Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,429,986	IS3 (–) +5 bp :: +T	coding (34‑38/258 nt)	yrdB ←	DUF1488 domain‑containing protein YrdB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	33 (0.920) +T	33/484	0.2	91.5%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3429986 =	3 (0.080)					coding (38/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB
*	?	NC_000913	1094245 =	NA (NA)	42 (1.170)	39/484	0.1	93.2%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3429990	3 (0.090)					coding (34/258 nt)	yrdB	DUF1488 domain‑containing protein YrdB

AAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCTCAT  >  NC_000913/1094001‑1094490                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aaaGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGGGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCt                                                                                                                                                                                                                                                 >  2:281684/1‑251 (MQ=255)               aaGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAACGTGCCGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTAACCACGTAatttg                                                                                                                                                                                                                                   >  2:78633/1‑248 (MQ=255)                                                       ccTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTcc                                                                                                                                                                                           <  1:281684/251‑1 (MQ=255)                                                          gAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCg                                                                                                                                                                                          <  1:314870/249‑1 (MQ=255)                                                             tACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATATATCGACTGGCTGATAACGCTCTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTATTGTTTTCAAATTGTTCCGGCCTg                                                                                                                                                                                     >  2:67911/1‑251 (MQ=255)                                                               cGCTAGACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAg                                                                                                                                                                                   <  1:73693/251‑1 (MQ=255)                                                                               gTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGAGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTACGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCCCACCAACtt                                                                                                                                                                   >  2:299380/1‑250 (MQ=255)                                                                                     aTTAGTATTGGCGCTGTTGTGACTCCGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgccgc                                                                                                                                                             >  1:307602/1‑251 (MQ=255)                                                                                         gTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGACCTTTACGAGGCGACAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTgccgccgcc                                                                                                                                                         >  1:174263/1‑251 (MQ=255)                                                                                                       gtgACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGTTCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCCCCGATTGTAATc                                                                                                                                            >  2:281251/1‑250 (MQ=255)                                                                                                                   aaTGGGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGAAATAAGGTGGTTGGGAGGGAGGTGGTGAATTTTTGGG‑ATTCACCATAAAGCGGCATTTTGACCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATaa                                                                                                                               <  2:211992/251‑1 (MQ=25)                                                                                                                                      agaTCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc                                                                                                                >  2:79388/1‑247 (MQ=255)                                                                                                                                      agaTCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGGGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc                                                                                                                <  1:79388/247‑1 (MQ=255)                                                                                                                                               gACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGGGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTccc                                                                                                   <  1:107217/251‑1 (MQ=255)                                                                                                                                                 gtGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGGGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCgg                                                                                                 <  2:126137/250‑1 (MQ=255)                                                                                                                                                          aaCGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATaa                                                                                          >  1:71852/1‑249 (MQ=255)                                                                                                                                                                                                 gtggtggggagtgaggtggtgAATTTT‑GTGGAGTCACCATAAAGCGTAATTTTGATCCTACCCTCTTAATAGGGACACAGGCCTAAGCGAGTGTCTTTTTTACAAATTGTTCCGTACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAaag                                                 <  2:320639/236‑1 (MQ=11)                                                                                                                                                                                                      tggtaGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTCCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGc                                             <  1:279905/246‑1 (MQ=255)                                                                                                                                                                                                          ggggagggtgtgAATTTTTGTG‑AGTCCCCATAAAGCGTCATTATGACCCTACCCACGTAATATGGCCACAGGCCTAAGCGAGGTTCTTGTTTTAAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTcc                                        <  2:222642/242‑1 (MQ=11)                                                                                                                                                                                                          aGGGATTTGGTGAATTTTTGTG‑AGTCACCATAAAGAGGCATTTTGATCCTACCCACGTAATATGGTAACAGGCCTAAGCGAGATTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAATTGTGACGCCACCACCGATAGTAATCTCACTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTTTCCATGCATAACTTCAACTTTCAgaaattgaaagcagaattcc                                        >  2:242034/1‑232 (MQ=14)                                                                                                                                                                                                                gtggtgAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGc                                    >  1:370283/1‑249 (MQ=255)                                                                                                                                                                                                                  ggtgAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAATGTGTTCTCCATGGATACATTCCCCTTTCAGCGAATGAAAGAAGCTTTCCACCCAGGc                                >  2:2886/1‑251 (MQ=255)                                                                                                                                                                                                                  ggtgAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGc                                <  1:78633/251‑1 (MQ=255)                                                                                                                                                                                                                           ttGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGt                        >  1:333880/1‑250 (MQ=255)                                                                                                                                                                                                                                     cccaaaaatcGGCATTTTGATCCACCCCACGAAATTTGTACACAGGCCTAAGCGAGGTTCTTGTTTTAAAATTGTTCCGGATTGGGGCCGCCACACCAACTGTGCCGCCGCCTCCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAAcc              <  2:129489/242‑1 (MQ=11)                                                                                                                                                                                                                                       cATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGCATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCtt            >  1:210812/1‑251 (MQ=25)                                                                                                                                                                                                                                                  aTTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTgcgtccat  >  2:258025/1‑245 (MQ=14)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AAAGCTTTAGCGGAAAGGATTCGGGAAAATGTTGAGCGTTTAACTGGCGATAATCCTGAATACGCTATACCACAAAAAGTGACGATTAGTATTGGCGCTGTTGTGACTCAGGAAAATGCGTTAAACCCAAATGAGATCTATCGACTGGCTGATAACGCACTTTACGAGGCGAAAGAGACCGGGCGTAATAAGGTGGTTGTGAGGGATGTGGTGAATTTTTGTG‑AGTCACCATAAAGCGGCATTTTGATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCCCGCATTATTTCCCGGCTGATAAAGTGTTCTCCATGGATACATTCCACTTTCAGCGAATGAAAGAAGCTTTCCACGCAGGCATTATCGTAGCAGCAACCTTTTGCGCTCAT  >  NC_000913/1094001‑1094490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.