Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,600	G→A	D120D (GAC→GAT)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,600	0	G	A	97.1%	92.1 / NA	34	D120D (GAC→GAT)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/20);  minor base T (0/1);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTT  >  NC_000913/7355‑7825                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   cGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACg                                                                                                                                                                                                                               >  2:376738/1‑251 (MQ=255)   ggCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGtt                                                                                                                                                                                                                             <  1:328017/251‑1 (MQ=255)                        aGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCCTTGGCATCCCGTTCTTTATAAAGATGTGcgcggg                                                                                                                                                                                                        >  2:376138/1‑246 (MQ=255)                           gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgagggaa                                                                                                                                                                                                      <  1:148717/246‑8 (MQ=255)                           gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCGGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAGCGGCGTTCGCCTGAAGTCCGCGGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCCTTATAAAGCTGTGCGAGGGAA‑c                                                                                                                                                                                                    <  2:470132/247‑1 (MQ=255)                            atcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑c                                                                                                                                                                                                    >  2:270619/4‑250 (MQ=255)                              cgCCAGCAGACCAAAGACAGCGAGAATAATTCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGTTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCAGCCCCATCCC‑GCGCTCCATATACCATGCCGTTCCGCCATGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CAtt                                                                                                                                                                                                 <  1:257428/250‑1 (MQ=255)                               gCCAGCAGAGCAAAGACAGCGAGAAAAATCCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTc                                                                                                                                                                                                <  2:95737/251‑1 (MQ=255)                                          aaaGACAGGGAGAATAATACCTGTCACAAGCGGGGACCAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAACAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACTAAACTGACCATTGGCATGCCGTTCTTTCTAAAGCTGTGCGAGGTAA‑CATTCGGCAAACgcgg                                                                                                                                                                                     >  2:49372/1‑248 (MQ=255)                                           aaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAAATCAGGGCTCGCGCAACCGCGTTCGCCTGAATTACGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTTCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CCTTCGGCAAACgcggg                                                                                                                                                                                    >  2:439216/1‑247 (MQ=255)                                                aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGGCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCa                                                                                                                                                                               <  1:318570/251‑1 (MQ=255)                                                aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCa                                                                                                                                                                               <  1:505054/251‑1 (MQ=255)                                                     gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGCCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGTAA‑CATTCTGCAACCGAGGTCGCCATGCCg                                                                                                                                                                          >  1:332966/1‑251 (MQ=255)                                                       ataataCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGAt                                                                                                                                                                        >  1:359529/1‑251 (MQ=255)                                                                             aaaaaTCAAAAGAAAAACTCAGGGCCCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGATGTCGCCATGCCGATAAACGCGGCAACCCACATCCag                                                                                                                                                  <  1:178893/250‑2 (MQ=255)                                                                                   cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                            >  1:68946/1‑251 (MQ=255)                                                                                       agaaaaacacaggcctcgctcAACGGCGTTCGCCTGAATTCCGCTGAAAATTAAGCCGAAGGCGAGAAGCAAAAAGACGGCGAACAGAACGCCCACCCAGCGCACCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTc                                                                                                                                        <  2:10144/231‑1 (MQ=255)                                                                                         aaaaaCTCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCTGAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTcc                                                                                                                                       >  1:365665/1‑250 (MQ=255)                                                                                               ccAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCa                                                                                                                                <  1:308279/250‑1 (MQ=255)                                                                                                    gctcgccctaCGGCGTTCGCCTGAATCCCGCTGTAAATTATGCCGGAGGCGATGAGAAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCAAGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATACGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggc                                                                                                                           <  2:391944/242‑1 (MQ=255)                                                                                                               ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCa                                                                                                                <  1:69619/251‑1 (MQ=255)                                                                                                                    tCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcggacaatgccagccc                                                                                                            >  2:68543/1‑237 (MQ=255)                                                                                                                          gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGcc                                                                                                      <  1:270619/246‑1 (MQ=255)                                                                                                                                    gAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                             <  1:86650/249‑1 (MQ=255)                                                                                                                                           agcccgtgggCGATGAGCAAAAAGACGGCGAACCGAACGCCCCTCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCATCGCAACGCCGGCCAGGTTGCCGCTAc                                                                                     <  2:203242/242‑1 (MQ=255)                                                                                                                                              ccgtAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                 <  1:378756/248‑1 (MQ=255)                                                                                                                                                   ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGGAACGCCGGCCAGGTTGCCGCTAACCAcgcgcc                                                                            >  1:374532/1‑250 (MQ=255)                                                                                                                                                          aGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCa                                                                       >  1:189626/1‑249 (MQ=255)                                                                                                                                                                aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGt                                                               <  1:42738/251‑1 (MQ=255)                                                                                                                                                                        ccaacagaccccccaccccacgctCCCCAGCCCTGGGCGCCATCTACCAGACCGGTTCGACACGAACCGGCCCATTGGCAGCACGTTCCTTAGAATGCTGTGCGAAGGAA‑CATCTGGCAAACGAGTCCCCCCTGCCGATAAACGCGGCAACCCACAGCCAGAAGCCGGCTCCAGGCCCACCGGCGGTAACTGCCAGCGCACCGCCGGCAAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACCCaac                                                        <  2:58785/228‑2 (MQ=255)                                                                                                                                                                                                            gcgcAATATCCCATGCCGTTCCGCCACGAACCGGCCTATTGCAATCCCTTTCTTTAAAAAGCTGTGCGAGGGAA‑ACTCCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCCACCCCCACCCAGAAGCCGGCTCCAGGACCACCGGCGGTAATTCCCAGCGCAACTCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGATTGAAATGAGGTTAAACCGCCTGGCTGTGGATGa                    <  2:121880/251‑1 (MQ=255)                                                                                                                                                                                                               caaattcccaggcgggccctccACAAAACTGCCCATTGACTAAACTTTCTTTATAAAGCT‑TGGGAGGGAAACTTTCGGCAAGCGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACTGCTCCAGTGCCACCGGCGGGAATTGCCGGCGCAACGCGGGCCAGGTGGCTGCTGCCCACGCGCGCCGCAAGACTGGGACAGAATGCCAGAAAGGAGTTTAAACCGCCTGTCTGTGGATGAATg                 <  2:67471/231‑1 (MQ=255)                                                                                                                                                                                                                      ccATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAC‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAGCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAAGGACTGAAATGATGTTAAACCTCCTGGCTGTGGATGAATGCTAtttt          <  1:141531/251‑1 (MQ=255)                                                                                                                                                                                                                              gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCCACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTAAAAAATGACTGAAATGAGGGTTAACCGCCTGGGTGGGGGTGGATACTATTTTTAAGaatt  >  1:337019/1‑248 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTT  >  NC_000913/7355‑7825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑