Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,459	G→C	A167A (GCC→GCG)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,459	0	G	C	100.0%	105.3 / NA	41	A167A (GCC→GCG)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/20);  total (21/20)

GCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCA  >  NC_000913/7217‑7699                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGc                                                                                                                                                                                                                                          >  2:86650/1‑251 (MQ=255)                cctaaaacACTCGCAAAAAGAGACCAAAGGACGGGGGGAAGTTGCCCGATATTCAGTACGCAAATCACCAGGCGGGGCAGTACCCAGAGTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTcc                                                                                                                                                                                                                           <  2:142974/244‑1 (MQ=255)                     aGCACTCTCAAAAATAGACCAACTGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGCTCGCCTGAATTCCGCTg                                                                                                                                                                                                                       >  1:355907/1‑250 (MQ=255)                                        cAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGCTTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGCAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGAAGGCGAt                                                                                                                                                                                                   >  2:505054/1‑251 (MQ=255)                                          aaTGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATg                                                                                                                                                                                                  >  2:148717/1‑250 (MQ=255)                                          aaTGACGGGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATGAGCGCCATCAACGGGACAAACCCCGGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCTCGCAACGGCGTTCGGCTGAATTCCGCTGAAAATTATGCCGTACGCGATGa                                                                                                                                                                                                 <  2:323018/251‑1 (MQ=255)                                                  ggggAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGATAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCaaaaa                                                                                                                                                                                          <  1:153228/250‑1 (MQ=255)                                                  ggggAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCaaaaa                                                                                                                                                                                          <  1:433510/250‑1 (MQ=255)                                                    ggAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGCTTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAACAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGAc                                                                                                                                                                                       >  1:205179/1‑251 (MQ=255)                                                     gAAGTTGCCCGATATTCATTACGAAAATCACCAGGCTGGTCAGTACACAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGGGATAGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTAACCAGCGGGGGAAAATCAAAAGAAAACCTCAGGGCACGCGCAACCACGATCCCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGACCCAAAAGACg                                                                                                                                                                                      >  2:363078/1‑251 (MQ=255)                                                     gAAGTTGCCCGATAATCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACg                                                                                                                                                                                      <  2:156562/251‑1 (MQ=255)                                                       aGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGc                                                                                                                                                                                    >  2:42738/1‑251 (MQ=255)                                                                                 cgccAGGCTGGTCAGTACCCAGAGTATCGCCATCAACGGGACAACCCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATcc                                                                                                                                                          <  2:38858/249‑1 (MQ=255)                                                                                     aGGCTGGTCAGTACCCAGATGATCGCCATCAACGGGACAAACCCCTGCATGAGCCGTGCGACGCCATGAAGACCGCGAGTGATCACCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGTGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGTCTGAATTCCGCTGAAAATTATGCCGTAGGCGAGGAGCAAAAAGGCGGCGAACAGAACGCCCATCCAGCGCATCCCCAg                                                                                                                                                      <  2:477669/251‑1 (MQ=255)                                                                                           gTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGACATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCcgcg                                                                                                                                                >  1:243338/1‑251 (MQ=255)                                                                                                   ccAGATTACCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCatat                                                                                                                                        <  1:310231/251‑1 (MQ=255)                                                                                                           tCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGcc                                                                                                                                >  2:378756/1‑251 (MQ=255)                                                                                                                  cAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCACATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTcc                                                                                                                           <  1:344785/249‑1 (MQ=255)                                                                                                                       ggacacaccccTGCATGAGCCGGGCGACGCCATGAAGACAGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGACACCAGCGGGGGAAAATCACAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGGGCAAAAAGACGGCGAACAGCACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACg                                                                                                                     >  2:497689/9‑250 (MQ=255)                                                                                                                         aCAAACCCCTGCATGAGCAGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAACGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGACCGCCCCGaa                                                                                                                   >  2:308279/1‑250 (MQ=255)                                                                                                                              ccccTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACTGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCTATATACCATGCCGGTCCGCCACGAAACTGcc                                                                                                             <  1:164531/250‑1 (MQ=255)                                                                                                                                     aTGAGCCGGGCGACGCCAGGAAGCCCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAAAAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTggc                                                                                                      <  2:273522/251‑3 (MQ=255)                                                                                                                                          ccGGGCGACGCCATGAAGACCGCGCGAGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTAACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAACTTATGCCGTAGGCGATGAGCAAAAAGACGGCGAAAAGAACGACCATCCAGCGCATCCCAAGCCCGCGCGCCATATACCATGCCGGTCCGACCCGAAACTGCCCATTggccgcac                                                                                                 >  2:199537/1‑244 (MQ=255)                                                                                                                                           cGGGCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGCAACTGCCCATTGGCATCACg                                                                                                >  2:376738/1‑251 (MQ=255)                                                                                                                                             ggCGACGCCATGAAGACCGCGAGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGtt                                                                                              <  1:328017/251‑1 (MQ=255)                                                                                                                                                                  aGTGATCGCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCCTTGGCATCCCGTTCTTTATAAAGATGTGcgcggg                                                                         >  2:376138/1‑246 (MQ=255)                                                                                                                                                                     gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGcgagggaa                                                                       <  1:148717/246‑8 (MQ=255)                                                                                                                                                                     gatcgCCAGCAGAGCAAAGACAGCGAGAATAATACCGGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAGCGGCGTTCGCCTGAAGTCCGCGGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCCTTATAAAGCTGTGCGAGGGAAc                                                                      <  2:470132/247‑1 (MQ=255)                                                                                                                                                                      atcgCCAGCAGAGCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAAc                                                                      >  2:270619/4‑250 (MQ=255)                                                                                                                                                                        cgCCAGCAGACCAAAGACAGCGAGAATAATTCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGTTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCAGCCCCATCCCGCGCTCCATATACCATGCCGTTCCGCCATGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACAtt                                                                   <  1:257428/250‑1 (MQ=255)                                                                                                                                                                         gCCAGCAGAGCAAAGACAGCGAGAAAAATCCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTc                                                                  <  2:95737/251‑1 (MQ=255)                                                                                                                                                                                    aaaGACAGGGAGAATAATACCTGTCACAAGCGGGGACCAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAACAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACTAAACTGACCATTGGCATGCCGTTCTTTCTAAAGCTGTGCGAGGTAACATTCGGCAAACgcgg                                                       >  2:49372/1‑248 (MQ=255)                                                                                                                                                                                     aaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAAATCAGGGCTCGCGCAACCGCGTTCGCCTGAATTACGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTTCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACCTTCGGCAAACgcggg                                                      >  2:439216/1‑247 (MQ=255)                                                                                                                                                                                          aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGGCGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCa                                                 <  1:318570/251‑1 (MQ=255)                                                                                                                                                                                          aGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCa                                                 <  1:505054/251‑1 (MQ=255)                                                                                                                                                                                               gaATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGCCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGTAACATTCTGCAACCGAGGTCGCCATGCCg                                            >  1:332966/1‑251 (MQ=255)                                                                                                                                                                                                 ataataCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGAt                                          >  1:359529/1‑251 (MQ=255)                                                                                                                                                                                                                       aaaaaTCAAAAGAAAAACTCAGGGCCCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGATGTCGCCATGCCGATAAACGCGGCAACCCACATCCag                    <  1:178893/250‑2 (MQ=255)                                                                                                                                                                                                                             cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg              >  1:68946/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaaaCTCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCTGAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTcc         >  1:365665/1‑250 (MQ=255)                                                                                                                                                                                                                                         ccAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCa  <  1:308279/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCA  >  NC_000913/7217‑7699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑