Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,069,743 C→T A33T (GCA→ACA)  rutG ← pyrimidine:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,069,7430CT95.5% 110.8 / ‑6.3 44A33T (GCA→ACA) rutGpyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (29/13);  total (29/15)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

GCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACG  >  NC_000913/1069510‑1069977
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
gCTGTTATCCGGTTTAATGCCCTGACCGTAAAACCCCGCCGCGGCGATCCCCACCCCGACAAAGCCCGCGGTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGc                                                                                                                                                                                                                             <  1:144544/250‑1 (MQ=255)
 cTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACACAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTTTCGTCTGGGCAAACGGCa                                                                                                                                                                                                                            >  2:245117/1‑250 (MQ=255)
                           gTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCGTTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCAcgccgc                                                                                                                                                                                                >  1:274053/1‑251 (MQ=255)
                              aaaGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCCTTACTGTCGTCTGAGCAAACGGCACTCATTCA‑TCCGGCGCGGCCCcgccgccct                                                                                                                                                                                             >  2:126666/1‑248 (MQ=255)
                                                        cGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAACATGGAAAGATTGGGATCCAGCCCCATCAGAATAGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAACAGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAAGTAGATTTTAACTGcc                                                                                                                                                                     >  2:111121/1‑249 (MQ=255)
                                                               gccgcggtgggaccgaaaacagggggggAGGCCCCCGCCGGTAATAAAGAAAAACATTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGTCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACGGTCGTCTGAGAAAACGGCAATCTTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGaa                                                                                                                                                            <  1:261802/227‑1 (MQ=255)
                                                                              ataaacacgggggCCGCCCCCGCCGGTAATAAAGAAAACCAGTACCGTGCCGACCCCCGACATAAAAATGGAAAGATTGGGATCCAGCCCCATCAGAACCGCCATTAACCCCTCCGCGCCAAACATCGCC‑ACCGCGTTTTGAACTCCAATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑CCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCa                                                                                                                                             <  1:344547/242‑1 (MQ=255)
                                                                               agaTAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCAt                                                                                                                                            >  1:132289/1‑251 (MQ=255)
                                                                                              cgcccgccTGTGAAAAAGAAAAACAGTACCGTGCCGATCCCCGACATTACAACGGAAAGATTGGAATCCAGCCACATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAGCGCCCATTACTGTCGTCTGAGCACACGGCAATCGTTCA‑TCGGACGCGACCCCGCCTCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGACAAACGAACATTACCTTCAGCtctttcattat                                                                                                                             >  2:360952/1‑241 (MQ=255)
                                                                                               gcccgccGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTa                                                                                                                              >  2:308282/1‑249 (MQ=255)
                                                                                                 ccgccgGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGAATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGCGGTCGCTTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGGCTCCTTAaggg                                                                                                                          >  2:298796/1‑250 (MQ=255)
                                                                                                        aaaaaaaGAAAAACAGTAACGGCCCGACCCCCGACATAAAAAGGGAAAGATTGGGATCCAGCCCCACAAGAAGCGGAATTAACACCGTCGCGCAAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAACCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAAc                                                                                                                   <  1:126666/247‑1 (MQ=255)
                                                                                                         aataaAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTACCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAAc                                                                                                                   >  1:224760/1‑250 (MQ=255)
                                                                                                          atgaaGAAAAACAGTAACGTGCCGCTAACCGACATTAAAATGGAAAGATTGGGATACCGCCCCATCAGAATCGGCATTAACACCGTCGCGCAAAACATCGCC‑ACCGCGTGTTGAACGCCCATTCCTGTCGTCAGAGCAAACGGACATCGTTCA‑TAAGGCGCGCCCACGCCGCTCTCTGTAGAGGTAGCTTTTAACTACCAGTGAGGAAAACCGAACATTGCCTTCAGCTGACTCCATGAGGAGGTTacaa                                                                                                                  >  2:240669/4‑247 (MQ=255)
                                                                                                               gAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAAAAAGAAgg                                                                                                            >  2:379595/1‑251 (MQ=255)
                                                                                                                                 gATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGAt                                                                                           >  1:174043/1‑250 (MQ=255)
                                                                                                                                 gATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGAt                                                                                           >  1:69164/1‑250 (MQ=255)
                                                                                                                                 gATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGGGAt                                                                                           >  2:283820/1‑250 (MQ=255)
                                                                                                                                 accccccGACATTAAAATGAAAAGACTGGGATCCAGCCCCGTCAGAACTGGCATTCACACCTCCGCGCCACACACCGCC‑CCGGCTTGTTGAACGCCCATTACTGTCGTCTGAGCAATCGGCAATCGTTCC‑CCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATa                                                                                          <  2:25353/248‑1 (MQ=255)
                                                                                                                                  cccccggACATAAAAATGGAAATATTGGAATCCAGCCCCAACAGATCCGTCCTAAACACCGGCGCGCCAACCATCTCC‑CCCGCGTGTGTATCGCCCATTACTTCCTTGTTAGCACACGGCAATCGTTCA‑TCCGGCGCGCCCACGCCGCTCTCTGAAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGAt                                                                                           <  2:212747/243‑1 (MQ=255)
                                                                                                                                    ccccGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACt                                                                                       >  1:202923/1‑251 (MQ=255)
                                                                                                                                        gACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCg                                                                                    >  1:60718/1‑250 (MQ=255)
                                                                                                                                               aaaTGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACAGCGCC‑ACCGCGTGTTAAACGCCAATTACTGCCTCCTGATAAAACGGCAATCGTTCA‑TCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAAcc                                                                            <  1:148107/251‑1 (MQ=255)
                                                                                                                                                aaTGGAAAGATTAGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTACCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGGGATTACTGCCATCGAAccc                                                                           >  1:80404/1‑250 (MQ=255)
                                                                                                                                                              gATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAgg                                                             >  2:34919/1‑251 (MQ=255)
                                                                                                                                                                tCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGACCATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCCTCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAgggg                                                           >  2:91295/1‑250 (MQ=255)
                                                                                                                                                                tCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTa                                                                                                                              >  1:104187/1‑184 (MQ=255)
                                                                                                                                                                 ccAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGggtg                                                          >  1:346155/1‑251 (MQ=255)
                                                                                                                                                                  cAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGggtgt                                                         >  1:251347/1‑251 (MQ=255)
                                                                                                                                                                  cAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACACCGCC‑ACCGCGTGTTGACCGCCCTTTACTGTCGTCTGAGCAACCGGCAATCTTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGggtgt                                                         <  1:316794/251‑1 (MQ=255)
                                                                                                                                                                            aGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCAGCCCAGCCCGTAGGGTGTGGTGTGACg                                                >  2:252307/1‑250 (MQ=255)
                                                                                                                                                                             gAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGAt                                              >  1:8220/1‑251 (MQ=255)
                                                                                                                                                                              aaTCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGGGGGGTGTGACGATg                                             >  2:284477/1‑251 (MQ=255)
                                                                                                                                                                                  ggtaTTAACCCCGTCGCGC‑AAACCTCCCCCACCGCGTGTTGAACCCCCATTCCTGTCGTCTGAGCAAACGGCAATC‑TTCAACCCGGCGCAACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATc                                         <  2:342530/248‑1 (MQ=255)
                                                                                                                                                                                        aaCACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGGGTGGTGTGACGATGAATCGCTTCg                                   >  2:317966/1‑251 (MQ=255)
                                                                                                                                                                                          ccccGTCGCGCCAAACACCGCC‑CCCGCGTGTTGACCGCCCATTACTGTCGTCTGAGCAAACGGCAACCGTTCA‑CCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGAt                                 <  1:58747/249‑1 (MQ=255)
                                                                                                                                                                                           acCGGCGCGCAAAACATCGCC‑ACCGCGTTGTGAACGCCCATTCCTGTCGTCTGAGCAACCGGCAATCGTTCA‑TCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTCAGGAGGTTAACAAGCCGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTCGGTTGTGGTGTGACGATGAATCTCTTCgttg                                >  2:42492/1‑248 (MQ=255)
                                                                                                                                                                                              gTCGCGCCAAACATCGCC‑ACCGCGTGTTGACCGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGgcg                             <  1:284477/251‑1 (MQ=255)
                                                                                                                                                                                                cgcgCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCTTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCa                           <  1:91295/251‑1 (MQ=255)
                                                                                                                                                                                                   cccAAACACCGCC‑ACCGCGTGTTGACCGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑CCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCaaaa                        <  1:34919/250‑1 (MQ=255)
                                                                                                                                                                                                    ccAAACACCCCC‑CCCGCGTTGTGAACGCCCATCACTGTCGTCGGGGATAACGGCAATGGTTCA‑TCGGGGGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCGCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAAc                       <  2:274053/251‑1 (MQ=255)
                                                                                                                                                                                                                          tGAACGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACg  >  1:5404/1‑251 (MQ=255)
                                                                                                                                                                                                                              cGCCCTTTCCTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGCCCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtgg                                                       <  1:142277/194‑1 (MQ=255)
                                                                                                                                                                                                                              cGCCCATTACTGTCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGggtgtgg                                                       >  2:142277/1‑194 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
GCTGATATTCGGGTTAATGCCCTGACCGTTAAAGCCCGTCGCGGCGATCACCACGCCGACAAAAGCCGCGCTGGAGCCGAGATAACTGGGGACGCGCCCGCCGGTAATAAAGAAAAACAGTAACGTGCCGATCCCCGACATTAAAATGGAAAGATTGGGATCCAGCCCCATCAGAATCGGCATTAACACCGTCGCGCCAAACATCGCC‑ACCGCGTGTTGAACGCCCATTACTGCCGTCTGAGCAAACGGCAATCGTTCA‑TCCGGCGCGACCACGCCGCTCTCTGTAGAGGTCGATTTTAACTGCCAGTGAGGAAAACCGAACATTGCCATCAGCTGTCTCCTTAAGGAGGTTAACAAGCAGGGCGCATCAGCGCGTGATAACTGCGATCGAACCACACCAGCCCGTAGGGTGTGGTGTGACGATGAATCGCTTCGATGGCGCAAAACAGAATGTCGTGGGTGCCGACG  >  NC_000913/1069510‑1069977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑