Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 100,946 T→C M61T (ATG→ACG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913100,9460TC98.1% 171.1 / NA 52M61T (ATG→ACG) murCUDP‑N‑acetylmuramate‑‑L‑alanine ligase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (23/28);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTAC  >  NC_000913/100711‑101187
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 
attggaactattccTTTTGCAGCGGATGCATTAAAGAAGTTAATGGCTTAAAGAATGAATACCCAACAATTGGCATAACAGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATCTTGCCGGCATTGGGGGTACCGGTATGGGCTGTAATGCCGAAGTGCGGGCCAATGAAGGTGATCAGATAAGTGGTTCCGATCTAGTGCCAAATCCGTCCACGCAGTAGGGAACGAATCTGGGTGcgac                                                                                                                                                                                                                                    <  2:159439/239‑1 (MQ=255)
  tGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGcgacgg                                                                                                                                                                                                                                  >  1:26129/1‑250 (MQ=255)
  gttAGCGATGCCTTTTGTATCGGATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAACTGGCAAAACTGCGGTCCATCGTGCCCGAAATGCGACGCGTTCGGCACAGACATTTTGTCGGCATTGGGGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGCTTATCAGATCAGTGGTGCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGcgacga                                                                                                                                                                                                                                  <  2:46480/249‑1 (MQ=255)
   gTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCCCATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCACTGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAACAGTTAACGCATCTggtggcgaccat                                                                                                                                                                                                                                 >  2:109700/1‑241 (MQ=255)
        gATGCCTTTTGCATCGTATGAATTTAAGCAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTATGGCCAATGATGGTTATCCGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGCTAACGAAGCAGGGAGCGACGatttatt                                                                                                                                                                                                                            >  2:256223/1‑251 (MQ=255)
              ttttGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATTAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTg                                                                                                                                                                                                                                        <  1:119275/233‑1 (MQ=255)
              ttttGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTgagatcggagagcgtcgtg                                                                                                                                                                                                                      >  2:119275/1‑233 (MQ=255)
                  gCATCGTATGAATTTAAGAAGTTAAGCGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATc                                                                                                                                                                                                                   <  2:254910/250‑1 (MQ=255)
                      cGTATGAATTTAAGAAGTTAAAGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTGCGGCACAGACAGTGTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGTTTCCGATGTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAAGCTGGGTGCGACGATTTATTTTATCCATCGCCCg                                                                                                                                                                                                              <  2:170670/251‑1 (MQ=255)
                          tGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGaa                                                                                                                                                                                                           >  1:48761/1‑250 (MQ=255)
                                               gTAAAGAATGAATACACAACAATTGGCAAAACTTCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCGTTGGTGGTGCCGGAATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCCCGCCTCAGTTAACGAGTCTGGGTGCGCCGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTgg                                                                                                                                                                                     >  2:287928/1‑251 (MQ=255)
                                                    gaatgaatACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCgtt                                                                                                                                                                                >  2:59464/1‑251 (MQ=255)
                                                                       ggCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCt                                                                                                                                                              <  2:207004/250‑1 (MQ=255)
                                                                           aaaCTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTAACGCAGCAGTTAACGAATCTGGGTGCGACGATTTCTTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGCTTTCTGCCg                                                                                                                                                          >  2:292747/1‑250 (MQ=255)
                                                                             aCTGCGTTCCATCGTGCCCGAAATGCGTGGCGTTCGGAACATACATTTTGTCGGCATTGGCGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCGAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGCTAACGAATCTGGGGGCGACGACTTATTTCAACCATCCCCCTGAAAACGTACGTGATGCCAGCGTGGGCGGTGTTTCCAGCGCGATTTCTGCCgtt                                                                                                                                                        >  2:359609/1‑248 (MQ=255)
                                                                               tGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAAc                                                                                                                                                     >  1:135289/1‑251 (MQ=255)
                                                                                 cGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGCTTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTCTTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAAccc                                                                                                                                                   >  2:379457/1‑251 (MQ=255)
                                                                                 cGTTCCATCGGTCCCGAAAGGCGTCGCGTTCGGCACATTCATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAAccc                                                                                                                                                   <  2:281770/251‑1 (MQ=255)
                                                                                         cGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAAtt                                                                                                                                            <  1:67443/250‑1 (MQ=255)
                                                                                                   aTGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACACGGAAATTGTCGCCGCTcc                                                                                                                                 >  2:3306/1‑250 (MQ=255)
                                                                                                       gtcgggggcGGCACATCCAGTTTGTCGGCAGTGGTGGTGCTGGTATGGGCGGTATTGCCGAAGTTCCGGCCAATGAAGGTTATCAGATCAGGGGTTCCGATTTAGCGTCAACTCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCATCCATCGCCCGTAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGACTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGaa                                                                                                                             <  2:82880/243‑1 (MQ=255)
                                                                                                       gTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGCTTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGa                                                                                                                              >  2:7494/1‑250 (MQ=255)
                                                                                                       gTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGaa                                                                                                                             <  1:199721/251‑1 (MQ=255)
                                                                                                              tCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGCAATTGACGCCGCTCCTGAAGCGCGt                                                                                                                       >  1:4099/1‑250 (MQ=255)
                                                                                                                 gcccattcatTTGGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTGAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTTGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCTTATTc                                                                                                                   <  2:160470/244‑1 (MQ=255)
                                                                                                                           tttGTCGGCATGGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGACAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGTTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTCCGTGATGCCATCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAAGTGTCGCCGCTCATGAAGTGCGTATTCCGGTGATcc                                                                                                          <  1:289593/250‑1 (MQ=255)
                                                                                                                              gTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtcg                                                                                                      <  1:345664/251‑1 (MQ=255)
                                                                                                                              gTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGAACAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCcgtc                                                                                                       <  1:30516/250‑1 (MQ=255)
                                                                                                                                    aTTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCg                                                                                                 >  1:346652/1‑250 (MQ=255)
                                                                                                                                        gtggtgCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATg                                                                                            >  2:359046/1‑251 (MQ=255)
                                                                                                                                          ggtgCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgc                                                                                           <  1:196074/250‑1 (MQ=255)
                                                                                                                                              ccGGTATGGGAGTGATGGCCGAAGTTCTGGCCAATGAATGTTAGCAGATCAGTGGTTCCGAGTTAGCGCCAAATCCGGGCACGCAGCAGTTAATGAAGCTGGGGGCGAAGATTTATTTCAACCCTCGCCCGGAAAACGTACGTGATGCCAGGGTGTGCGTTGTTTCCAGCGCTATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGTTGATCCGTCGTGCCGAAATgctggct                                                                                      <  2:13043/251‑1 (MQ=255)
                                                                                                                                                   aTGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATTAAGCGCGTATTCCGGTGATGCGTCGTGCCGAAATGCTGGCTGAGtt                                                                                 <  1:337349/251‑1 (MQ=255)
                                                                                                                                                       gCGGTATTGCCGAAGTTCTGGCCAATGCAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATg                                                                             <  2:256857/251‑1 (MQ=255)
                                                                                                                                                          gTATTTCCGAAGTTCTGGCCAATGATGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCTGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGt                                                                          <  1:66145/251‑1 (MQ=255)
                                                                                                                                                           tATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggctg                                                                                     >  1:15577/1‑239 (MQ=255)
                                                                                                                                                           tATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATgctggctg                                                                                     <  2:15577/239‑1 (MQ=255)
                                                                                                                                                                          ggcaaaTGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCTCGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCTGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATc                                                           <  2:274791/246‑1 (MQ=255)
                                                                                                                                                                            ccAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGcc                                                        >  1:20847/1‑251 (MQ=255)
                                                                                                                                                                               aTGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCGTCGCCAtt                                                     <  1:229240/251‑1 (MQ=255)
                                                                                                                                                                                   aGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCg                                                 <  1:109700/251‑1 (MQ=255)
                                                                                                                                                                                           agatcagTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCAc                                         <  1:59464/251‑1 (MQ=255)
                                                                                                                                                                                                        cGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGAGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGACGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACa                             >  2:56795/1‑250 (MQ=255)
                                                                                                                                                                                                         gATTTAGCGCTAAAGCCGGGCACTTAGCAGTTAACTAAGCGGGGTGCGACGATATATTTCAACCAGCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGGTTCCAGCGCTATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCAGGAAGCGCGTATTCCGGTGAGCCTTCGTGCCGAAATGCTGGCTGAGTTAAGGCGTTTTCGTCATTGCATCGCCATTGCCGGAACGCACTGCAAAACGACAac                           <  2:242936/251‑1 (MQ=255)
                                                                                                                                                                                                         gATTTAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTAGCCGCTCATGAAGCGCGTATTCCGGTGCTCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTAGTCCTGGCCTCGCCATTGCCGGAACGCACGGCAAAACGACaa                            >  2:198283/1‑250 (MQ=255)
                                                                                                                                                                                                            ttAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACTATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacca                         <  2:48761/250‑1 (MQ=255)
                                                                                                                                                                                                            ttAGCGCCAAATCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGAGTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGGCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAacca                         <  2:264301/250‑1 (MQ=255)
                                                                                                                                                                                                                    aaaTCCGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATgg                <  2:26129/251‑1 (MQ=255)
                                                                                                                                                                                                                        ccGGTCACGCAGCAGTTAACGAATCGGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTc            <  2:267603/251‑1 (MQ=255)
                                                                                                                                                                                                                         cGGTCACGCAGCAGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTcc           >  1:49943/1‑251 (MQ=255)
                                                                                                                                                                                                                                gcagcaGTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCt    >  1:282760/1‑251 (MQ=255)
                                                                                                                                                                                                                                  agcagTTAACGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCtcc  >  1:84794/1‑249 (MQ=255)
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ATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATCGTGCCCGAAATGCGTCGCGTTCGGCACATACATTTTGTCGGCATTGGTGGTGCCGGTATGGGCGGTATTGCCGAAGTTCTGGCCAATGAAGGTTATCAGATCAGTGGTTCCGATTTAGCGCCAAATCCGGTCACGCAGCAGTTAATGAATCTGGGTGCGACGATTTATTTCAACCATCGCCCGGAAAACGTACGTGATGCCAGCGTGGTCGTTGTTTCCAGCGCGATTTCTGCCGATAACCCGGAAATTGTCGCCGCTCATGAAGCGCGTATTCCGGTGATCCGTCGTGCCGAAATGCTGGCTGAGTTAATGCGTTTTCGTCATGGCATCGCCATTGCCGGAACGCACGGCAAAACGACAACCACCGCGATGGTTTCCAGCATCTAC  >  NC_000913/100711‑101187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑