Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,598 A→G V121A (GTC→GCC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,5980AG96.9% 91.5 / ‑6.0 34V121A (GTC→GCC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (13/18);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTT  >  NC_000913/7355‑7825
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    
cGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACg                                                                                                                                                                                                                               >  2:376738/1‑251 (MQ=255)
  ggCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGtt                                                                                                                                                                                                                             <  1:328017/251‑1 (MQ=255)
                       aGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCCTTGGCATCCCGTTCTTTATAAAGATGTGcgcggg                                                                                                                                                                                                        >  2:376138/1‑246 (MQ=255)
                          gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgagggaa                                                                                                                                                                                                      <  1:148717/246‑8 (MQ=255)
                          gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCGGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAGCGGCGTTCGCCTGAAGTCCGCGGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCCTTATAAAGCTGTGCGAGGGAA‑c                                                                                                                                                                                                    <  2:470132/247‑1 (MQ=255)
                           atcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑c                                                                                                                                                                                                    >  2:270619/4‑250 (MQ=255)
                             cgCCAGCAGACCAAAGACAGCGAGAATAATTCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGTTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCAGCCCCATCCC‑GCGCTCCATATACCATGCCGTTCCGCCATGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CAtt                                                                                                                                                                                                 <  1:257428/250‑1 (MQ=255)
                              gCCAGCAGAGCAAAGACAGCGAGAAAAATCCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTc                                                                                                                                                                                                <  2:95737/251‑1 (MQ=255)
                                         aaaGACAGGGAGAATAATACCTGTCACAAGCGGGGACCAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAACAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACTAAACTGACCATTGGCATGCCGTTCTTTCTAAAGCTGTGCGAGGTAA‑CATTCGGCAAACgcgg                                                                                                                                                                                     >  2:49372/1‑248 (MQ=255)
                                          aaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAAATCAGGGCTCGCGCAACCGCGTTCGCCTGAATTACGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTTCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CCTTCGGCAAACgcggg                                                                                                                                                                                    >  2:439216/1‑247 (MQ=255)
                                               aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGGCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCa                                                                                                                                                                               <  1:318570/251‑1 (MQ=255)
                                               aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCa                                                                                                                                                                               <  1:505054/251‑1 (MQ=255)
                                                    gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGCCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGTAA‑CATTCTGCAACCGAGGTCGCCATGCCg                                                                                                                                                                          >  1:332966/1‑251 (MQ=255)
                                                      ataataCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGAt                                                                                                                                                                        >  1:359529/1‑251 (MQ=255)
                                                                            aaaaaTCAAAAGAAAAACTCAGGGCCCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGATGTCGCCATGCCGATAAACGCGGCAACCCACATCCag                                                                                                                                                  <  1:178893/250‑2 (MQ=255)
                                                                                  cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                            >  1:68946/1‑251 (MQ=255)
                                                                                      agaaaaacacaggcctcgctcAACGGCGTTCGCCTGAATTCCGCTGAAAATTAAGCCGAAGGCGAGAAGCAAAAAGACGGCGAACAGAACGCCCACCCAGCGCACCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTc                                                                                                                                        <  2:10144/231‑1 (MQ=255)
                                                                                        aaaaaCTCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCTGAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTcc                                                                                                                                       >  1:365665/1‑250 (MQ=255)
                                                                                              ccAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCa                                                                                                                                <  1:308279/250‑1 (MQ=255)
                                                                                                   gctcgccctaCGGCGTTCGCCTGAATCCCGCTGTAAATTATGCCGGAGGCGATGAGAAAAAAGACGGCTAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCAAGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATACGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggc                                                                                                                           <  2:391944/242‑1 (MQ=255)
                                                                                                              ggCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCa                                                                                                                <  1:69619/251‑1 (MQ=255)
                                                                                                                   tCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcggacaatgccagccc                                                                                                            >  2:68543/1‑237 (MQ=255)
                                                                                                                         gaattCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGcc                                                                                                      <  1:270619/246‑1 (MQ=255)
                                                                                                                                   gAAAATTATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                             <  1:86650/249‑1 (MQ=255)
                                                                                                                                          agcccgtgggCGATGAGCAAAAAGACGGCGAACCGAACGCCCCTCCAGCGCATCCCCAGCCC‑GCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCATCGCAACGCCGGCCAGGTTGCCGCTAc                                                                                     <  2:203242/242‑1 (MQ=255)
                                                                                                                                             ccgtAGGCGATGAGCAAAAAGACGGCGAACAGACCGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCAc                                                                                 <  1:378756/248‑1 (MQ=255)
                                                                                                                                                  ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGGAACGCCGGCCAGGTTGCCGCTAACCAcgcgcc                                                                            >  1:374532/1‑250 (MQ=255)
                                                                                                                                                         aGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCa                                                                       >  1:189626/1‑249 (MQ=255)
                                                                                                                                                               aaGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGt                                                               <  1:42738/251‑1 (MQ=255)
                                                                                                                                                                       ccaacagaccccccaccccacgctCCCCAGCCCTGGGCGCCATCTACCAGACCGGTTCGACACGAACCGGCCCATTGGCAGCACGTTCCTTAGAATGCTGTGCGAAGGAA‑CATCTGGCAAACGAGTCCCCCCTGCCGATAAACGCGGCAACCCACAGCCAGAAGCCGGCTCCAGGCCCACCGGCGGTAACTGCCAGCGCACCGCCGGCAAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACCCaac                                                        <  2:58785/228‑2 (MQ=255)
                                                                                                                                                                                                           gcgcAATATCCCATGCCGTTCCGCCACGAACCGGCCTATTGCAATCCCTTTCTTTAAAAAGCTGTGCGAGGGAA‑ACTCCGGCAAACGAGGCCGCCATGCCGATAAACGCGGCCACCCCCACCCAGAAGCCGGCTCCAGGACCACCGGCGGTAATTCCCAGCGCAACTCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGATTGAAATGAGGTTAAACCGCCTGGCTGTGGATGa                    <  2:121880/251‑1 (MQ=255)
                                                                                                                                                                                                              caaattcccaggcgggccctccACAAAACTGCCCATTGACTAAACTTTCTTTATAAAGCT‑TGGGAGGGAAACTTTCGGCAAGCGAGGTCGCCATGCCGATAAACGCGGCAACCCCCATCCAGAAGACTGCTCCAGTGCCACCGGCGGGAATTGCCGGCGCAACGCGGGCCAGGTGGCTGCTGCCCACGCGCGCCGCAAGACTGGGACAGAATGCCAGAAAGGAGTTTAAACCGCCTGTCTGTGGATGAATg                 <  2:67471/231‑1 (MQ=255)
                                                                                                                                                                                                                     ccATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCGGTGCGAGGGAC‑CATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACAGCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAAGGACTGAAATGATGTTAAACCTCCTGGCTGTGGATGAATGCTAtttt          <  1:141531/251‑1 (MQ=255)
                                                                                                                                                                                                                             gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCCACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTAAAAAATGACTGAAATGAGGGTTAACCGCCTGGGTGGGGGTGGATACTATTTTTAAGaatt  >  1:337019/1‑248 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                    
CGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCC‑GCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAA‑CATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTT  >  NC_000913/7355‑7825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑