Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,639,440 C→T T421T (ACC→ACT creD → putative inner membrane protein CreD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,639,4400CT93.1% 76.0 / ‑3.4 30T421T (ACC→ACTcreDputative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (15/12);  total (16/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

ATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTT  >  NC_000913/4639214‑4639679
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               
atatcGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGttt                                                                                                                                                                                                                         <  1:314011/247‑1 (MQ=255)
    cggtttttcttgtgCATGGCTAATCGTCTGTCTGTTTGGTGCGTTAATGAACGGTATTTATTTGCAGGCGGTAGTGAAAGGTTGGCGTAACAGCTTGTGGTTGTCCCTCGCGCTGTTTTTGCTGGATGTTGTGATGTGGGGACTGCTCATCTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCTGGCATGATGTTTGTGACTCGTAATATCGACTTGTATGCGGTTTCa                                                                                                                                                                                                                      <  2:476684/237‑1 (MQ=255)
                   aTGGCTAATCGCAAGGCTGAGTGGGGATTTAATGATCGGTTTTTATTTGCAGGCGGTATTGAAAGGCTGGCTCAACATCATGTTGTTTTCCCTCTCGCTGGTGTTGCTGGAGGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGTCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAg                                                                                                                                                                                                      <  2:406393/251‑1 (MQ=255)
                               cAGTCTGATTGGGGCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGGTGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCCGTGTGCTGGTGGTGGCGCTGGCCGGCATGGTGTTTTTGACTCGTAATATCGACTGGGATGCGTTTTCACTGCCGAAAATGAAAACCAGTaaaaaa                                                                                                                                                                                           >  2:185456/1‑247 (MQ=255)
                                         ggggCGTTAATGAACGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTAACCTCGCGCTGTTGTTGCTGGGTGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACaaaggg                                                                                                                                                                                >  1:371056/1‑247 (MQ=255)
                                                         ggatttatttGCAGTTGGGATTGAAAGGCGGGCGCAACATCATGTGGTTTACCCTCTCGCTTTTGGTGCTGGATGGTGTGAGTTGGGGACTGCTGAACTCTGCCGATAGCGCGCTGTTGTGGGGAACCATTTTGCTGGTGGTGGCGCTGGCCGGCATGATGGTTGTGACTGGTAAGATCGACTTGTATGCGTTTGTACGGCCGAAAATGAAAGCCAGTAAAGAAGTTACaatggtcgcgcaattacgtatc                                                                                                                                                                <  2:22394/249‑21 (MQ=255)
                                                         gTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGGGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCCTGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTAAGTATc                                                                                                                                                                >  1:390815/1‑251 (MQ=255)
                                                              tatttGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGa                                                                                                                                                            >  1:234528/1‑250 (MQ=255)
                                                                tttGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAAt                                                                                                                                                         >  1:246786/1‑251 (MQ=255)
                                                                   gcgtgtggTATTGAAAGGCGGGCGCAACAGCATGTTGTGATCCCTCGGGCTGTTGGTGCTGGAGGGTGTTATGTTGGGCCTGCTCAACTCGGCCGATAGCGAGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATAAAGACTGGTATGCGTTTTCACTGCCGAATATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATaa                                                                                                                                                       <  2:163111/244‑1 (MQ=255)
                                                                           tATTGACAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGGTCAATTACGTATCTGGAAATAAGGTTGaaa                                                                                                                                               >  2:114320/1‑250 (MQ=255)
                                                                                      tggcgCAACAGAATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGCCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTATCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGTACAATTACGTATATGGAAATAAGGTTGAAAAATAAAAACggg                                                                                                                                   >  2:50287/5‑250 (MQ=255)
                                                                                          gCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGCAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaaaa                                                                                                                                        >  2:426654/1‑242 (MQ=255)
                                                                                          gCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaaaa                                                                                                                                        <  1:426654/242‑1 (MQ=255)
                                                                                               ggCATGTTGTTTACCCACGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCGGCCGATAGCGCGCGGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGAATCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAg                                                                                                                          <  2:40980/250‑1 (MQ=255)
                                                                                                        tttACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtttt                                                                                                                 >  1:159240/1‑251 (MQ=255)
                                                                                                            cccTCGCGCTGTGGTGGATGGATGGTGTGATGTGGGGACTGCTCAACCCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGtttttttt                                                                                                             <  1:101359/251‑1 (MQ=255)
                                                                                                             cctcggtgagtttgtgttGGATGGGTTGAGGTGGTGACTGTGCAACTCTGCCGATTGCTGGCTGTTGTTGGGAACCAGGGTGTGTGTGGTGGTGTTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAAGTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGgttttttga                                                                                                            <  2:441729/234‑3 (MQ=255)
                                                                                                                     tgttgtTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTaaaaa                                                                                                                           >  1:353107/1‑228 (MQ=255)
                                                                                                                     tgttgtTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTaaaaa                                                                                                                           <  2:353107/228‑1 (MQ=255)
                                                                                                                            gCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCg                                                                                             >  1:158824/1‑251 (MQ=255)
                                                                                                                                       tgATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTcc                                                                                  >  2:97757/1‑251 (MQ=255)
                                                                                                                                               ggACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCAcc                                                                          >  2:245464/1‑251 (MQ=255)
                                                                                                                                                                  aTAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTc                                                        >  1:289328/1‑250 (MQ=255)
                                                                                                                                                                  aTAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGACACCCTTc                                                        >  2:370101/1‑250 (MQ=255)
                                                                                                                                                                                  gggAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTTGCTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAATTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGGTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCga                                       <  1:286148/251‑1 (MQ=255)
                                                                                                                                                                                        cAGTGTGGTGGGGGGGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGAttt                                 <  2:208651/251‑1 (MQ=255)
                                                                                                                                                                                                                     atggtgTTTGTGCCTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGTTCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAACCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGTTGGTGGCGATGATTTCCGGCGTATCCGGGGTAGATTCGAAATg     >  2:290076/5‑251 (MQ=255)
                                                                                                                                                                                                                     atgatgTTTGGGAAACATAATAACGAATGGGATGCGTTTTCACTGCCGAAAAGGAAAGCCAGTAAAGAAAGTAAAATGGACGATCAATTACGTAACTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATg     <  2:480687/251‑1 (MQ=255)
                                                                                                                                                                                                                        atgTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTGCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGttt  <  2:246786/251‑1 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               
ATACCGGTTTTACCGTGGCATGGATAATCGCCAGTCTGATTGGGGCGATAATGAACGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTT  >  NC_000913/4639214‑4639679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: