Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,636,555 A→G V183V (GTA→GTG)  creB → DNA‑binding transcriptional regulator CreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,5550AG92.9% 106.6 / 0.6 42V183V (GTA→GTG) creBDNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (25/14);  total (26/16)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.88e-01

GGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCG  >  NC_000913/4636312‑4636791
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
ggCTAAACCGCTTTCACCCCGCGAAGTGTGCGCAAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGACTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAA‑TGGATAGCATGTGGGa                                                                                                                                                                                                                                         >  1:181492/1‑249 (MQ=255)
          ttttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGACTGGTCCCGCCCGCAACTAA‑TGGATAGCGGGTGGGAAGATgcgccgg                                                                                                                                                                                                                              >  2:413571/1‑247 (MQ=255)
              cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACAcc                                                                                                                                                                                                                         >  1:167717/1‑251 (MQ=255)
                          ggggcgcCAGGGTGCGCACTTTACTGCGTCGGGTGAAGAATTTCTCGTCGCCTTCCCCCGTCATCCGTATTGGTCATTTTGAATT‑AAATGACCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCAcc                                                                                                                                                                                                             <  1:262215/248‑1 (MQ=255)
                                  aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGACGTCTCCCGTCATACGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                                                                     >  1:197167/1‑251 (MQ=255)
                                      tGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCTTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCACGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGAGCAGGACACCCACGAACGCACCGTTGATCCCcac                                                                                                                                                                                                 >  2:419354/1‑251 (MQ=255)
                                      tGCGCACCTTACTGCGTAGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGAACGCACCGTTGATACCcac                                                                                                                                                                                                 >  2:326222/1‑251 (MQ=255)
                                                aCTGCGTCGGGTGAAGAAATTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAAcct                                                                                                                                                                                       >  2:167476/1‑249 (MQ=255)
                                                   gCGTGGGGGGAAGAAGTTCTCTTCGCCGCCCCCCTTCCTCCGTTTTGGTCATTT‑GAATTTGAAGGACCCCGCGGCGCAGACCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                                                                     <  1:394652/250‑4 (MQ=255)
                                                    cGTCGGGTGAAGAAGTTCTCGACGCAGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGAT‑GGATTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTAGCCAGTCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGGTACCGGGGGGGAAGATGCGAAGGACCCCTAAGATAGCACAGTTGATACCCACATTAAAACGTTGCg                                                                                                                                                                                   >  2:387618/1‑251 (MQ=255)
                                                     gtggggtaaaaaagttccggaggcggTCTCCCGTCACCCGTGTGGGAAATTTTAAATG‑AAATGACCCCGGGGCGCAGA‑CAACTTGGTTGGACACGCCATTAGCGCTGACTCGTGT‑TGAGTTTTTATTGGGGAAGCCGTTCCTCAAGTCCCCGGGCCGCGTTGGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGt                                                                                                                                                                                  <  2:73296/226‑1 (MQ=255)
                                                           caccccccttttccgatctgtcccccGTCATCCGTTTTGGTAATTTGAAATT‑AAATAAACCCGGGGCGCATATCAGCT‑GTTTTGCCACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                            <  2:202321/228‑1 (MQ=255)
                                                                           gCCGTCTCCCGTCCTCCGTATTGGTCATTTTGAATT‑GAATGAACCAGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GAATGAGTTTTTATTGCTGAAGACGTTACTAAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCCGGACACCTACGATCGACCCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCGGCGCGCCATCAAcc                                                                                                                                                            >  2:448636/1‑251 (MQ=255)
                                                                              gTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                            >  1:202321/1‑232 (MQ=255)
                                                                                        aTCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCAACATTAAAACGCTGCGTGCCAAGCTGCGCGCCCTCAACCCCGCTCTTTCAcc                                                                                                                                               >  2:450372/1‑251 (MQ=255)
                                                                                                 ggtcATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGCGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATAc                                                                                                                                      >  1:288939/4‑251 (MQ=255)
                                                                                                          gaagt‑gaatgaaCCCGCGGCACAGATCGGCG‑GGTTTGACACGCCATTAACGCTGACGCG‑GTTTGATTTTTTATTGCGGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                                                                                              <  1:342061/246‑1 (MQ=255)
                                                                                                                 aatgaaCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGGCATggg                                                                                                                       >  1:278632/1‑251 (MQ=255)
                                                                                                                     accccGCGGCGCAGATCAGTT‑GGTTTGACACGCCATTAACGCTGCCGCG‑TTATGATTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                   <  1:257047/249‑1 (MQ=255)
                                                                                                                                   aTCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGTCATGGGGTCTAGCCTGGGGGGCCt                                                                                                     >  2:178870/1‑251 (MQ=255)
                                                                                                                                      aagttggttggccacGCCATTAACGCGGACGGG‑GTATGAGTTTTTATTGCTGAAGACGTTCCTCAAGCCGCGAGGCCGCGTCTGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                                                                   <  1:167476/239‑1 (MQ=255)
                                                                                                                                                                 gacgcG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTCTCGGCATGCGGTTgctgct                                                                        >  1:72510/5‑251 (MQ=255)
                                                                                                                                                                                  ttATTGCTAAAGACTTTCCTCAAGTCGCCGGGCCGGGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt                                                         <  1:329550/250‑1 (MQ=255)
                                                                                                                                                                                    aTTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACtggt                                                      >  2:465967/1‑251 (MQ=255)
                                                                                                                                                                                       gctgAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTAACGGCATGCGGTTCCTGCTGGGCTATTTTTTACtggtggg                                                   >  2:103341/3‑250 (MQ=255)
                                                                                                                                                                                           aaGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACAGTTGCTACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGAAATGCGTATCGGCATGCGGTTGCTGCTGGGCAATTTTTTACTggtggaggtg                                               >  1:321551/1‑251 (MQ=255)
                                                                                                                                                                                             gACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGAGTTTTTTTACTggtggcggtggg                                             >  2:89134/1‑250 (MQ=255)
                                                                                                                                                                                                gTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                   >  2:164229/1‑178 (MQ=255)
                                                                                                                                                                                                gTTACTAAAGTCGCCAGGCGGCGTCGGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTGTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                   <  1:164229/178‑1 (MQ=255)
                                                                                                                                                                                                      cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTgg                                      <  1:15205/249‑1 (MQ=255)
                                                                                                                                                                                                                  ccGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGctat                        <  1:11497/251‑4 (MQ=255)
                                                                                                                                                                                                                    gcgTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttt                      >  1:303880/1‑251 (MQ=255)
                                                                                                                                                                                                                      gTCTGGTCCCGCCAGCAA‑TGAATGGATAGCGTGTGGGAAGTTGCGCAGGACGCCTACGATCGCACCGTTGAAACCCACATTAAAACGTTGCGTGGCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATGTTTTACTGGTGGCGGTTGCGGCCTGGTTCGTACTGGCTAttttt                    <  1:311463/251‑1 (MQ=255)
                                                                                                                                                                                                                       tCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGGGGCGGCCTGGTTCGTACTGGctaattttt                   >  1:99186/1‑243 (MQ=255)
                                                                                                                                                                                                                         tGGTCCCGCCAGCAACTAA‑TGGTAAGCGTGTGGGACGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGGGATATAGCCTGAGGGGACTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTAATTGTGGCGGTGgccgccagtcttggacaggccttttttgct                 >  2:124615/1‑227 (MQ=255)
                                                                                                                                                                                                                          ggccccGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGt                  <  1:309595/246‑1 (MQ=255)
                                                                                                                                                                                                                                  aaGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCGTGCCATCAACCCTGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGGATCGGCATGCGGTTTCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGTTGCGTACTGGCTATTTTTGTCAAAGAAGtt        <  1:409656/250‑1 (MQ=255)
                                                                                                                                                                                                                                    gCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTa       >  1:397315/1‑250 (MQ=255)
                                                                                                                                                                                                                                     cAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGACTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGAAGTTaa      >  1:485483/1‑250 (MQ=255)
                                                                                                                                                                                                                                       aCTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCTGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAcc   <  1:450372/251‑1 (MQ=255)
                                                                                                                                                                                                                                        ctaa‑TGGCTAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGCTCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTTGTTCTGGCTATTTTTGTTAAAGAAGTTAAACCg  >  2:456642/4‑251 (MQ=255)
                                                                                                                                                                                                                                          aa‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCg  <  1:456642/248‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                            
GGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCG  >  NC_000913/4636312‑4636791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑