGGCTGAATATGATGTTATCGCCTGTATGACCCTGAACGGTGCCTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGCAGCCAACCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATACTGGCACTATTATTCTCTCCGCTGCGACGGTGCTGCGCCAATTGGGTTGGACTGAAGCGGcc                                                                                                                                         >  2:147355/1‑250 (MQ=255)            ggatgggttcGGCTGTATATGATGTTATCGCTTGTATGAACCGGGACGGGGACGACAGTTCTGACGCCATGGCAGGGCAGGTGGGCGGTATCGGTAACGCCCCTGGTGCAAACATCGGTGACGAATGCGCTCGGTTTGAAGCAACCCACGGTAATGCGACGAAATATGCGGGTCAGGGCAAAGTAAATACTGGCTCGATTATTCTCTCTGCTGAGATGATGCTGCGCCATATGGGGTGGACTGAAGCGGcc                                                                                                                                         <  2:44012/242‑2 (MQ=255)                          gAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGtt                                                                                                                             <  2:103614/249‑1 (MQ=255)                                           cTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCa                                                                                                           >  1:36842/1‑250 (MQ=255)                                                       gAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgccaa                                                                                                <  2:141189/249‑7 (MQ=255)                                                                            cGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac                                                                         <  1:102603/251‑3 (MQ=37)                                                                                              tGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGTAGCCACCCACGGTACTGCGCCGAAATATGACGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGAAATCAATGCCAAGACCGTAACTTATGGCTTCGAACGTCTGATGGAAGGCGCt                                                        >  2:165429/1‑250 (MQ=21)                                                                                                      ccggtatcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACCGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGctgctg                                               <  2:205286/250‑1 (MQ=21)                                                                                                                                   gACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGatca                   <  1:18914/250‑1 (MQ=17)                                                                                                                                                    tGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACATGTAAtct  <  1:249802/250‑3 (MQ=14)                                                                                                                                                                                                                                                 |                                                                                                                                                             GCAACAGATCCTGCTGCGTCCGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGC  >  NC_000913/1195980‑1196376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑