Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,729,4400CT97.5% 115.4 / ‑4.3 40noncoding (106/160 nt)xylZsmall RNA XylZ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (22/17);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01
*NC_0009133,729,4410CT97.4% 118.3 / ‑4.1 40noncoding (105/160 nt)xylZsmall RNA XylZ
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (21/17);  total (23/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATC  >  NC_000913/3729202‑3729689
                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                        
ttCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCACCGGACTGCACAGTTAGTTGTTATTTGTCg                                                                                                                                                                                                                                               >  1:229365/1‑251 (MQ=255)
tgcATTGCGCGAACAGTTGCCTGCCACCACTGGTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTc                                                                                                                                                                                                                                                <  2:9063/248‑1 (MQ=255)
  cATTGCGCGAGCAGTTGCCTGCCACCACGGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCTAACGGACTGCACAGTTAGTTGTTATTTGTCGaa                                                                                                                                                                                                                                             <  1:409718/251‑1 (MQ=255)
   aTTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCGTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATGGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAAc                                                                                                                                                                                                                                            <  1:491533/251‑1 (MQ=255)
         cgATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGTCCAACGGACTGCACCGTTAGTTGTTATTTGTCGAACAGATa                                                                                                                                                                                                                                       >  2:400371/1‑250 (MQ=255)
                   cctgcgACCACTGTTCCGGGTCTTGTGCCGCCCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTACGCAGCAACCACCGCTCCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCa                                                                                                                                                                                                                            <  1:12323/251‑1 (MQ=255)
                      gccACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGAt                                                                                                                                                                                                                         <  1:400371/251‑1 (MQ=255)
                        ccccacTGTGCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGAttt                                                                                                                                                                                                                       <  2:275118/249‑1 (MQ=255)
                        caccacTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGAttt                                                                                                                                                                                                                       >  1:503052/1‑251 (MQ=255)
                               ggtccggggtttggtCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgtt                                                                                                                                                                                                                <  2:466926/237‑1 (MQ=255)
                                          tGTTCCGACCAGAGTGGATGCGGGTGCGAAACGTTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTGCCAGTTGTTCCTGGCg                                                                                                                                                                                                      <  2:105836/250‑1 (MQ=255)
                                                  ccAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg                                                                                                                                                                                             >  1:239270/1‑251 (MQ=255)
                                                         tgATGCGGGCGCGAAACGGTAAGCTTTTCCGTTTGCGCAGCAACCAACTCACCCTGCTCGTTGAGCATAATAACTTGTACGACCGAGGTGCCAAGATCTATCCCGATATACAGAACGATCGTTCCTTAAAAAAATGCCCGGTAGCGCTACCGATAACCGGGCCAACTGACTTCACATTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCTTGGCGCCCACTCTGATGctc                                                                                                                                                                                       <  2:383926/249‑3 (MQ=255)
                                                           aTGCGAGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGAGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGGTAATGGTTTACCATATTTTCCAGTTGTTCTTGTCGCCCACTCTGATGCACCgg                                                                                                                                                                                    >  2:316836/1‑251 (MQ=255)
                                                               gggCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGAc                                                                                                                                                                                <  1:102907/251‑1 (MQ=255)
                                                                     gAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAatg                                                                                                                                                                           >  2:102732/1‑250 (MQ=255)
                                                                         cGGTCAGCTTTTCCGTTTGCGCCGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGCGGTGCCAAGCTCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGtt                                                                                                                                                                      >  2:284154/1‑251 (MQ=255)
                                                                                             gcagcaACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTaaa                                                                                                                                                  <  1:36939/251‑1 (MQ=255)
                                                                                                       ccTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTg                                                                                                                                        <  1:102732/251‑1 (MQ=255)
                                                                                                                        aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCACGCTATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTACTGGCGACCACTCTGATGCACCGGAGACAAATGCTGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAgg                                                                                                                       >  1:390879/1‑251 (MQ=255)
                                                                                                                                    tttactcccgtgtggcccacaTCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTGCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATATTGGTTTGCCAGATTTTCCCGTTGTTCCTGTCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAAGCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGcc                                                                                                            <  2:303944/230‑1 (MQ=255)
                                                                                                                                             gAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCt                                                                                                   >  1:167871/1‑250 (MQ=255)
                                                                                                                                                            aTCCCGATATACATATCGATCGTTCCTTAAAAAAATACCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATa                                                                                    >  2:262972/1‑250 (MQ=255)
                                                                                                                                                              cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGCTAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGACCAGATCATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCTGAGACAAATGATTTTCCTGAACATATTTGGCTAAATCTGCCCGTGACATTTGGCCTTTCAGGCTTTGCTGGCCCCATTCGCTTTTCCAGCCTGAATAAcc                                                                                 >  2:256766/1‑250 (MQ=255)
                                                                                                                                                              cccGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAAc                                                                                  <  2:122821/250‑1 (MQ=255)
                                                                                                                                                                 gATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTg                                                                              <  2:258823/251‑1 (MQ=255)
                                                                                                                                                                   tataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGTGCCAACGGACTGCACAGTTAGTTGTTATTTGTAGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTACTGGCGGCCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGACAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTgcg                                                                            >  1:116135/1‑251 (MQ=255)
                                                                                                                                                                    ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTTTTATTTGTCGAACAGATAATGGTTTCCCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGCTGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGCTTTGCTGGCCCAATTCGCTATTCCAGCCGGACTACCGCTgcgc                                                                           >  2:53561/1‑251 (MQ=255)
                                                                                                                                                                       cATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGcgcggt                                                                        >  1:334033/1‑249 (MQ=255)
                                                                                                                                                                        atatCGAGCGTTCCTTAAACAACTGCCCGGTATCGCTACCGATAAACGGCCCAACGGCATACACAGTTAGTAGTTATTTGTCGAACAGCTAATGGTTTACCAGCTTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCCGTGACATTTGGCCTTTCAGGATTTGCTGGCCCACTTCGCAATTCCACCCGGAATAACACTgcg                                                                            >  2:244735/1‑246 (MQ=255)
                                                                                                                                                                            cgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGtt                                                                   >  1:190089/1‑251 (MQ=255)
                                                                                                                                                                                cgTTCCGTAAAAAAATGCCCGGTATCTCTACCGATAACCGGGCCAACGGACTGCACAATTAGTTGTTATTTGTCGAACAGATAATGGTTGACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTAGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTac                                                                <  2:339018/250‑2 (MQ=255)
                                                                                                                                                                                               tGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTc                                                >  2:495117/1‑251 (MQ=255)
                                                                                                                                                                                                             aCCGATAACCGGGCCAACGGACTGCACAGTTAGTTTTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTg                                  <  2:503052/251‑1 (MQ=255)
                                                                                                                                                                                                               cGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGc                                 >  1:207232/1‑250 (MQ=255)
                                                                                                                                                                                                               cGATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGc                                 >  1:8766/1‑250 (MQ=255)
                                                                                                                                                                                                                gATAACCGGGCCAACGGACTGCACAGTTAGTTGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCa                                >  2:8003/1‑250 (MQ=255)
                                                                                                                                                                                                                          ccAACGGCCTGCACAGTTAGTTGATATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTACTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATTCGTTTATCCAGCTCGCCATCTTCACTCATACGCGCTGAAATTTTCAgcgc                     >  2:331045/1‑251 (MQ=255)
                                                                                                                                                                                                                                gACTGCACAGTTAGTTGTTATTTGTCGAACAGATACTGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGc               >  2:476558/1‑251 (MQ=255)
                                                                                                                                                                                                                                             gCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATc  >  1:150053/1‑251 (MQ=255)
                                                                                                                                                                                                                                              ||                                                                                                                                                                                                                                                        
TTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAACCACCTCACCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCACCGGAGACAAATGATGTTCCTGAGCATATTTGGCTAAATCTGCCAGTGACATTTGGCCTTTCAGGATTTGCTGGCCCAATTCGCTATTCCAGCCGGAATAACGCTGCGCGATGCGTTTATCCAGCTCGCCATCTTCAATCATGCGCGCTGCAATTTTCAGCGCCAGTGCCATCGTATCCATC  >  NC_000913/3729202‑3729689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑