Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,093,346	(T)6→5	coding (471/1359 nt)	dgcT →	putative diguanylate cyclase DgcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,093,346	0	T	.	100.0%	41.9 / NA	10	coding (471/1359 nt)	dgcT	putative diguanylate cyclase DgcT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/3);  total (7/3)

TTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTG  >  NC_000913/1093116‑1093589                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    tttCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGtt                                                                                                                                                                                                                                >  1:260100/1‑251 (MQ=255)                   tACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGt                                                                                                                                                                                                              >  2:357858/1‑251 (MQ=255)                                ccGTCATTATCATTCAGCAAAGTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCt                                                                                                                                                                                                 <  1:46098/251‑1 (MQ=255)                                          cATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTac                                                                                                                                                                                        >  1:15796/1‑250 (MQ=255)                                                                                     cAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGaaa                                                                                                                                            <  1:357858/251‑1 (MQ=255)                                                                                                      cTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACCCATTGCCGAGTTAACCAATGAAAATGGTCAGGTGTTTTGGAAAGCCTCATATGTTACCAt                                                                                                                           >  2:289030/1‑251 (MQ=255)                                                                                                                                                           tGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGtt                                                                      >  2:35978/1‑251 (MQ=255)                                                                                                                                                                                             tttatttTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGAGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGAt                                    >  2:87327/1‑251 (MQ=255)                                                                                                                                                                                                             aTGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATg                                                                                                                                                                                                       >  1:124500/1‑72 (MQ=255)                                                                                                                                                                                                             aTGACTTTGTGCATAAGTTTGTTTTT‑GTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATg                                                                                                                                                                                                       <  2:124500/72‑1 (MQ=255)                                                                                                                                                                                                                                 tgtgtttgtttttgGGGGGCCGATAGTTGCTCAAATACTTTCTAGTAATTATGAGAGCTACAATTTACACATTGCCGAGTTATCCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTATCATAATTATTTTCATGTGGTTAACCTTGCTTTCGGTGAATCTATTCTGTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTg  <  2:15796/246‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    TTTCTGAGTTGTTTGATTTACTTTGTTATAACCGTCATTATCATTCAGCAAATTATTGAGGAGCGTTTGACAAGCAGTGTTGTCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTGCATAAGTTTGTTTTTTGTTTTTGGGGGGCCGATAGTTGCTCACATACTTTCTAGTCATTATGAGAGCTACAATTTACACATTGCCGAGTTAACCAATGAAAATGGTCAGGTGGTTTGGAAAGCCTCATATGTTACCATAATGATTTTCATGTGGTTAACCTTGCTTTCGGTTAATCTATACTTTAATGGTTTACGCTATGATATCTGGAATGGAGTGACGGTGATTGCTTTTTGTGCCGTACTGTACAATATCTCTCTG  >  NC_000913/1093116‑1093589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑