Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,005	G→C	P276P (CCG→CCC)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,005	0	G	C	97.4%	116.4 / ‑6.2	40	P276P (CCG→CCC)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (17/21);  minor base A (0/1);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCA  >  NC_000913/4638758‑4639246                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  aCCTGAAACTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAAATGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAATTTTGTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCcgcg                                                                                                                                                                                                                                                <  2:377795/251‑1 (MQ=255)                       gAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCCGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAACGCAGCTGGTTTGCTAATAATCTCTGTGGGCGGTTTGCTTCAGGCAATGATCCCGGCTGGGAAAAATTACCCGCGTTTAAGGTCGCAgagacaaccg                                                                                                                                                                                                                          >  1:269500/1‑242 (MQ=255)                         aTTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTGCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGGTTAGCGTCGCAGTGACGACGCCa                                                                                                                                                                                                                        <  1:378572/251‑1 (MQ=255)                             aaGCGGTCCCGGCGATCTTTCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGcc                                                                                                                                                                                                                     <  2:283157/250‑1 (MQ=255)                              aGCGGTCCCGGCGATCTTGCTGTGGTGCCGGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTGTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGa                                                                                                                                                                                                                   <  2:445126/251‑1 (MQ=255)                                  gttccGGCGATCTTTCTGGGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGGCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATTCCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCaa                                                                                                                                                                                                               <  1:418483/248‑1 (MQ=255)                                               ttCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTTGCGTCGCCGTGACGCCGGCAGCCGGTCAAttcacattaa                                                                                                                                                                                                     >  2:165486/1‑239 (MQ=255)                                               ttCTGTGGTGCCTGCCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTaa                                                                                                                                                                                                     <  1:165486/248‑1 (MQ=255)                                                    gggggCCTGCCGGGCGTAAAAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGATTCAGGTTTTCAGGCGCAATGGAAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGGGACGACGCCAGCCGAGCAATACCAATTAAcggtccgg                                                                                                                                                                                             <  2:367211/247‑8 (MQ=255)                                                                gggggaaaaGCGAAATGACCCTAACCAGCAACTGGGCGAATCCCAGGTTTTTAGGTGAATTTTTGCTAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAACGTCAAAGCAGCTGGTTTGCTGATAACCTCGGTGAGCGTTTTGTTTCGGGCAATGATACGGGCTGGGAAAACGTCCCCGCGTTTAGCGGCGCAGTGACGAGGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTAc                                                                                                                                                                                 <  2:45351/243‑1 (MQ=255)                                                                                        ccAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGa                                                                                                                                                           >  1:25410/1‑249 (MQ=255)                                                                                        ccAGCAACTGGCCGCAGCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGa                                                                                                                                                           <  2:25410/249‑1 (MQ=255)                                                                                                    cGCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGt                                                                                                                                                                                   >  1:355533/1‑213 (MQ=255)                                                                                                    cGCATCCCAGTTTTTTAGGTGAGTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTGCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATGCCAATGAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTc                                                                                                                                             <  1:223283/251‑1 (MQ=255)                                                                                                     gCATCCCAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTAAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGCCTTTTATGGCGTTCt                                                                                                                                            >  1:476684/1‑251 (MQ=255)                                                                                                         cccAGTTTTTTAGGTGATTTTTTGCCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGt                                                                                                                                        >  1:495646/1‑251 (MQ=255)                                                                                                                    aGGTGATTTTTTGCTAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAAGGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTATCGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACg                                                                                                                              <  1:465693/250‑1 (MQ=255)                                                                                                                     ggTGATTTTTTGCCAGCCACACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTGGCGTAGCCGTGGCGACGCCAGCCGATCCATACCAAATCACGGAACGGGCGAATTAATACCACACTTTGCTGATTTCACTGGCTTTTCTGGCGTTCTTTTTTTTTGAAAcacc                                                                                                                            >  2:61603/1‑248 (MQ=255)                                                                                                                      gtgagttgttgccAGCCAACCGGGAAGTTAGCGAGTCAGGTTTGCCGGCGCAAGGGCAAAGCAGCTGGTTGGTTAATAATTGCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTGGAAACGCTc                                                                                                                           <  2:149178/240‑1 (MQ=255)                                                                                                                               tgccAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGGCTAAGTACGCCCTTCTGCTGATTTCCATGGCTTTTATGGCGTTCTTTTTTTTTGAAACGCTCACCTCGCa                                                                                                                   >  2:229749/3‑250 (MQ=255)                                                                                                                                   aGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGtt                                                                                                              <  1:211303/251‑1 (MQ=255)                                                                                                                                             gAAGTTAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAAt                                                                                                    >  1:40980/1‑251 (MQ=255)                                                                                                                                                  tAGCGAGTCAGGTTTTCAGGCGCAATGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAat                                                                                               >  1:331507/1‑251 (MQ=255)                                                                                                                                                                 tCAGGCGTAATGGCAAAGCAGCTGGTTTGCTAATAATTTCGGTGGGCGTTTTGCTACAGGCAAGGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTgggg                                                                                 <  2:274168/250‑1 (MQ=255)                                                                                                                                                                      cgcAATGGCAAAGCAGCTGGTTTGCTACTAATCTCGGTGAGCGTTTTGCTTCAGGCACTGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCCTTCTGCTGATTGCACTGCCTTTTATGGCGTTCTTTGTTTTTTAAACGCTCACCGCGCAACGTTTAAACCCAATGCAATATTTGCTGGTGGGGCTTTc                                                                            >  1:163111/1‑250 (MQ=255)                                                                                                                                                                              cAAAGCAGCTGGTTTTCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCTCCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAt                                                                   <  1:242792/251‑1 (MQ=255)                                                                                                                                                                              cAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGAt                                                                   >  1:38320/1‑251 (MQ=255)                                                                                                                                                                               aaaGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATg                                                                  <  1:324496/251‑1 (MQ=255)                                                                                                                                                                                    agcTGGCTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAACCTTCCCCGCGTTTAGCGGCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTa                                                             <  1:266253/251‑1 (MQ=255)                                                                                                                                                                                        ggTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTAttt                                                          <  1:229749/250‑1 (MQ=255)                                                                                                                                                                                                aataatCGCGGTGGGCGTTTTTCTTCAGGCAAAGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGGCGCAGTGACGACCCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGc                                                  <  2:390238/250‑1 (MQ=255)                                                                                                                                                                                                  taatCTCGGTGAGCGTTGTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGCCGCCAGCCGATCAATACCAATTAACGGACCGCGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAAAGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTTATTGGTACTGTTTTATTTGCTCTTGATgg                                               >  2:101359/1‑251 (MQ=255)                                                                                                                                                                                                         ggTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCCAACCGCGCAACGTTTACACCCAATGCCATATTTGTTGGTGGGGCTTTCATTGGGGGTGTTTTATTTGCTCTTGCTGTCGCTTTc                                        >  2:314011/1‑251 (MQ=255)                                                                                                                                                                                                                   ttGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATat                               <  2:210857/250‑2 (MQ=255)                                                                                                                                                                                                                        tcaagCAATGATACCGGCTGGGAAAACTTCCCCCCGGTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCAtt                                                                                                                                                                           >  2:393248/5‑105 (MQ=255)                                                                                                                                                                                                                        tCAGGCAATGATACCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCAtt                                                                                                                                                                           <  1:393248/105‑1 (MQ=255)                                                                                                                                                                                                                              aaTGTTACCGGATGGGAAACCTCCCCCGCGTTTAGCGGCGCAGTGACGTCCCCAGCCGATCAATACCTATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGt                   <  2:382535/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCCGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGGGGGGCTTTCATTGGTGGTGTTTTATTTGCTCTTGATGGAGCTTTCTGAACATATCGGTTTTACCGTGGCATg             >  1:16395/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGGCTGGGAAAACTTCCCCGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATgg            >  1:416810/1‑250 (MQ=255)                                                                                                                                                                                                                                             gAAAACTTCCCCGCGTTTAGCGGCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGc    <  1:83155/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaaCTTCCCAGCGTTTAGCGTCGCAGTGACGACGCCAGCCGATCAATACCAATTAACGGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATATCGGTTTTACCGTGGCATGGATAATCGCCa  <  2:33660/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGTGGTGCCTGGCGGGCGTAATAGCGAAATGACCTTAACCAGCAACTGGCCGCATCCCAGTTTTTTAGGTGATTTTCTACCAGCCAAACGGGAAGTTAGCGAGTCAGGTTTTCAGGCGCACTGGCAAAGCAGCTGGTTTGCTAATAATCTCGGTGAGCGTTTTGCTTCAGGCAATGATACCGGCTGGGAAAACTTCCCGGCGTTTAGCGTCGCAGTAACGACGCCAGCCGATCAATACCAATTAACTGACCGGGCGACTAAGTACGCCATTCTGCTGATTGCACTGACTTTTATGGCGTTCTTTGTTTTTGAAACGCTCACCGCGCAACGTTTACACCCAATGCAATATTTGCTGGTGGGGCTTTCATTGGTGATGTTTTATTTGCTCTTGCTGGCGCTTTCTGAACATACCGGTTTTACCGTGGCATGGATAATCGCCA  >  NC_000913/4638758‑4639246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑