Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,618	T→C	Q114Q (CAA→CAG)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,618	0	T	C	97.1%	99.0 / NA	34	Q114Q (CAA→CAG)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (12/21);  minor base A (1/0);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

AGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTA  >  NC_000913/7378‑7845                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     aGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCCTTGGCATCCCGTTCTTTATAAAGATGTGcgcggg                                                                                                                                                                                                                            >  2:376138/1‑246 (MQ=255)    gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgagggaa                                                                                                                                                                                                                          <  1:148717/246‑8 (MQ=255)    gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCGGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAGCGGCGTTCGCCTGAAGTCCGCGGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCCTTATAAAGCTGTGCGAGGGAA‑c                                                                                                                                                                                                                        <  2:470132/247‑1 (MQ=255)     atcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑c                                                                                                                                                                                                                        >  2:270619/4‑250 (MQ=255)       cgCCAGCAGACCAAAGACAGCGAGAATAATTCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGTTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCAGCCCCATCCC‑GCGCTCCATATACCATGCCGTTCCGCCATGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CAtt                                                                                                                                                                                                                     <  1:257428/250‑1 (MQ=255)        gCCAGCAGAGCAAAGACAGCGAGAAAAATCCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTc                                                                                                                                                                                                                    <  2:95737/251‑1 (MQ=255)                   aaaGACAGGGAGAATAATACCTGTCACAAGCGGGGACCAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAACAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACTAAACTGACCATTGGCATGCCGTTCTTTCTAAAGCTGTGCGAGGTAA‑CATTCGGCAAACgcgg                                                                                                                                                                                                         >  2:49372/1‑248 (MQ=255)                    aaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAAATCAGGGCTCGCGCAACCGCGTTCGCCTGAATTACGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTTCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CCTTCGGCAAACgcggg                                                                                                                                                                                                        >  2:439216/1‑247 (MQ=255)                         aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGGCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCa                                                                                                                                                                                                   <  1:318570/251‑1 (MQ=255)                         aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCa                                                                                                                                                                                                   <  1:505054/251‑1 (MQ=255)                              gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGCCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGTAA‑CATTCTGCAACCGAGGTCGCCATGCCg                                                                                                                                                                                              >  1:332966/1‑251 (MQ=255)                                ataataCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGAt                                                                                                                                                                                            >  1:359529/1‑251 (MQ=255)                                                      aaaaaTCAAAAGAAAAACTCAGGGCCCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGATGTCGCCATGCCGATAAACGCGGCAACCCACATCCag                                                                                                                                                                      <  1:178893/250‑2 (MQ=255)                                                            cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                                                >  1:68946/1‑251 (MQ=255)                                                                agaaaaacacaggcctcgctcAACGGCGTTCGCCTGAATTCCGCTGAAAATTAAGCCGAAGGCGAGAAGCAAAAAGACGGCGAACAGAACGCCCACCCAGCGCACCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTc                                                                                                                                                            <  2:10144/231‑1 (MQ=255)                                                                  aaaaaCTCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCTGAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTcc                                                                                                                                                           >  1:365665/1‑250 (MQ=255)                                                                        ccAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCa                                                                                                                                                    <  1:308279/250‑1 (MQ=255)                                                                             gctcgccctaCGGCGTTCGCCTGAATCCCGCTGTAAATTATGCCGGAGGCGATGAGAAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCAAGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATACGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggc                                                                                                                                               <  2:391944/242‑1 (MQ=255)                                                                                        ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCa                                                                                                                                    <  1:69619/251‑1 (MQ=255)                                                                                             tCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcggacaatgccagccc                                                                                                                                >  2:68543/1‑237 (MQ=255)                                                                                                   gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGcc                                                                                                                          <  1:270619/246‑1 (MQ=255)                                                                                                             gAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                                 <  1:86650/249‑1 (MQ=255)                                                                                                                    agcccgtgggCGATGAGCAAAAAGACGGCGAACCGAACGCCCCTCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCATCGCAACGCCGGCCAGGTTGCCGCTAc                                                                                                         <  2:203242/242‑1 (MQ=255)                                                                                                                       ccgtAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                                     <  1:378756/248‑1 (MQ=255)                                                                                                                            ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGGAACGCCGGCCAGGTTGCCGCTAACCAcgcgcc                                                                                                >  1:374532/1‑250 (MQ=255)                                                                                                                                   aGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCa                                                                                           >  1:189626/1‑249 (MQ=255)                                                                                                                                         aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGt                                                                                   <  1:42738/251‑1 (MQ=255)                                                                                                                                                 ccaacagaccccccaccccacgctCCCCAGCCCTGGGCGCCATCTACCAGACCGGTTCGACACGAACCGGCCCATTGGCAGCACGTTCCTTAGAATGCTGTGCGAAGGAA‑CATCTGGCAAACGAGTCCCCCCTGCCGATAAACGCGGCAACCCACAGCCAGAAGCCGGCTCCAGGCCCACCGGCGGTAACTGCCAGCGCACCGCCGGCAAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACCCaac                                                                            <  2:58785/228‑2 (MQ=255)                                                                                                                                                                                     gcgcAATATCCCATGCCGTTCCGCCACGAACCGGCCTATTGCAATCCCTTTCTTTAAAAAGCTGTGCGAGGGAA‑ACTCCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCCACCCCCACCCAGAAGCCGGCTCCAGGACCACCGGCGGTAATTCCCAGCGCAACTCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGATTGAAATGAGGTTAAACCGCCTGGCTGTGGATGa                                        <  2:121880/251‑1 (MQ=255)                                                                                                                                                                                        caaattcccaggcgggccctccACAAAACTGCCCATTGACTAAACTTTCTTTATAAAGCT‑TGGGAGGGAAACTTTCGGCAAGCGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACTGCTCCAGTGCCACCGGCGGGAATTGCCGGCGCAACGCGGGCCAGGTGGCTGCTGCCCACGCGCGCCGCAAGACTGGGACAGAATGCCAGAAAGGAGTTTAAACCGCCTGTCTGTGGATGAATg                                     <  2:67471/231‑1 (MQ=255)                                                                                                                                                                                               ccATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAC‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAGCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAAGGACTGAAATGATGTTAAACCTCCTGGCTGTGGATGAATGCTAtttt                              <  1:141531/251‑1 (MQ=255)                                                                                                                                                                                                       gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCCACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTAAAAAATGACTGAAATGAGGGTTAACCGCCTGGGTGGGGGTGGATACTATTTTTAAGaatt                      >  1:337019/1‑248 (MQ=255)                                                                                                                                                                                                                            tggcatCACGTTCTTTATAAAGCTGTGCGAGTGAA‑CATTCGGCAACCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCCCCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTTTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTa  <  1:146370/245‑1 (MQ=255)                                                                                                                                                                                                                                           tataAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGGACACAATGGCTGAAATGAGGGTAAAACGCCTGGCTGTGGATGaa                                       >  1:420259/1‑198 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     AGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTA  >  NC_000913/7378‑7845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑