Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,636,493 T→C L163L (TTG→CTG)  creB → DNA‑binding transcriptional regulator CreB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,636,4930TC97.2% 102.5 / ‑4.1 37L163L (TTG→CTG) creBDNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (20/15);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

GACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGT  >  NC_000913/4636249‑4636739
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
aaCGGCCCGAAGGGACGGGGTCGAGTGCCTGCTTGGGTGGGAAATTGGTGCTGACGACTATGTGGCTAACCCGTTTCCACCCCGCGAAGTGGGCTCGAGGTTGCGCACCTTACGTCGTCGGGTGAAGAAGTTCTAGTAGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGTGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGa                                                                                                                                                                                                                                                  <  2:239618/250‑1 (MQ=255)
     cccGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTa                                                                                                                                                                                                                                             <  1:159992/251‑1 (MQ=255)
      ccGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGCAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTAc                                                                                                                                                                                                                                            >  1:200455/1‑251 (MQ=255)
             gAAGAGGGCGATCGCTTGCTGGGGCAGTAAATTGGTGCTGACGACTATGTGGCTAAACCTTTTTCACCCCGCGAAGTGTGCTCCAGGGTGCGTATCTTACGGCGTCGGGTAAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTTCTGAAGACGGTACTCAAGTc                                                                                                                                                                                                                                     <  1:436268/251‑1 (MQ=255)
              aaGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCCCCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTcg                                                                                                                                                                                                                                    <  1:224148/251‑2 (MQ=255)
                  ggTCGATCGCCTGCTTGGGCTGGAAATTGGTGATGAAGACTATGTGGATAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGtggcc                                                                                                                                                                                                                                  >  1:435995/1‑245 (MQ=255)
                    tCGATCGCCTGCTTGGGCTGGAAATTGGGGCTGACGACTATGTGGCGACCCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGACCGAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTAGTCGTTTTGAATT‑GAATGAACCCTCGGCGCAGATACGCT‑GGTTTGACACGCCATTATAGCTGCCGCG‑GTATGAGTTTTTATTGCTGAAGCCGTTACTCacatcgccggcc                                                                                                                                                                                                                              >  2:170/1‑240 (MQ=255)
                         cGCCTGCTTGGGCTGGAAATTGGTGCTGCCGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCCTTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTAATCAAGTCGCCAGGCCGCGt                                                                                                                                                                                                                         >  2:492715/1‑251 (MQ=255)
                           ccTGCTTGGGCTGGAAATTGGTGCTGACGTCTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCt                                                                                                                                                                                                                       <  1:346616/251‑1 (MQ=255)
                                  gggCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGGCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTccc                                                                                                                                                                                                                 <  1:492715/250‑1 (MQ=255)
                                   ggCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCCCCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGc                                                                                                                                                                                                               <  1:374008/251‑1 (MQ=255)
                                       ggAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCcgcg                                                                                                                                                                                                                          >  1:138445/1‑236 (MQ=255)
                                       ggAAATTGGGGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCcgcg                                                                                                                                                                                                                          <  2:138445/236‑1 (MQ=255)
                                                               ggCTAAACCGCTTTCACCCCGCGAAGTGTGCGCAAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGACTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCATGTGGGa                                                                                                                                                                                     >  1:181492/1‑249 (MQ=255)
                                                                         ttttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGACTGGTCCCGCCCGCAACTAATGGATAGCGGGTGGGAAGATgcgccgg                                                                                                                                                                          >  2:413571/1‑247 (MQ=255)
                                                                             cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACAcc                                                                                                                                                                     >  1:167717/1‑251 (MQ=255)
                                                                                         ggggcgcCAGGGTGCGCACTTTACTGCGTCGGGTGAAGAATTTCTCGTCGCCTTCCCCCGTCATCCGTATTGGTCATTTTGAATT‑AAATGACCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCAcc                                                                                                                                                         <  1:262215/248‑1 (MQ=255)
                                                                                                 aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGACGTCTCCCGTCATACGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                 >  1:197167/1‑251 (MQ=255)
                                                                                                     tGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCTTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCACGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGAGCAGGACACCCACGAACGCACCGTTGATCCCcac                                                                                                                                             >  2:419354/1‑251 (MQ=255)
                                                                                                     tGCGCACCTTACTGCGTAGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGAACGCACCGTTGATACCcac                                                                                                                                             >  2:326222/1‑251 (MQ=255)
                                                                                                               aCTGCGTCGGGTGAAGAAATTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAAcct                                                                                                                                   >  2:167476/1‑249 (MQ=255)
                                                                                                                  gCGTGGGGGGAAGAAGTTCTCTTCGCCGCCCCCCTTCCTCCGTTTTGGTCATTT‑GAATTTGAAGGACCCCGCGGCGCAGACCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACgttg                                                                                                                                 <  1:394652/250‑4 (MQ=255)
                                                                                                                   cGTCGGGTGAAGAAGTTCTCGACGCAGTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGAT‑GGATTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTAGCCAGTCCGCGTCTGGTCCCGCCAGCAACTAATGGGTACCGGGGGGGAAGATGCGAAGGACCCCTAAGATAGCACAGTTGATACCCACATTAAAACGTTGCg                                                                                                                               >  2:387618/1‑251 (MQ=255)
                                                                                                                    gtggggtaaaaaagttccggaggcggTCTCCCGTCACCCGTGTGGGAAATTTTAAATG‑AAATGACCCCGGGGCGCAGA‑CAACTTGGTTGGACACGCCATTAGCGCTGACTCGTGT‑TGAGTTTTTATTGGGGAAGCCGTTCCTCAAGTCCCCGGGCCGCGTTGGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGt                                                                                                                              <  2:73296/226‑1 (MQ=255)
                                                                                                                          caccccccttttccgatctgtcccccGTCATCCGTTTTGGTAATTTGAAATT‑AAATAAACCCGGGGCGCATATCAGCT‑GTTTTGCCACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                        <  2:202321/228‑1 (MQ=255)
                                                                                                                                          gCCGTCTCCCGTCCTCCGTATTGGTCATTTTGAATT‑GAATGAACCAGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GAATGAGTTTTTATTGCTGAAGACGTTACTAAAGTCGCCAGGCCGCGTATGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCCGGACACCTACGATCGACCCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCGGCGCGCCATCAAcc                                                                                                        >  2:448636/1‑251 (MQ=255)
                                                                                                                                             gTCTCCCGTCATCCGTATTGGTCATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAg                                                                                                                        >  1:202321/1‑232 (MQ=255)
                                                                                                                                                       aTCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCAACATTAAAACGCTGCGTGCCAAGCTGCGCGCCCTCAACCCCGCTCTTTCAcc                                                                                           >  2:450372/1‑251 (MQ=255)
                                                                                                                                                                ggtcATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGCGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATAc                                                                                  >  1:288939/4‑251 (MQ=255)
                                                                                                                                                                         gaagt‑gaatgaaCCCGCGGCACAGATCGGCG‑GGTTTGACACGCCATTAACGCTGACGCG‑GTTTGATTTTTTATTGCGGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATcgcg                                                                          <  1:342061/246‑1 (MQ=255)
                                                                                                                                                                                aatgaaCCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTAACCGATTAATACTCATCGCGGCATggg                                                                   >  1:278632/1‑251 (MQ=255)
                                                                                                                                                                                    accccGCGGCGCAGATCAGTT‑GGTTTGACACGCCATTAACGCTGCCGCG‑TTATGATTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGatat                                                               <  1:257047/249‑1 (MQ=255)
                                                                                                                                                                                                  aTCAGCT‑GGTTTGACACGCCATTAACGCTGACGCG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGTCATGGGGTCTAGCCTGGGGGGCCt                                                 >  2:178870/1‑251 (MQ=255)
                                                                                                                                                                                                     aagttggttggccacGCCATTAACGCGGACGGG‑GTATGAGTTTTTATTGCTGAAGACGTTCCTCAAGCCGCGAGGCCGCGTCTGGCCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGt                                               <  1:167476/239‑1 (MQ=255)
                                                                                                                                                                                                                                gacgcG‑GTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCACGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTCTCGGCATGCGGTTgctgct                    >  1:72510/5‑251 (MQ=255)
                                                                                                                                                                                                                                                 ttATTGCTAAAGACTTTCCTCAAGTCGCCGGGCCGGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt     <  1:329550/250‑1 (MQ=255)
                                                                                                                                                                                                                                                   aTTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACtggt  >  2:465967/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
GACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATT‑GAATGAACCCGCGGCGCAGATCAGCT‑GGTTTGACACGCCATTAGCGCTGACTCG‑GTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGT  >  NC_000913/4636249‑4636739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑