Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,513	A→G	S169S (TCA→TCG)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,513	0	A	G	97.1%	96.2 / ‑4.3	36	S169S (TCA→TCG)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (21/13);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

CGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGG  >  NC_000913/4636274‑4636755                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   cGCCTGCTTGGGCTGGAAATTGGTGCTGCCGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCCTTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTAATCAAGTCGCCAGGCCGCGt                                                                                                                                                                                                                                         >  2:492715/1‑251 (MQ=255)   ccTGCTTGGGCTGGAAATTGGTGCTGACGTCTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCt                                                                                                                                                                                                                                       <  1:346616/251‑1 (MQ=255)          gggCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGGCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTccc                                                                                                                                                                                                                                 <  1:492715/250‑1 (MQ=255)           ggCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCCCCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGc                                                                                                                                                                                                                               <  1:374008/251‑1 (MQ=255)               ggAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCcgcg                                                                                                                                                                                                                                          >  1:138445/1‑236 (MQ=255)               ggAAATTGGGGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCcgcg                                                                                                                                                                                                                                          <  2:138445/236‑1 (MQ=255)                                       ggCTAAACCGCTTTCACCCCGCGAAGTGTGCGCAAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGACTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCATGTGGGa                                                                                                                                                                                                     >  1:181492/1‑249 (MQ=255)                                                 ttttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGACTGGTCCCGCCCGCAACTAATGGATAGCGGGTGGGAAGATgcgccgg                                                                                                                                                                                          >  2:413571/1‑247 (MQ=255)                                                     cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACAcc                                                                                                                                                                                     >  1:167717/1‑251 (MQ=255)                                                                 ggggcgcCAGGGTGCGCACTTTACTGCGTCGGGTGAAGAATTTCTCGTCGCCTTCCCCCGTCATCCGTATTGGTCATTTTGAATT‑AAATGACCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCAcc                                                                                                                                                                         <  1:262215/248‑1 (MQ=255)                                                                         aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGACGTCTCCCGTCATACGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                                 >  1:197167/1‑251 (MQ=255)                                                                             tGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCTTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCACGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGAGCAGGACACCCACGAACGCACCGTTGATCCCcac                                                                                                                                                             >  2:419354/1‑251 (MQ=255)                                                                             tGCGCACCTTACTGCGTAGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGAACGCACCGTTGATACCcac                                                                                                                                                             >  2:326222/1‑251 (MQ=255)                                                                                       aCTGCGTCGGGTGAAGAAATTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAAcct                                                                                                                                                   >  2:167476/1‑249 (MQ=255)                                                                                          gCGTGGGGGGAAGAAGTTCTCTTCGCCGCCCCCCTTCCTCCGTTTTGGTCATTT‑GAATTTGAAGGACCCCGCGGCGCAGACCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                                 <  1:394652/250‑4 (MQ=255)                                                                                           cGTCGGGTGAAGAAGTTCTCGACGCAGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGAT‑GGATTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTAGCCAGTCCGCGTCTGGTCCCGCCAGCAACTAATGGGTACCGGGGGGGAAGATGCGAAGGACCCCTAAGATAGCACAGTTGATACCCACATTAAAACGTTGCg                                                                                                                                               >  2:387618/1‑251 (MQ=255)                                                                                            gtggggtaaaaaagttccggaggcggTCTCCCGTCACCCGTGTGGGAAATTTTAAATG‑AAATGACCCCGGGGCGCAGA‑CAACTTGGTTGGACACGCCATTAGCGCTGACTCGTGT‑TGAGTTTTTATTGGGGAAGCCGTTCCTCAAGTCCCCGGGCCGCGTTGGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGt                                                                                                                                              <  2:73296/226‑1 (MQ=255)                                                                                                  caccccccttttccgatctgtcccccGTCATCCGTTTTGGTAATTTGAAATT‑AAATAAACCCGGGGCGCATATCAGCT‑GTTTTGCCACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                        <  2:202321/228‑1 (MQ=255)                                                                                                                  gCCGTCTCCCGTCCTCCGTATTGGTCATTTTGAATT‑GAATGAACCAGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GAATGAGTTTTTATTGCTGAAGACGTTACTAAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCCGGACACCTACGATCGACCCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCGGCGCGCCATCAAcc                                                                                                                        >  2:448636/1‑251 (MQ=255)                                                                                                                     gTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                                        >  1:202321/1‑232 (MQ=255)                                                                                                                               aTCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCAACATTAAAACGCTGCGTGCCAAGCTGCGCGCCCTCAACCCCGCTCTTTCAcc                                                                                                           >  2:450372/1‑251 (MQ=255)                                                                                                                                        ggtcATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGCGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATAc                                                                                                  >  1:288939/4‑251 (MQ=255)                                                                                                                                                 gaagt‑gaatgaaCCCGCGGCACAGATCGGCG‑GGTTTGACACGCCATTAACGCTGACGCG‑GTTTGATTTTTTATTGCGGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                                                          <  1:342061/246‑1 (MQ=255)                                                                                                                                                        aatgaaCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGGCATggg                                                                                   >  1:278632/1‑251 (MQ=255)                                                                                                                                                            accccGCGGCGCAGATCAGTT‑GGTTTGACACGCCATTAACGCTGCCGCG‑TTATGATTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                               <  1:257047/249‑1 (MQ=255)                                                                                                                                                                          aTCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGTCATGGGGTCTAGCCTGGGGGGCCt                                                                 >  2:178870/1‑251 (MQ=255)                                                                                                                                                                             aagttggttggccacGCCATTAACGCGGACGGG‑GTATGAGTTTTTATTGCTGAAGACGTTCCTCAAGCCGCGAGGCCGCGTCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                                               <  1:167476/239‑1 (MQ=255)                                                                                                                                                                                                        gacgcG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTCTCGGCATGCGGTTgctgct                                    >  1:72510/5‑251 (MQ=255)                                                                                                                                                                                                                         ttATTGCTAAAGACTTTCCTCAAGTCGCCGGGCCGGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt                     <  1:329550/250‑1 (MQ=255)                                                                                                                                                                                                                           aTTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACtggt                  >  2:465967/1‑251 (MQ=255)                                                                                                                                                                                                                              gctgAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTAACGGCATGCGGTTCCTGCTGGGCTATTTTTTACtggtggg               >  2:103341/3‑250 (MQ=255)                                                                                                                                                                                                                                  aaGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACAGTTGCTACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGAAATGCGTATCGGCATGCGGTTGCTGCTGGGCAATTTTTTACTggtggaggtg           >  1:321551/1‑251 (MQ=255)                                                                                                                                                                                                                                    gACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGAGTTTTTTTACTggtggcggtggg         >  2:89134/1‑250 (MQ=255)                                                                                                                                                                                                                                       gTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                                               >  2:164229/1‑178 (MQ=255)                                                                                                                                                                                                                                       gTTACTAAAGTCGCCAGGCGGCGTCGGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTGTCACCGATTAATACTCATCGCGGCATGGGatat                                                                               <  1:164229/178‑1 (MQ=255)                                                                                                                                                                                                                                             cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTgg  <  1:15205/249‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   CGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGG  >  NC_000913/4636274‑4636755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑