Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,637,675	C→T	A327V (GCT→GTT)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,637,675	0	C	T	100.0%	121.5 / NA	40	A327V (GCT→GTT)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/23);  total (17/23)

TGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGAT  >  NC_000913/4637432‑4637913                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               tGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGAt                                                                                                                                                                                                                                         >  1:479204/1‑251 (MQ=255)                    gAAAAACTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGGTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTAAGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGAtggggagggcttattttgcc                                                                                                                                                                                                                      >  2:107309/1‑233 (MQ=255)                          cTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatt                                                                                                                                                                                                                           <  2:457794/240‑1 (MQ=255)                          cTATATTGAGCAGTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCattatt                                                                                                                                                                                                                           >  1:457794/1‑240 (MQ=255)                             gattgagccggattgttaagcgttAACCCATGAGGTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGTAAATTTTACGCGAAGGGCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGGGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGc                                                                                                                                                                                                              <  2:208558/228‑1 (MQ=255)                                  agcagttggtttatgcggtAACCCATGAGCTAAATAGCCCACTTGCGGCGATTCGTTGCGCGGCGGAAATTTTACGCGAAGGTCCTCCACCGGAAGTGGTGGCTCGTGTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTGCTGACTTTTGGTGAAGTGGTGGCATTATATCGCCGCGTCAGCGAAgcg                                                                                                                                                                                                        <  2:362908/233‑1 (MQ=255)                                      gTATGTTTATGCGTTAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGGCGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGGGAATCGTAAGGAAGTCGTTCTGCCTGTTGTTGATGTGGCGGCATTATTTGGCCGCGGCACCGTAgcgcgcc                                                                                                                                                                                                    >  2:138834/1‑250 (MQ=255)                                          ggttatgcgttAACCCATGAGCTAAAAAGCCCACGGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCg                                                                                                                                                                                                 <  2:259985/241‑1 (MQ=255)                                           tttatgcgttAACCCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGGGAAgagggccccggg                                                                                                                                                                                               >  1:40221/9‑240 (MQ=255)                                                      acccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGGTCTGACTGTTGTTGATGTGGCGGCAATATTTCGCCGCGTCAGCGAAGCGCGCCCCGAGCAGTTGGcgg                                                                                                                                                                                     >  1:393973/4‑248 (MQ=255)                                                       cccATGAGCTAAAAAGCCCACTGGCGGCGTGTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTTCCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATGATTTCGCCGCGTCAGCGAATCGCGCACCTTGCAGTTGGCAGaa                                                                                                                                                                                   <  2:331663/249‑1 (MQ=255)                                                        ccATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaa                                                                                                                                                                                   >  1:343515/2‑250 (MQ=255)                                                             aGCTAAAAAGCCCACTGGCGGCGATTCGTTGCGCGGCGGAAATTTTACGCGAAGGGCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATGTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGaaaaaaac                                                                                                                                                                             <  2:251783/251‑2 (MQ=255)                                                                   aaaGCCCACTTGCGGCGAGTCGTGGCGTGGCGGAAATTTTACTCGAAGGGCCGCCACCGGAAGGGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGGGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGTCAGAAAAAAACATCAct                                                                                                                                                                       <  2:339571/251‑1 (MQ=255)                                                                        ccACTGGCGGCGATTCGTGGCGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGGAGTGGGGGCTCGTTTTACCGACAACATTCTGACGCAAAGTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGCTGTGGCGGCGATCTTTTGCCGCGTCCGCGAGCCGCGCCCCGTGCAGATGGCCGgaaaaaaccacaatctacc                                                                                                                                                                  >  2:132348/1‑239 (MQ=255)                                                                               cggcgCTTCGGGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGCCAACATTCTGACGAAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTACCTGTTGTTGCTGTGGCGGCATTATTTCGACGCGGCCGCGGAGAGCGACCCGGGCCGTTGGCAGAGAAAAAAATCAatctcacagttatg                                                                                                                                                           >  2:503816/1‑241 (MQ=255)                                                                                            cgcggcgGAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTa                                                                                                                                              <  1:412230/250‑1 (MQ=255)                                                                                                  ggAAATTTTACGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTtg                                                                                                                                        <  1:378929/251‑1 (MQ=255)                                                                                                         ttACGCGAAGGGTCGCCACCGAAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTTGAGAAGAGTCAGGAAGTGGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTTAGCGATGCGCGCACCGTGCAGGTGGCAGAAAAAAACAGCACTCGACATGTTATGCCCACTGAGGTTAATGTTGctgctg                                                                                                                                  <  2:288022/250‑1 (MQ=255)                                                                                                           aCGCGAAGGTCCGCCACCGGAAGTGGTGGCTCGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGTAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGaa                                                                                                                                <  1:205956/250‑1 (MQ=255)                                                                                                                                          cGTTTTACCGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAAttt                                                                                                <  2:238339/251‑1 (MQ=255)                                                                                                                                                            cTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACCTGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCg                                                                               >  2:192092/1‑250 (MQ=255)                                                                                                                                                               aCGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTATTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGGCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGAttt                                                                           <  1:400921/251‑1 (MQ=255)                                                                                                                                                                cGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGAtttt                                                                          >  1:376968/1‑251 (MQ=255)                                                                                                                                                                     aaTGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTAcccc                                                                     <  2:376968/251‑1 (MQ=255)                                                                                                                                                                            gTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGc                                                              <  1:358173/251‑1 (MQ=255)                                                                                                                                                                              aTGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTaatg                                                                                                                                           <  2:461836/172‑3 (MQ=255)                                                                                                                                                                                 cAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGtcg                                                         <  1:188987/251‑3 (MQ=255)                                                                                                                                                                                               aaCGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCCGTTGGCAGAAAAAAACATTAATCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCggacgcataaagcttagcg                                           >  1:310390/1‑234 (MQ=255)                                                                                                                                                                                                aCGTTACTACGCCAGGCAAGACTGGAGAAGCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGTACCGTGCAGTTGGCAGAAAAGAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCAGCGATTTTACCCCCGAGAGCGTTCGCATAACGCTAAgcgc                                          <  1:503816/251‑1 (MQ=255)                                                                                                                                                                                                        acGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGAATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGGTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTgg                                  >  1:246534/1‑251 (MQ=255)                                                                                                                                                                                                           ccAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATc                               <  1:496245/251‑1 (MQ=255)                                                                                                                                                                                                            cAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCa                              <  2:343515/251‑1 (MQ=255)                                                                                                                                                                                                                        gagaATCGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCCCTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTcgc                  >  2:29242/1‑249 (MQ=255)                                                                                                                                                                                                                          gaATCGACAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAACCATCACTCTACATGGTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGGTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGGCGCATAACGCTAAGCGCCGAAGGGGGTCAGGAACACGTcgccc                >  1:24738/1‑247 (MQ=255)                                                                                                                                                                                                                              cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTCTTTCGCCGCGTCAGCGCAGCGCGCACCGTGCAGTTGGCAGAAAAAAACCTCCCTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGGGCAGGCGCTGGGGAAATTACTGGGTAACGccctc                                                                                >  2:231594/1‑180 (MQ=255)                                                                                                                                                                                                                              cGTCAGGAAGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATc                                                                                <  1:231594/183‑1 (MQ=255)                                                                                                                                                                                                                                     aaGTCGTTCTGACTGTTGTTGATGTGGCGGCATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTg     <  2:407711/251‑1 (MQ=255)                                                                                                                                                                                                                                       gTCGTTCTGACTGTTGTTGATGTGGCGGCCTTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGGTGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTAATGGATAACGCTATCGTTTTTACCCCCGAGAGCGGTCGCATAACGCTTAGCGCCGGAGTGGTTCAGGacaacgtcgaccttcaggggctggg   >  2:299495/1‑227 (MQ=255)                                                                                                                                                                                                                                         cattCTTACTGTTGTTGATGTGGCGGAATTATTTCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTGGGCAGAAAAAAACATCACTCTACATGTTATGCCCACTGAGGTTAATGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGGAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTCGCATAACGCTAAGCGCCGAAGTGGATCAGGAACACGTCGCCCTTAAGGTGCTGGAt  <  2:365365/248‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                               TGCGCGTGAAGCTGGAAGGGAAAAACTATATTGAGCAGTATGTTTACGCATTAACTCATGAGCTAAAAAGCCCACTGGCGGCGATTCGTGGAGCGGCGGAAATTTTACGCGAAGGTCCGCCGCCGGAAGTGGTGGCTCGTTTTACTGACAACATTCTGACGCAAAATGCGCGTATGCAGGCATTGGTAGAAACGTTACTACGCCAGGCAAGACTGGAGAATCGTCAGGAAGTCGTTCTGACTGCTGTTGATGTGGCGGCATTATTCCGCCGCGTCAGCGAAGCGCGCACCGTGCAGTTGGCAGAAAAAAAAATCACTCTGCATGTTACGCCCACCGAGGTTAACGTTGCTGCTGAACCGGCGTTACTGGAGCAGGCGCTGGGAAATTTACTGGATAACGCCATCGATTTTACCCCCGAGAGCGGTTGCATCACGCTAAGCGCCGAAGTGGATCAGGAACACGTCACGCTTAAGGTGCTGGAT  >  NC_000913/4637432‑4637913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑