Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,631,245 C→T S171S (AGC→AGT slt → soluble lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,631,2450CT91.5% 147.6 / 5.1 62S171S (AGC→AGTsltsoluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (33/21);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.04e-01

TAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGT  >  NC_000913/4631005‑4631487
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   
tAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGgcgtg                                                                                                                                                                                                                                           >  1:58784/1‑251 (MQ=255)
   ccccACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGCACAGCCAGGAACTCCCGCAACGCAATGTAAATACTACTATGCAAAATGGAAAACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCTAACGCCTGTGACAAGTTATTTAGGGTCTGgcgtgcgt                                                                                                                                                                                                                                        >  1:326873/1‑251 (MQ=255)
        cGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGt                                                                                                                                                                                                                                   <  1:462864/251‑1 (MQ=255)
         gCTTCCTCCCGCTCGCACGCTGCACTCTCGTTTCGTCAATGAACTGGCGCGGCGTGACGACTGGCGTGGCTTGTTTGCCTTTAGCCCGGGAAAGCCCCGAACTACCGCAGCACCATGTAATTACTACTATGCAAAAAGGGAAACCGGGCAGAGTGAAGAAGCCCGGAATGGGGCGAAAGAGCTGTGGCAAACCAGCAAATGCCCACCTAACGCCTGTGTCAAGTGATTTAGAGACTGgcgtgcggccggg                                                                                                                                                                                                                                   >  2:192812/1‑244 (MQ=255)
            tcctccCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCCAAAAGGAACACCGGGCCGAGTGAAGAAGACTGGCACGGGGAGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCCAACGCCTGTGACAAGTTATTTAGTGTCTGgcgggggtaaggaaaac                                                                                                                                                                                                                               >  2:457390/1‑237 (MQ=255)
            tcctccCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTaaa                                                                                                                                                                                                                                <  1:166143/250‑1 (MQ=255)
             cctccCGCACGCACGCTGCAATCTCGTTTCGTCAAGGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACa                                                                                                                                                                                                                              <  1:229383/251‑1 (MQ=255)
              ctccCGCTCGCACGCTGCAATATCGTTTCGTCAATGAACTGGCGCGGCGTGAAGTCTGGCGAGGCTTGTTAGCCTTTAGCCCGGCAAAGGCCGGGACTACCGAAGCGCCATGTAATTTCTACTATGTAAAATGGAACCCCGGGCAGGGTGAAGAAACCTGGCAAGGGGCGCAAGGGCTTGGGCTAACCGGCCAGAGCCCGCCAAAAGCCCTTGAATAGTTATTTAGTGTCTgggcggccgccagtcacca                                                                                                                                                                                                                              >  2:230921/1‑233 (MQ=255)
                    cTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCg                                                                                                                                                                                                                       >  1:303836/1‑251 (MQ=255)
                        cACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTCGCCTTTAGCCCGGAAAAGCCCGGCACAACCCGAGCGCAATGTAATTAATACTCTGCAACATGGCACCCCGGGCAGAGGGAAGAAGCCTGGCAAGGCGCGAAAGAACTGTGGGTACCCCGGAAGAGCCAGCCTCACGACTGTGACACGTTATTTAATGTCTTGGGTGCGTCAGGTCAACAAGTTcccgtgg                                                                                                                                                                                                                   >  1:34970/1‑246 (MQ=255)
                            cTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCCTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGGGAAGAAGCCTGGCAAGGGGCGAAGAAACTGTGGCTAACCGGCAAGAGCCAGCCTACCGACTGGGACAAGTTTTTTAGGGGCTGGCGGGCGGCAGGGAACAAAGATCCAGTGGCGtg                                                                                                                                                                                                               >  2:346872/1‑250 (MQ=255)
                               cAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAACCTTTAGCCCGGAAAAGCCCGGAACTACCCAAGCGCAATGTAATTACTACTATGCAAAATTGGACACCGGGCAGAGGGAAATAGCCTGGCAAGGAGCGAAAGAGATGTTGGTAACAGGCAAGAGCCAGCCCAAAGACTGTGAAAAGTTATTTAGTGTATGGGGTGCGGCAGGTAAACAAGATCAGCTGGCGtcatt                                                                                                                                                                                                            >  1:363077/1‑247 (MQ=255)
                                aaTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGGGCAATGGAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGGCTGGCCAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCTAACGCCTTGGACAAGTTATTTAGTGTCTGGCTGGCGTCAGGGAAACAAGGTCCGCTGGccgtttt                                                                                                                                                                                                            >  2:359457/1‑244 (MQ=255)
                                     cGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGc                                                                                                                                                                                                       <  1:322175/250‑1 (MQ=255)
                                     cGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGGGCGTCAGGTAAACAAGATCCGCTGGCGTATTTaga                                                                                                                                                                                                         <  2:462772/248‑1 (MQ=255)
                                             cAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCGGGCCAGGGGCGAAAGAGCTGTGGCTAACCAGCCAGAGCCAGCCTAAAGCCTGGGACAAGTTATTTAGTGTCTGGCGGGCGGCCGGGAAACAAGATCCGCGGGCGTATTTTGAGCGGATCCggc                                                                                                                                                                                              >  2:362586/1‑249 (MQ=255)
                                              aaTGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTAGGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCt                                                                                                                                                                                             <  2:211951/251‑1 (MQ=255)
                                                       gcgcGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGaa                                                                                                                                                                                    <  1:270817/251‑1 (MQ=255)
                                                        cgcgGCGTGAAGCCTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGGGAAGAAGCCTGGAAAGGGGCGGAAGAGCTGTGGCTAACCGGCAAGAGCCAGGCTAACCCCGGGGGCAAGGTATTTAATGTTCGGCGTGGGTCCGGTAAACAAGGTACGCTGGCGTCTTTTGATCGTAGCCgcgcagcgattaa                                                                                                                                                                                    >  2:85779/1‑238 (MQ=255)
                                                                        gCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACGATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGcc                                                                                                                                                                   <  2:155982/251‑1 (MQ=255)
                                                                          gTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTg                                                                                                                                                                 <  1:360772/251‑1 (MQ=255)
                                                                            ggCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAATCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGt                                                                                                                                                               <  1:109584/251‑1 (MQ=255)
                                                                              cTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGCGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTa                                                                                                                                                              >  1:310795/1‑250 (MQ=255)
                                                                                 gTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACa                                                                                                                                                           <  2:235764/250‑1 (MQ=255)
                                                                                  ttAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGt                                                                                                                                                         >  1:361080/1‑251 (MQ=255)
                                                                                         ttAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggc                                                                                                                                                   <  2:361080/250‑1 (MQ=255)
                                                                                           aGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggcagg                                                                                                                                                >  1:154005/1‑251 (MQ=255)
                                                                                               cGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggcagggc                                                                                                                                              >  1:404282/1‑249 (MQ=255)
                                                                                                 gAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAt                                                                                                                                          <  1:177446/251‑1 (MQ=255)
                                                                                                       ccctgAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCGGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGAtgcctgc                                                                                                                                    <  2:34337/247‑1 (MQ=255)
                                                                                                              cTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAc                                                                                                                            >  1:319224/1‑251 (MQ=255)
                                                                                                                 ccGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAGGCCTGCCGATTAca                                                                                                                           >  2:458308/1‑248 (MQ=255)
                                                                                                                      gcgcAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTAt                                                                                                                    <  1:40670/251‑1 (MQ=255)
                                                                                                                           aTGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGCTTACCAGACTATCGCCt                                                                                                               >  1:31547/1‑251 (MQ=255)
                                                                                                                                 ttACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCa                                                                                                          >  1:50377/1‑250 (MQ=255)
                                                                                                                                   actactAGGCAAAATGGAACCCCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAAt                                                                                                        <  2:58784/250‑1 (MQ=255)
                                                                                                                                            cGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTg                                                                                              >  1:62487/1‑251 (MQ=255)
                                                                                                                                                aTGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCCGACTATCTCCTCGGCAATCATTTCACTGGCg                                                                                           >  1:144105/1‑250 (MQ=255)
                                                                                                                                                     acacCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAacac                                                                                                                                                                        >  2:22046/1‑169 (MQ=255)
                                                                                                                                                     acacCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAacac                                                                                                                                                                        <  1:22046/169‑1 (MQ=255)
                                                                                                                                                          gggCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTa                                                                                 >  1:388873/1‑250 (MQ=255)
                                                                                                                                                             cAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATAc                                                                             >  2:444798/1‑251 (MQ=255)
                                                                                                                                                                agTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACCACCCTAATACggg                                                                          >  1:349708/1‑250 (MQ=255)
                                                                                                                                                                                aaGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGGGCCAAGTTATTTAGTGTCTGGCGTGCGTCCGGTAACCAAGATCAGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCATGGGAACAGTGCTGGCAGGGCAGACGCCTTCCGATTAACCAACTATCGCATCGGCAAACATTCCCCTGGCGAACAACCCTAATaaggacctgccctcagccggt                                                          >  2:503309/1‑231 (MQ=255)
                                                                                                                                                                                 aGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGt                                                          >  1:321845/1‑250 (MQ=255)
                                                                                                                                                                                    ggCGAACGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGACAACAACCCTAATACGGTACTGACCTTCGCCCGTACa                                                       >  2:174226/1‑250 (MQ=255)
                                                                                                                                                                                      cGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACt                                                    <  1:104878/251‑1 (MQ=255)
                                                                                                                                                                                       gAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTg                                                   <  2:62487/251‑1 (MQ=255)
                                                                                                                                                                                        aaaGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTgg                                                  >  1:267145/1‑251 (MQ=255)
                                                                                                                                                                                                 tgGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACCAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAGGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCCCGCGTACAACAGGCGCGACCgg                                         >  2:25789/1‑250 (MQ=255)
                                                                                                                                                                                                   gCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGAtt                                       >  1:15237/1‑251 (MQ=255)
                                                                                                                                                                                                      aaCCGGCAAGAGCCCTCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGTTAAACAAGATCCGCTGGCTTATTTAGATCGTATCCGTCTGTCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGTCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCTCGACCGATTTTa                                    <  1:91667/251‑1 (MQ=255)
                                                                                                                                                                                                         cGGCAAGGGACAGCCTAACGCCTGTGACAAGTTATTTAGTGTCGGGCGTGCGGCAGGGAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACCCTGGCCTTGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTAccc                                 <  2:322880/251‑1 (MQ=255)
                                                                                                                                                                                                            cAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTc                              <  1:458308/251‑1 (MQ=255)
                                                                                                                                                                                                                      ccTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATggcggg                    >  1:22234/1‑250 (MQ=255)
                                                                                                                                                                                                                         aaCGCCGGTGACAAGTTATTTAGTGTCTGGCGTGCGTCATGTAAACAAGATCCGCTGGCGTATGTAGAGCGTATCCGTCTGGCGAGGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAAGCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGgcggcg                   <  2:267145/249‑1 (MQ=255)
                                                                                                                                                                                                                            gCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACACGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGcggcgggggg              >  2:495382/1‑247 (MQ=255)
                                                                                                                                                                                                                               tgtgACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGCGGCGtt           >  1:334620/1‑251 (MQ=255)
                                                                                                                                                                                                                                  gACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCCGATGCCTGCCGATTAACAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATAAGGGACTGACCTTCGCGCGTACAACTGGCGAGACCGATTTTACCCGTCAAAAGGCGGGGGTGGCGttttc        >  2:386628/1‑249 (MQ=255)
                                                                                                                                                                                                                                        ttATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTgggggtttgcccggg  >  2:500479/1‑238 (MQ=255)
                                                                                                                                                                                                                                           tttAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGAtgcc                                                                                                                                       <  1:169280/116‑1 (MQ=255)
                                                                                                                                                                                                                                           tttAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGAtgcc                                                                                                                                       >  2:169280/1‑116 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   
TAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTGGCGAACAACCCTAATACGGTACTGACCTTCGCGCGTACAACTGGCGCGACCGATTTTACCCGTCAAATGGCGGCGGTGGCGTTTGCCAGTGT  >  NC_000913/4631005‑4631487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: