Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,636,591 C→T V195V (GTC→GTT)  creB → DNA‑binding transcriptional regulator CreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,5910CT93.3% 124.8 / 0.4 47V195V (GTC→GTT) creBDNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (27/15);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

AGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCA  >  NC_000913/4636346‑4636833
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGACGTCTCCCGTCATACGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                                                                                                               >  1:197167/1‑251 (MQ=255)
    tGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCTTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCACGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGAGCAGGACACCCACGAACGCACCGTTGATCCCcac                                                                                                                                                                                                                                           >  2:419354/1‑251 (MQ=255)
    tGCGCACCTTACTGCGTAGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGAACGCACCGTTGATACCcac                                                                                                                                                                                                                                           >  2:326222/1‑251 (MQ=255)
              aCTGCGTCGGGTGAAGAAATTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAAcct                                                                                                                                                                                                                                 >  2:167476/1‑249 (MQ=255)
                 gCGTGGGGGGAAGAAGTTCTCTTCGCCGCCCCCCTTCCTCCGTTTTGGTCATTT‑GAATTTGAAGGACCCCGCGGCGCAGACCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                                                                                                               <  1:394652/250‑4 (MQ=255)
                  cGTCGGGTGAAGAAGTTCTCGACGCAGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGAT‑GGATTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTAGCCAGTCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGGTACCGGGGGGGAAGATGCGAAGGACCCCTAAGATAGCACAGTTGATACCCACATTAAAACGTTGCg                                                                                                                                                                                                                             >  2:387618/1‑251 (MQ=255)
                   gtggggtaaaaaagttccggaggcggTCTCCCGTCACCCGTGTGGGAAATTTTAAATG‑AAATGACCCCGGGGCGCAGA‑CAACTTGGTTGGACACGCCATTAGCGCTGACTCGTGT‑TGAGTTTTTATTGGGGAAGCCGTTCCTCAAGTCCCCGGGCCGCGTTGGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGt                                                                                                                                                                                                                            <  2:73296/226‑1 (MQ=255)
                         caccccccttttccgatctgtcccccGTCATCCGTTTTGGTAATTTGAAATT‑AAATAAACCCGGGGCGCATATCAGCT‑GTTTTGCCACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                                                                      <  2:202321/228‑1 (MQ=255)
                                         gCCGTCTCCCGTCCTCCGTATTGGTCATTTTGAATT‑GAATGAACCAGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GAATGAGTTTTTATTGCTGAAGACGTTACTAAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCCGGACACCTACGATCGACCCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCGGCGCGCCATCAAcc                                                                                                                                                                                                      >  2:448636/1‑251 (MQ=255)
                                            gTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                                                                      >  1:202321/1‑232 (MQ=255)
                                                      aTCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCAACATTAAAACGCTGCGTGCCAAGCTGCGCGCCCTCAACCCCGCTCTTTCAcc                                                                                                                                                                                         >  2:450372/1‑251 (MQ=255)
                                                               ggtcATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGCGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATAc                                                                                                                                                                                >  1:288939/4‑251 (MQ=255)
                                                                        gaagt‑gaatgaaCCCGCGGCACAGATCGGCG‑GGTTTGACACGCCATTAACGCTGACGCG‑GTTTGATTTTTTATTGCGGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                                                                                                                                        <  1:342061/246‑1 (MQ=255)
                                                                               aatgaaCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGGCATggg                                                                                                                                                                 >  1:278632/1‑251 (MQ=255)
                                                                                   accccGCGGCGCAGATCAGTT‑GGTTTGACACGCCATTAACGCTGCCGCG‑TTATGATTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                                                             <  1:257047/249‑1 (MQ=255)
                                                                                                 aTCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGTCATGGGGTCTAGCCTGGGGGGCCt                                                                                                                                               >  2:178870/1‑251 (MQ=255)
                                                                                                    aagttggttggccacGCCATTAACGCGGACGGG‑GTATGAGTTTTTATTGCTGAAGACGTTCCTCAAGCCGCGAGGCCGCGTCTGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                                                                                                             <  1:167476/239‑1 (MQ=255)
                                                                                                                               gacgcG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTCTCGGCATGCGGTTgctgct                                                                                                                  >  1:72510/5‑251 (MQ=255)
                                                                                                                                                ttATTGCTAAAGACTTTCCTCAAGTCGCCGGGCCGGGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt                                                                                                   <  1:329550/250‑1 (MQ=255)
                                                                                                                                                  aTTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACtggt                                                                                                >  2:465967/1‑251 (MQ=255)
                                                                                                                                                     gctgAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTAACGGCATGCGGTTCCTGCTGGGCTATTTTTTACtggtggg                                                                                             >  2:103341/3‑250 (MQ=255)
                                                                                                                                                         aaGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACAGTTGCTACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGAAATGCGTATCGGCATGCGGTTGCTGCTGGGCAATTTTTTACTggtggaggtg                                                                                         >  1:321551/1‑251 (MQ=255)
                                                                                                                                                           gACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGAGTTTTTTTACTggtggcggtggg                                                                                       >  2:89134/1‑250 (MQ=255)
                                                                                                                                                              gTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                                                             >  2:164229/1‑178 (MQ=255)
                                                                                                                                                              gTTACTAAAGTCGCCAGGCGGCGTCGGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTGTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                                                             <  1:164229/178‑1 (MQ=255)
                                                                                                                                                                    cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTgg                                                                                <  1:15205/249‑1 (MQ=255)
                                                                                                                                                                                ccGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGctat                                                                  <  1:11497/251‑4 (MQ=255)
                                                                                                                                                                                  gcgTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttt                                                                >  1:303880/1‑251 (MQ=255)
                                                                                                                                                                                    gTCTGGTCCCGCCAGCAA‑TGAATGGATAGCGTGTGGGAAGTTGCGCAGGACGCCTACGATCGCACCGTTGAAACCCACATTAAAACGTTGCGTGGCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATGTTTTACTGGTGGCGGTTGCGGCCTGGTTCGTACTGGCTAttttt                                                              <  1:311463/251‑1 (MQ=255)
                                                                                                                                                                                     tCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGGGGCGGCCTGGTTCGTACTGGctaattttt                                                             >  1:99186/1‑243 (MQ=255)
                                                                                                                                                                                       tGGTCCCGCCAGCAACTAA‑TGGTAAGCGTGTGGGACGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGGGATATAGCCTGAGGGGACTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTAATTGTGGCGGTGgccgccagtcttggacaggccttttttgct                                                           >  2:124615/1‑227 (MQ=255)
                                                                                                                                                                                        ggccccGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGt                                                            <  1:309595/246‑1 (MQ=255)
                                                                                                                                                                                                aaGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCGTGCCATCAACCCTGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGGATCGGCATGCGGTTTCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGTTGCGTACTGGCTATTTTTGTCAAAGAAGtt                                                  <  1:409656/250‑1 (MQ=255)
                                                                                                                                                                                                  gCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTa                                                 >  1:397315/1‑250 (MQ=255)
                                                                                                                                                                                                   cAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGACTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGAAGTTaa                                                >  1:485483/1‑250 (MQ=255)
                                                                                                                                                                                                     aCTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCTGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAcc                                             <  1:450372/251‑1 (MQ=255)
                                                                                                                                                                                                      ctaa‑TGGCTAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGCTCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTTGTTCTGGCTATTTTTGTTAAAGAAGTTAAACCg                                            >  2:456642/4‑251 (MQ=255)
                                                                                                                                                                                                        aa‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCg                                            <  1:456642/248‑1 (MQ=255)
                                                                                                                                                                                                                       gggAAGCTGCGCATGACACCTCCGATCGCACCGTTGATACCCAAATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGCTCTTTCACCGATTACTACTAATCGCGGCATGGGCTATAGCCGGAGGGGCCTGTAATGCGTATCGGCATGCGGGTGCTGCTGGGCTATTTTTTACTGGTGGAGGTGGCGGCCTGGTTCGGACTGGCACTTTTTTTCAaagaaggtcacacggggcggcgacgggccg                            >  2:432334/1‑227 (MQ=255)
                                                                                                                                                                                                                        ggAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAc                           <  1:150218/251‑1 (MQ=255)
                                                                                                                                                                                                                         gAAGATGCGCCGGACACCTACGATCGCTCCGTTGATACCCACATTAAAACGTGGCGGGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACg                          <  2:172694/251‑1 (MQ=255)
                                                                                                                                                                                                                            gATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCACAGAAGTTAAACCGGGCGTGCGAAGAGCAACgg                         >  1:189281/1‑249 (MQ=255)
                                                                                                                                                                                                                                 gcAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGACGTTAAACCGGGCGTGCGAAGAGCCACGgggggg                    >  2:500954/1‑249 (MQ=255)
                                                                                                                                                                                                                                  cAGGACACCTACGAGCGCACCGTGGACACCCACATTAAAACGTTGCGTTCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCTCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTCTTACTGGTGGCGGTGGCGGCCTGGTTCGTATTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACg                 <  1:170/251‑1 (MQ=255)
                                                                                                                                                                                                                                       cacatACGATCGCACCGTTGATACCCACATTAACCCGTTGCGTACCAAGCTGCGCGCCATCAACCCCGATCTTCCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGGACTGGCCATTTTTGTTAACGTTGTTAAAACGGGCGGGCGGAGAGCAACG‑Aggggggagttta            >  2:326001/5‑242 (MQ=255)
                                                                                                                                                                                                                                        acCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCATGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGGGGAGGTGACGGCCTGGTTAGTAATGGCTAATTTTGTCAAAGCAGGTAAATCGGGCGTGCGAATAGCAAAGGTGGGGACGttaat            >  1:300371/1‑247 (MQ=255)
                                                                                                                                                                                                                                                  gCACCGTAGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCACAGAAGGTAAACCGGGGGTGCGAAGGGCAAAGGGGGGGGCGttactagataccacc  >  2:358622/1‑237 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                  
AGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCA  >  NC_000913/4636346‑4636833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑