Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	70,659	G→A	W91* (TGG→TGA)	araC →	DNA‑binding transcriptional dual regulator AraC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	70,659	0	G	A	100.0%	67.7 / NA	25	W91* (TGG→TGA)	araC	DNA‑binding transcriptional dual regulator AraC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

GGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGC  >  NC_000913/70420‑70897                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                ggATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATATCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCAACAGTg                                                                                                                                                                                                                                      >  1:309027/1‑251 (MQ=255)                                            ttGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGACTGGCTTAAc                                                                                                                                                                                           >  1:375802/1‑251 (MQ=255)                                                   cAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGt                                                                                                                                                                                    >  1:211502/1‑251 (MQ=255)                                                          tATCTCGATTTTTTTATCGACCGACCGTTGGGAATGAAAGGTTATATTCTCATTCTCCCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAatat                                                                                                                                                                              <  1:227106/250‑1 (MQ=255)                                                            catcGATTTTTTTATCAACCCGCCGTGGGGAATGAAATGTTATTTACTCACCCTCACCATTCGCGGTCAGGGGGTGGGGAAAAACCGGGGACGAAAATTTGCCTGCCGACCGGTTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGGCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTg                                                                                                                                                                           <  1:14123/249‑1 (MQ=255)                                                              tcGATTTTTTTATCGACTGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATGCGCGGGCAGGGGGTGGTGAAAAATCAGGGATGAGAATTTGTCTGCCGACCGGGTGATATTTGGCTGTTCCCGCCAGGAGAGATTCATCAATACGGTCGTCATCCGGAGGCGCGCGAATGATATCACCAGTGGGTTTACTTTCGTTCGCGCGCCTGCTGGCATGAATTGATGAACTGCACGGCAATATTTGcc                                                                                                                                                                         >  2:271290/1‑251 (MQ=255)                                                                            gaccgaccGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAAGGATATCACCAGTGGGTTTACTTTCTTCCGCGCGCCTACTGGAATGAATGGCTTAACTGGCCGTCACTATTTTCCAATTCggggttttt                                                                                                                                                           >  2:209450/1‑245 (MQ=255)                                                                                                   ttATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGc                                                                                                                                    >  1:175898/1‑251 (MQ=255)                                                                                                                        tCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt                                                                                                                <  1:167888/250‑1 (MQ=255)                                                                                                                                        gtaaaaaaTCAGGTACGAGAATTTGTCTGCCGACCGGGGGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGc                                                                                              <  1:197338/248‑1 (MQ=255)                                                                                                                                                      ccaagaATTTGTCTGCCGCCGGGGAGATATTTGGTTGTCCCCGTCAGGAGAGATTCATCACTCCGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAg                                                                                <  2:211502/248‑1 (MQ=255)                                                                                                                                                                gtcggcggagggggtgtttttttGCTGTCCCCGCCAGGAGAGATTCATCCCTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTAtt                                                                       <  2:239863/231‑1 (MQ=255)                                                                                                                                                                 tCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctttc                                                                                                                                                         >  1:354286/1‑168 (MQ=255)                                                                                                                                                                 tCTGCCGACCGGGGTATATTTGGCTGTCCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctttc                                                                                                                                                         <  2:354286/168‑1 (MQ=255)                                                                                                                                                                            ggTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTCACTGGCCGTCAATATTTGCCAATAAGGGTTTCTTTCGCCCGGCTGAAGCGCACCAGCTGCATTTCAGCAACCTGTTTGGGCAAATCATTATAC‑ACGGGCAAGGGGAAGGGCGCCAGTCGGTgctgcttgc                                                          >  2:142374/1‑248 (MQ=255)                                                                                                                                                                            ggTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGc                                                          >  1:50605/1‑251 (MQ=255)                                                                                                                                                                                  atTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATaa                                                     >  1:279447/1‑250 (MQ=255)                                                                                                                                                                                    ggggtgtgtccctcaaggaggGATTCAGCACTACGGTGGTCATCCGGTGGCTGGCGAATGATATCACCAGTGGGTTTACTTTCGGCCGCGCGTCTACTGGCATGAATGGCTTAACTGGCCGTTAATATGTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAGTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGCGCTGCTGGCGATAAAtt                                                  <  2:69470/236‑2 (MQ=255)                                                                                                                                                                                     ttgttGTTCCCGCCAGGAGAGATTCATCCCTCCGGGCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGGCCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATc                                                  <  1:20007/246‑1 (MQ=255)                                                                                                                                                                                                        agaTTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCCCCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTg                              <  1:300840/251‑1 (MQ=255)                                                                                                                                                                                                           ttCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGCCCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTgcgg                           >  1:213575/1‑251 (MQ=255)                                                                                                                                                                                                                         cgtcATCCGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGAt             >  1:121249/1‑251 (MQ=255)                                                                                                                                                                                                                             acccGGGGGCTCGATAATGATATCCCCAGGGGTTTTACTTTCGGCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTGGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAGTTCAGCGACCTGTTTGGGCAAATCAGTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTa           <  2:213575/247‑1 (MQ=255)                                                                                                                                                                                                                               ccGGAGGCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCAGTCAATATTTGCCAATACGGGTTTATTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGGCCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACGGCGGCGCATGGAAGCGATTAACgg       >  2:360380/1‑250 (MQ=255)                                                                                                                                                                                                                                    ggCTCGCGAATGATATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCCCCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCCTGGAAGCGATTAACGAGTCGc  >  2:65288/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                GGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTGGGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTA‑ACGCCGGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTACTGCGGCGCATGGAAGCGATTAACGAGTCGC  >  NC_000913/70420‑70897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑