Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,543	G→A	L179L (CTG→CTA)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,543	0	G	A	90.0%	99.0 / 2.2	40	L179L (CTG→CTA)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (24/12);  total (25/15)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACC  >  NC_000913/4636312‑4636790                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                         ggCTAAACCGCTTTCACCCCGCGAAGTGTGCGCAAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGACTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAA‑TGGATAGCATGTGGGa                                                                                                                                                                                                                                        >  1:181492/1‑249 (MQ=255)           ttttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGACTGGTCCCGCCCGCAACTAA‑TGGATAGCGGGTGGGAAGATgcgccgg                                                                                                                                                                                                                             >  2:413571/1‑247 (MQ=255)               cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACAcc                                                                                                                                                                                                                        >  1:167717/1‑251 (MQ=255)                           ggggcgcCAGGGTGCGCACTTTACTGCGTCGGGTGAAGAATTTCTCGTCGCCTTCCCCCGTCATCCGTATTGGTCATTTTGAATT‑AAATGACCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCAcc                                                                                                                                                                                                            <  1:262215/248‑1 (MQ=255)                                   aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGACGTCTCCCGTCATACGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                                                                    >  1:197167/1‑251 (MQ=255)                                       tGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCTTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCACGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGAGCAGGACACCCACGAACGCACCGTTGATCCCcac                                                                                                                                                                                                >  2:419354/1‑251 (MQ=255)                                       tGCGCACCTTACTGCGTAGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGAACGCACCGTTGATACCcac                                                                                                                                                                                                >  2:326222/1‑251 (MQ=255)                                                 aCTGCGTCGGGTGAAGAAATTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAAcct                                                                                                                                                                                      >  2:167476/1‑249 (MQ=255)                                                    gCGTGGGGGGAAGAAGTTCTCTTCGCCGCCCCCCTTCCTCCGTTTTGGTCATTT‑GAATTTGAAGGACCCCGCGGCGCAGACCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                                                                    <  1:394652/250‑4 (MQ=255)                                                     cGTCGGGTGAAGAAGTTCTCGACGCAGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGAT‑GGATTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTAGCCAGTCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGGTACCGGGGGGGAAGATGCGAAGGACCCCTAAGATAGCACAGTTGATACCCACATTAAAACGTTGCg                                                                                                                                                                                  >  2:387618/1‑251 (MQ=255)                                                      gtggggtaaaaaagttccggaggcggTCTCCCGTCACCCGTGTGGGAAATTTTAAATG‑AAATGACCCCGGGGCGCAGA‑CAACTTGGTTGGACACGCCATTAGCGCTGACTCGTGT‑TGAGTTTTTATTGGGGAAGCCGTTCCTCAAGTCCCCGGGCCGCGTTGGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGt                                                                                                                                                                                 <  2:73296/226‑1 (MQ=255)                                                            caccccccttttccgatctgtcccccGTCATCCGTTTTGGTAATTTGAAATT‑AAATAAACCCGGGGCGCATATCAGCT‑GTTTTGCCACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                           <  2:202321/228‑1 (MQ=255)                                                                            gCCGTCTCCCGTCCTCCGTATTGGTCATTTTGAATT‑GAATGAACCAGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GAATGAGTTTTTATTGCTGAAGACGTTACTAAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCCGGACACCTACGATCGACCCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCGGCGCGCCATCAAcc                                                                                                                                                           >  2:448636/1‑251 (MQ=255)                                                                               gTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                                                           >  1:202321/1‑232 (MQ=255)                                                                                         aTCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCAACATTAAAACGCTGCGTGCCAAGCTGCGCGCCCTCAACCCCGCTCTTTCAcc                                                                                                                                              >  2:450372/1‑251 (MQ=255)                                                                                                  ggtcATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGCGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATAc                                                                                                                                     >  1:288939/4‑251 (MQ=255)                                                                                                           gaagt‑gaatgaaCCCGCGGCACAGATCGGCG‑GGTTTGACACGCCATTAACGCTGACGCG‑GTTTGATTTTTTATTGCGGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                                                                                             <  1:342061/246‑1 (MQ=255)                                                                                                                  aatgaaCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGGCATggg                                                                                                                      >  1:278632/1‑251 (MQ=255)                                                                                                                      accccGCGGCGCAGATCAGTT‑GGTTTGACACGCCATTAACGCTGCCGCG‑TTATGATTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                  <  1:257047/249‑1 (MQ=255)                                                                                                                                    aTCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGTCATGGGGTCTAGCCTGGGGGGCCt                                                                                                    >  2:178870/1‑251 (MQ=255)                                                                                                                                       aagttggttggccacGCCATTAACGCGGACGGG‑GTATGAGTTTTTATTGCTGAAGACGTTCCTCAAGCCGCGAGGCCGCGTCTGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                                                                  <  1:167476/239‑1 (MQ=255)                                                                                                                                                                  gacgcG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTCTCGGCATGCGGTTgctgct                                                                       >  1:72510/5‑251 (MQ=255)                                                                                                                                                                                   ttATTGCTAAAGACTTTCCTCAAGTCGCCGGGCCGGGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt                                                        <  1:329550/250‑1 (MQ=255)                                                                                                                                                                                     aTTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACtggt                                                     >  2:465967/1‑251 (MQ=255)                                                                                                                                                                                        gctgAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTAACGGCATGCGGTTCCTGCTGGGCTATTTTTTACtggtggg                                                  >  2:103341/3‑250 (MQ=255)                                                                                                                                                                                            aaGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACAGTTGCTACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGAAATGCGTATCGGCATGCGGTTGCTGCTGGGCAATTTTTTACTggtggaggtg                                              >  1:321551/1‑251 (MQ=255)                                                                                                                                                                                              gACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGAGTTTTTTTACTggtggcggtggg                                            >  2:89134/1‑250 (MQ=255)                                                                                                                                                                                                 gTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                  >  2:164229/1‑178 (MQ=255)                                                                                                                                                                                                 gTTACTAAAGTCGCCAGGCGGCGTCGGGCCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTGTCACCGATTAATACTCATCGCGGCATGGGatat                                                                                                                  <  1:164229/178‑1 (MQ=255)                                                                                                                                                                                                       cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTgg                                     <  1:15205/249‑1 (MQ=255)                                                                                                                                                                                                                   ccGCGTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGctat                       <  1:11497/251‑4 (MQ=255)                                                                                                                                                                                                                     gcgTCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTAttt                     >  1:303880/1‑251 (MQ=255)                                                                                                                                                                                                                       gTCTGGTCCCGCCAGCAA‑TGAATGGATAGCGTGTGGGAAGTTGCGCAGGACGCCTACGATCGCACCGTTGAAACCCACATTAAAACGTTGCGTGGCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATGTTTTACTGGTGGCGGTTGCGGCCTGGTTCGTACTGGCTAttttt                   <  1:311463/251‑1 (MQ=255)                                                                                                                                                                                                                        tCTGGTCCCGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGGGGCGGCCTGGTTCGTACTGGctaattttt                  >  1:99186/1‑243 (MQ=255)                                                                                                                                                                                                                          tGGTCCCGCCAGCAACTAA‑TGGTAAGCGTGTGGGACGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCCTGGGATATAGCCTGAGGGGACTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTAATTGTGGCGGTGgccgccagtcttggacaggccttttttgct                >  2:124615/1‑227 (MQ=255)                                                                                                                                                                                                                           ggccccGCCAGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGt                 <  1:309595/246‑1 (MQ=255)                                                                                                                                                                                                                                   aaGCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAACCGTTGCGTGCCAAGCTGCGTGCCATCAACCCTGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGGATCGGCATGCGGTTTCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGTTGCGTACTGGCTATTTTTGTCAAAGAAGtt       <  1:409656/250‑1 (MQ=255)                                                                                                                                                                                                                                     gCAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTa      >  1:397315/1‑250 (MQ=255)                                                                                                                                                                                                                                      cAACTAA‑TGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGACTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGGTCGTACTGGCTATTTTTGTCAAAGAAGTTaa     >  1:485483/1‑250 (MQ=255)                                                                                                                                                                                                                                        aCTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCTGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAAcc  <  1:450372/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                         GGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGA‑TGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACC  >  NC_000913/4636312‑4636790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑