Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,061,935	G→A	S46N (AGC→AAC)	torD →	trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,061,935	0	G	A	91.7%	91.9 / ‑3.0	38	S46N (AGC→AAC)	torD	trimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (19/14);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

GTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAC‑AAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATC  >  NC_000913/1061692‑1062171                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               gtggAAATTGAGAAGTATAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGAGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTAAGCCTGGCTAGCGCAGTTGTTCTCCCGTGCGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGAAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAGCGAAccg                                                                                                                                                                                                                                         >  2:158835/1‑251 (MQ=255)                   aaCGGAACAGGGGAGCGGGGGACGGCGTTTAACGGCCCCGTGGAGATGGTGGGGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGCCCAC‑GCTGACAGCACAACAGATGGCCTGT‑TTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGAAAA‑TCGCCAGGGCGCAGAGGGCTGAATGTTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTg                                                                                                                                                                                                                       <  1:226901/251‑1 (MQ=255)                      ggAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGaa                                                                                                                                                                                                                     >  1:376867/1‑250 (MQ=255)                         aCAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTG‑CGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACg                                                                                                                                                                                                                   >  2:80009/1‑249 (MQ=255)                               gagcagacgACGGCGTTTAACGGACCCGTGGAGGTGGTGGCGCCGCGAGCATATGTTACCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCATGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAA‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGCATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGGACGAGATggg                                                                                                                                                                                                            >  2:162084/9‑249 (MQ=255)                                   gggTGACGGCGTTAACCGGCCCCGTGGAGAGGGTGGCGCAGGGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGCCCAC‑GCGGACAGCACAACAGATTGCCTGT‑GTTTACGCCGGGCTAGCGCAGTTGTTCTCCCGGGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGTTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAAcc                                                                                                                                                                                                       <  1:15497/250‑1 (MQ=255)                                       gACGGCGTTTACGGGCCCCGTGGGGATGGTGGCGCAGTGCGATTTGGTCCCCGGGCCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGC‑TGTTGTTTCCGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGAGGAT‑AAACAACTGACGAAAT‑TCGCCTGTCCGAAGAGGGCTGATTGGTTTT‑CTTTGCTTAAAACCAGAGCGACGCTCACTGCTGCGGTGAACGAGCTGGAAAACCGTAt                                                                                                                                                                                                   <  1:305387/251‑1 (MQ=255)                                        gcGGCGTTTACCGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTCCCCGCGTCGCAGGTAAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCCCCCGTGAGCTGGACGAT‑GAACAACTGACGAAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTAtt                                                                                                                                                                                                  <  1:306771/250‑1 (MQ=255)                                        gaggggttgacgggccggggggAGATGGTGGCGCAGGGGGAATATGTGCCCGCGTCGCAGGGAAAA‑TCAGGACCCC‑GCGGACAGAAACATGGATGGCGTGG‑GGTTACCCCTGGCGAGCGCTGTTGTGCTCCGGGGGTGGGGACGAT‑GAACAACTGACGAAAA‑TCGCCAGGGCGAAGATGGGTGATGGGTTTT‑CGTGGCAAAAAAACGATCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTAtt                                                                                                                                                                                                  <  2:226825/231‑1 (MQ=255)                                            cGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGGAAACCGTaattcc                                                                                                                                                                                               >  1:94899/1‑245 (MQ=255)                                                   cGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGGGGACGAGCTGGAAAACCGTATTTCCCCGCTggc                                                                                                                                                                                       >  2:81461/1‑249 (MQ=255)                                                         gggggggagtgtggCGCAGTGCAAATATGTTCCGGTGGCGCAGG‑GAAACGCGGGCCCGC‑GCTGAGAACACAACAGATGGCCGGT‑GTTTACGCCGGGTGAGCGCAGTTGTTCCCGCGTGAGCGGGACGAT‑GAACAACGGACGAAAA‑TCGCCGGGGCGCAGAGGGCTGAAGGGTTTT‑CGTTGCAAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTa                                                                                                                                                                                   <  2:181359/239‑1 (MQ=255)                                                                aTGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCCGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAATACGAACCGCCGCTCACTGCGGCGGTGCACGAGCTGGGAAACCGTATTTCCACCCTGACAGACCGTGACGAt                                                                                                                                                                          >  1:122965/1‑251 (MQ=255)                                                                 gggtgccgcgtggCGAATAGGTTCCCGCTTCGCGGGTGAAA‑TCATGCCCAC‑GCTGACACCACAACAGATGGCCTGT‑GTTGCCGCCTGGCTAGCGAAGTTTTCCCCCCGGGAGTTGGACGAT‑GAAAAACGGACGCAAA‑TCGCCGGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTAAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATg                                                                                                                                                                         <  1:77324/239‑1 (MQ=255)                                                                     ggCGCAGTGCGAGTATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGGT‑GAACAACTGGCGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGATCACGGCGGCGGTGGAAGAGCTGGAAAACCGTATTGCACCGCTGACAgatctgggagattgccg                                                                                                                                                                     >  2:72555/1‑235 (MQ=255)                                                                           gTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTggg                                                                                                                                                               >  1:173445/1‑250 (MQ=255)                                                                               gAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGaaatg                                                                                                                                                           >  1:203174/1‑248 (MQ=255)                                                                                        cccGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGAc                                                                                                                                                  >  1:289990/1‑251 (MQ=255)                                                                                          cgcgTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACtt                                                                                                                                                >  1:16828/1‑251 (MQ=255)                                                                                           cgggcGCAGGTAAAA‑GCATGCCCCC‑GCTGAAAGACCAACAGATTGCCTGT‑GTTGCCGCCGGGCGAGCGCAGTTGTTCCCCGGGGAGC‑GGGCGGTAGAACAACGGGGGCAAA‑TCGCCAGTGCGCAGATGGCGGAATGTTTTG‑CGTTGCGGAAAACCGACCCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACttt                                                                                                                                               <  2:170120/247‑1 (MQ=255)                                                                                                    gtgaaaaccattccctc‑GCTGACAGCACAACAGATTGCGTGT‑GTTGACGCTTGCCTAGCGCAGTTGTTCTCCCGTGAGTGGGACGAT‑AAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGATTGTTTTT‑CGTTGCTAAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCt                                                                                                                                       <  1:212356/235‑1 (MQ=255)                                                                                                    gTGAAT‑TCATG‑CCACCGCTGACAGCACACCAGTTTGCTTGG‑GTTTCCGCTTGGCTAGCGCAGTTTTTCTCCGGTGAGCGGGACAAT‑AAACAACTGACGAAAA‑TCGCAAGGGCGCAGATGGCTGAATGTTTTT‑CGTTGCTAAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTg                                                                                                                                      <  1:209840/251‑1 (MQ=255)                                                                                                    gTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGGACGAGCTGGAAAACCGTATTGCCACGCTGACATTAAGGGACGAGGCCCGTCTGGAACTAGCCGCGGACTTTTGCCGCCTg                                                                                                                                      >  2:180412/1‑251 (MQ=255)                                                                                                                             aaaaaacaGATGGCGTGT‑GTTTACGCCTGGCAAGCGAAGTGTTTCTCCCGAGAGCAGGACGAA‑AAAAAAAGGACAAAAA‑TCGCCAGGGCGAAGAGGGCTGAATGGTTTT‑CGTGGCAAAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAgc                                                                                                              <  2:91077/247‑1 (MQ=255)                                                                                                                              cacaACAGATTGCCTGT‑GTTTACGCCTGGCTGGCGCAGTTTTTCCCCCGTGAGCTGGACGAG‑GAACAACTGACGAAAA‑TCGCCGGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAgcg                                                                                                             <  1:81461/251‑1 (MQ=255)                                                                                                                                   caGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGCACCGCCGCTCACTGCGGCGGTGCACGAGCTGGGAAACCGAATTGCCACGCTGACCGAACGGGACGATGCCCGGCTGGGACCCGCCGCGGGCCTTTGCGGCCTGGTTCTGAT‑GACCGCCAAACAAgcggccat                                                                                                        >  2:18032/1‑248 (MQ=255)                                                                                                                                   caGATTGCCGGT‑GTTTCCGCCGGGCTAGCGTGGTTGTTCTCCCGGGAGCGGGACGAT‑GAACAACGGACGCAAA‑TCGCCAGTGCGCAGATGGATGAATGGTTTT‑CGTGGCTGAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCt                                                                                                        <  1:60106/251‑1 (MQ=255)                                                                                                                                        tGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAAATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGGGCTGGAAAACCGAATTTCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCCACAAACAAGCGGCGccgccgg                                                                                                   >  2:246863/1‑250 (MQ=255)                                                                                                                                                gtTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGAAAGTACGGTCCGGTGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCCGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGGATGcctc                                                                                            >  2:173879/1‑248 (MQ=255)                                                                                                                                                   tACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGGGACGATGCCCGTCTGGAACTGGCCGCGGGCTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGc                                                                                         >  1:280454/1‑251 (MQ=255)                                                                                                                                                                cgcggttgttCCCCCGTGAGGTGGACGAT‑GAACAACTGCCGAAAC‑GCGCCCGGGCGCAGATGGCGAAATGGTTTG‑CGTTGCGGAAAAACAACCCGCCGCGCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GCCCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAC‑AAACAGGAc                                                                           <  2:42552/247‑1 (MQ=255)                                                                                                                                                                          cccccGGGAGTTGGAGGAT‑AAACAACTGAC‑CAAACTCGCCGGTGCGCAGATGGTTGA‑TGGTTTTGCGTTGCTAAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAC‑AAACAGGACGAGCAagag                                                                  <  2:120520/248‑1 (MQ=255)                                                                                                                                                                                                                 gCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGACCGTCTGGAACTGGCCGCGGACTTTTGCCGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTTCCGTATGCATCGGCCTAC‑AAACAGGACGAGCAAGAGATTAAAAGCTAGTTAGTTGAGGCAGGGGTGGTAAcc                              >  1:33015/1‑249 (MQ=255)                                                                                                                                                                                                                              tGGCTGCATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACACTATTGCCACGCTGACCGTACGTGACGATTGACGTCTCGACATAGCCGCCGACTTTTCCGGCCCGGTTCTGATATACGGA‑AAACACACGGGCCATCCGTATGCATCGGGCTACCAAAC‑GGACCAGCACGAGGTTAAACGATTGGTCGTTGAGGCAAGGaggggaaacacggggaatttctcc               >  2:365555/1‑228 (MQ=255)                                                                                                                                                                                                                                  tGAATGGTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGTAAACCGTATTGCCCCGCTGACAGTACGTGACGATGCCCGTCTGGAACTTGCCGCGGACTTTTTCGGCCTGTTTCTGGT‑TACCCGCCAACAAGCGGCGCTGCCGGATGCCTCCGCCTAC‑AAACAGGACGAGCCAATGGTTAAACGCTTGTTAGTTGTGGTCGGGATGGAAACCAGCGGCACTTTCAAcagac           >  2:90568/1‑247 (MQ=255)                                                                                                                                                                                                                                        gTTTT‑CGTTGCTGAAAAACGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGTACTGGCCGCGGGCTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTTC‑ACACAAGGCGCGCCAGAGATTAAAAGCTTGCTAGTTGAGGGAGGGAGGGAAACACGAGGCAATTTCaaactacctgacg     >  2:3293/1‑240 (MQ=255)                                                                                                                                                                                                                                         tttt‑TGTTGCGGAAAAGTGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGGCTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAC‑AAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAg     <  1:80009/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccGTTGCTAAAAAACAAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAC‑AAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGatc  <  1:95553/249‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               GTGGAAATTGAGAAGTACAACGGAACAGTGGAGCAGGTGACGGCGTTTAACGGCCCCGTGGAGATGGTGGCGCAGTGCGAATATGTTCCCGCGTCGCAGGTGAAA‑TCATGACCAC‑GCTGACAGCACAACAGATTGCCTGT‑GTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGAT‑GAACAACTGACGCAAA‑TCGCCAGTGCGCAGATGGCTGAATGGTTTT‑CGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGCCCGTCTGGAACTGGCCGCGGACTTTTGCGGCCTGTTTCTGAT‑GACCGACAAACAAGCGGCGCTGCCGTATGCATCGGCCTAC‑AAACAGGACGAGCAAGAGATTAAACGCTTGTTAGTTGAGGCAGGGATGGAAACCAGCGGCAATTTCAACGAACCGGCAGATC  >  NC_000913/1061692‑1062171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑