| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,631,146 | G→A | A138A (GCG→GCA) | slt → | soluble lytic murein transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,631,146 | 0 | G | A | 94.9% | 156.9 / ‑0.2 | 61 | A138A (GCG→GCA) | slt | soluble lytic murein transglycosylase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (27/29); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01 | |||||||||||
TGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTG > NC_000913/4630899‑4631395 | ggCCTGGACTGAAAGATTAGCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCTCTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACGTGCGCGGCGTGAATACTGGCGTGGCTTGTTAGTCTTGAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCaaaa < 1:62715/250‑1 (MQ=255) ccTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑ACCCGGAACTACCGGAGCGCCAAT‑TAATTACTACTATGCAAAATg > 1:63547/1‑251 (MQ=255) cTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGAGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGGCTTTAGCCCGGAAAA‑GGCCAGCACTACCGGAGCGC‑ACTGTAATTACTACTATGCCAAATgg > 2:40670/1‑251 (MQ=255) gACTGAAAGATTATCTACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTACCCCCACGCTTCCGCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGTCTGGCGTGGCTTGTTAGCCTTTAGCTCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAac < 2:455632/251‑1 (MQ=255) gACTGAAAGATTATCCCCTTTATCCCTACCTGGAATTCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTGCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAAGGGAac < 2:455641/251‑1 (MQ=255) gACTGAAAGATTATCCACTTTATCCCTACCTTGAATACCGTCAGATCCCCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACCTTGTTCGCGCTAACCCCACGCTTCCGCTCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAac < 1:364007/251‑1 (MQ=255) gACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTTGCCCGGAAAAACCCCGGAA‑TACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAac > 2:177446/1‑251 (MQ=255) ggaaagATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACGCTGCCTCCCGCTCGCACGCTGCAATCTCGTTGCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGTCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACAcc < 2:471795/246‑1 (MQ=255) aTTATCCACTTTATCCCTACCTGGCATACAGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGACCTGGCGCGGCGAGAAGACTGGCGTGGCTTGTTAGACTTTAGACCGGAAAA‑GCCCGGGACTACCGAAGCGG‑AAAGTAATTAATACTATGCAAAATGGAAAACCgggg > 1:357877/1‑248 (MQ=255) ttATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTgaagaa < 1:262683/250‑1 (MQ=255) tATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGaagaag < 1:21120/250‑1 (MQ=255) cccctccctGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACGACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCt < 2:452649/250‑1 (MQ=255) ccggaaacacttcAGATCACCTATGACCTGAGGAACCAACCGGCGGTGCCTGTCACTAACTTTGTTCGCGCTAACCCACCGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATTAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAgg < 2:119721/239‑1 (MQ=255) aGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAGCCCGGGAAA‑ACCCGGAACTACCGAAGCGC‑AATGGAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAAAAGCCTGGCCAGGGGCGAAagaggtg > 1:270689/1‑248 (MQ=255) tgaccTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTAACGAAGCGC‑AAAGTAATTACTACTATGCAAAATGGAACACCGGGACGAGGGAAAAAGCCTGGCACGGGGCGAAAGAGCAGTGGCCAACCg > 1:494586/5‑251 (MQ=255) tgatgaATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACACTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAAATTGCGCGGCGTGAAGACTGGCGTTGCTTGTTAGCCTTTAACCCGGAAAA‑GCCCGGAACTACCCAAGCGC‑AATGTAATTTCAACTTTGTAAAATGGAACACCGGAGAGAGTGAAAAAGCCCTGCAAGGGGGGaaaaacattgggcaaacctgcca > 1:191645/1‑230 (MQ=255) gatgaATCAACCGGCGGTGACTGTCACTCACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGGAAA‑GCCCGGAACTAACGAAGCGC‑AATGTAATTACTAATCAGCACAATGGAACACCGGGCAGAGTGAAGACACCTGGCAAGGGGCGAAAGAGCTGTGGGTAACCgggcaa > 1:196000/1‑246 (MQ=255) atgaATCAACCGGCGGTGACTGTCACTAACGTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAagag < 1:32720/251‑1 (MQ=255) cggcggTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAAc < 1:179769/251‑1 (MQ=255) cggTGACTGTCACTATCTTTGTTCGCTCTAACCCCACGCTGCCTCCCGCTCGCACGCTGCAATCTCTTTGCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACGATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCATGGGGCGAAAGAGCTGTGGCTAACCGGCAAGATCCCGCCTAACGcc < 2:499609/251‑1 (MQ=255) gactgTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCtgtg < 1:261614/247‑1 (MQ=255) gTCACTAACTTTGTTCGCGCTAACCCCCCGCTTCCGCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGAc < 2:396692/249‑1 (MQ=255) tttGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGGGACAAGTTAttt > 1:34337/1‑249 (MQ=255) tGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAgt < 1:118450/250‑2 (MQ=255) gTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTATCCTTTAGCCCGGCAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAAAGCTGTGGCTAACCGGCAAGAGCCAGCCGAACGCCTGGGACAAGTTATTTAgggt > 2:123835/1‑248 (MQ=255) tAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGgcgtg > 1:58784/1‑251 (MQ=255) ccccACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGCACA‑GCCAGGAACTCCCGCAACGC‑AATGTAAATACTACTATGCAAAATGGAAAACCGGGCAGAGTGCAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCTAACGCCTGTGACAAGTTATTTAGGGTCTGgcgtgcgt > 1:326873/1‑251 (MQ=255) cGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGt < 1:462864/251‑1 (MQ=255) gCTTCCTCCCGCTCGCACGCTGCACTCTCGTTTCGTCAATGAACTGGCGCGGCGTGACGACTGGCGTGGCTTGTTTGCCTTTAGCCCGGGAAA‑GCCCCGAACTACCGCAGCAC‑CATGTAATTACTACTATGCAAAAAGGGAAACCGGGCAGAGTGAAGAAGCCCGGAATGGGGCGAAAGAGCTGTGGCAAACCAGCAAATGCCCACCTAACGCCTGTGTCAAGTGATTTAGAGACTGgcgtgcggccggg > 2:192812/1‑244 (MQ=255) tcctccCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCCAAAAGGAACACCGGGCCGAGTGAAGAAGACTGGCACGGGGAGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCCAACGCCTGTGACAAGTTATTTAGTGTCTGgcgggggtaaggaaaac > 2:457390/1‑237 (MQ=255) tcctccCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTaaa < 1:166143/250‑1 (MQ=255) cctccCGCACGCACGCTGCAATCTCGTTTCGTCAAGGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACa < 1:229383/251‑1 (MQ=255) ctccCGCTCGCACGCTGCAATATCGTTTCGTCAATGAACTGGCGCGGCGTGAAGTCTGGCGAGGCTTGTTAGCCTTTAGCCCGGCAAA‑GGCCGGGACTACCGAAGCGC‑CATGTAATTTCTACTATGTAAAATGGAACCCCGGGCAGGGTGAAGAAACCTGGCAAGGGGCGCAAGGGCTTGGGCTAACCGGCCAGAGCCCGCCAAAAGCCCTTGAATAGTTATTTAGTGTCTgggcggccgccagtcacca > 2:230921/1‑233 (MQ=255) cTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCg > 1:303836/1‑251 (MQ=255) cACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTCGCCTTTAGCCCGGAAAA‑GCCCGGCACAACCCGAGCGC‑AATGTAATTAATACTCTGCAACATGGCACCCCGGGCAGAGGGAAGAAGCCTGGCAAGGCGCGAAAGAACTGTGGGTACCCCGGAAGAGCCAGCCTCACGACTGTGACACGTTATTTAATGTCTTGGGTGCGTCAGGTCAACAAGTTcccgtgg > 1:34970/1‑246 (MQ=255) cTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCCTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGGGAAGAAGCCTGGCAAGGGGCGAAGAAACTGTGGCTAACCGGCAAGAGCCAGCCTACCGACTGGGACAAGTTTTTTAGGGGCTGGCGGGCGGCAGGGAACAAAGATCCAGTGGCGtg > 2:346872/1‑250 (MQ=255) cAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAACCTTTAGCCCGGAAAA‑GCCCGGAACTACCCAAGCGC‑AATGTAATTACTACTATGCAAAATTGGACACCGGGCAGAGGGAAATAGCCTGGCAAGGAGCGAAAGAGATGTTGGTAACAGGCAAGAGCCAGCCCAAAGACTGTGAAAAGTTATTTAGTGTATGGGGTGCGGCAGGTAAACAAGATCAGCTGGCGtcatt > 1:363077/1‑247 (MQ=255) aaTCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGGGC‑AATGGAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGGCTGGCCAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCCGCCTAACGCCTTGGACAAGTTATTTAGTGTCTGGCTGGCGTCAGGGAAACAAGGTCCGCTGGccgtttt > 2:359457/1‑244 (MQ=255) cGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGc < 1:322175/250‑1 (MQ=255) cGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGGGCGTCAGGTAAACAAGATCCGCTGGCGTATTTaga < 2:462772/248‑1 (MQ=255) cAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCGGGCCAGGGGCGAAAGAGCTGTGGCTAACCAGCCAGAGCCAGCCTAAAGCCTGGGACAAGTTATTTAGTGTCTGGCGGGCGGCCGGGAAACAAGATCCGCGGGCGTATTTTGAGCGGATCCggc > 2:362586/1‑249 (MQ=255) aaTGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTAGGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCt < 2:211951/251‑1 (MQ=255) gcgcGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGaa < 1:270817/251‑1 (MQ=255) cgcgGCGTGAAGCCTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGGGAAGAAGCCTGGAAAGGGGCGGAAGAGCTGTGGCTAACCGGCAAGAGCCAGGCTAACCCCGGGGGCAAGGTATTTAATGTTCGGCGTGGGTCCGGTAAACAAGGTACGCTGGCGTCTTTTGATCGTAGCCgcgcagcgattaa > 2:85779/1‑238 (MQ=255) gCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACGATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGcc < 2:155982/251‑1 (MQ=255) gTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTg < 1:360772/251‑1 (MQ=255) ggCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAATCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGt < 1:109584/251‑1 (MQ=255) cTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGCGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTa > 1:310795/1‑250 (MQ=255) gTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACa < 2:235764/250‑1 (MQ=255) ttAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGt > 1:361080/1‑251 (MQ=255) ttAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggc < 2:361080/250‑1 (MQ=255) aGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggcagg > 1:154005/1‑251 (MQ=255) cGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTggcagggc > 1:404282/1‑249 (MQ=255) gAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAt < 1:177446/251‑1 (MQ=255) ccctgAACTACCGAAGCGC‑AATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCGGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGAtgcctgc < 2:34337/247‑1 (MQ=255) cTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTAc > 1:319224/1‑251 (MQ=255) ccGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGAGGCCTGCCGATTAca > 2:458308/1‑248 (MQ=255) gcgc‑AATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTAt < 1:40670/251‑1 (MQ=255) aTGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGCTTACCAGACTATCGCCt > 1:31547/1‑251 (MQ=255) ttACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCa > 1:50377/1‑250 (MQ=255) actactAGGCAAAATGGAACCCCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGTGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACTGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAAt < 2:58784/250‑1 (MQ=255) cGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTg > 1:62487/1‑251 (MQ=255) | TGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTGTGACAAGTTATTTAGCGTCTGGCGTGCGTCAGGTAAACAAGATCCGCTGGCGTATTTAGAGCGTATCCGTCTGGCGATGAAAGCGGGTAACACAGGCCTGGTAACAGTGCTGGCAGGGCAGATGCCTGCCGATTACCAGACTATCGCCTCGGCAATCATTTCACTG > NC_000913/4630899‑4631395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |