| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,630,921 | G→A | P63P (CCG→CCA) | slt → | soluble lytic murein transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,630,921 | 0 | G | A | 94.7% | 154.1 / ‑0.6 | 61 | P63P (CCG→CCA) | slt | soluble lytic murein transglycosylase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (29/25); total (31/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-01 | |||||||||||
ATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCGAAATGGAACACCGGGC > NC_000913/4630674‑4631162 | aTAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACtt > 1:322880/1‑251 (MQ=255) aaTCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTAt < 1:187957/251‑1 (MQ=255) cTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGGGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTCACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGGCGAACAAATGATGCCTGGACTGAAAGCTTATACACTTTATccc > 2:179769/1‑251 (MQ=255) cTATTACGCGGCATGACGCTGCATTGATGAATTTACCCTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGAGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATACTCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGACAGATTATCCACTTTATccc > 1:204057/1‑251 (MQ=255) cgGCATGACGCTGCATTGGTGAATTTACACTTGGAGGATACGCTTGTGTAAAAAGCCAAACACGTTACCTAGAGACTGTTGGCTGACGGTGTCTGACTGCTGCAGGTAAGCAGCGTGGCGCGAACCGACTCAGCGGCTGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGAGCTAATCGACTAAATG‑ATGTGGTAGAGCAACTGGTGCAACGAAGGAAAGATTATCCACTTTATCCCTATCTga > 2:55373/1‑249 (MQ=255) cTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCgtc > 1:396692/1‑247 (MQ=255) ttAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCCCTGGATGAGCAGCGTAGCCGTTACGCGCAAATCCAGCGGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCGGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCtgatga > 1:452649/1‑250 (MQ=255) agGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCa > 2:118450/1‑250 (MQ=255) gATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAAcc > 1:471795/1‑251 (MQ=255) cgcTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACcggcg < 1:282237/251‑1 (MQ=255) tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGt < 1:98517/249‑1 (MQ=255) gtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCAc > 1:377925/1‑221 (MQ=255) gtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCAc < 2:377925/221‑1 (MQ=255) tgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGAct > 2:153390/1‑250 (MQ=255) aaaaGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCa < 1:330521/251‑1 (MQ=255) aaaaGCCAAACAAGATAACTGGCGGCTGTTGGCTGACGGTGTATGTCTGCTGACGGTAAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGAAAATCAAGCAGGACTGGGATAATAGAC‑AACTGGAGGTGGGCGAACAAATGATGACTGGACTGAAAGATTATCCACTTTATCCCTAACTGGAATACCGCCAGATAACCGCTACACTGATGAATAAACCGGCGGGGActttc > 2:448577/1‑246 (MQ=255) aaaGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCAc > 1:73368/1‑251 (MQ=255) aaGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGTTGAATCAACCGGCGGGGACTGTCAcc > 2:322175/1‑250 (MQ=255) tACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCATCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAACCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑ATATGGATGAGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGTATACCGTCAACTCACCGAGAACCTGATTAATCAAACGACGGTGAccttaatgaaattttttctct > 2:364007/1‑231 (MQ=255) cTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCt > 1:462772/1‑250 (MQ=255) ggcggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAc > 1:269812/1‑251 (MQ=255) ggggcTGTTGGCTGCCGGGGTCTGTCTGTTGACGGTCAGAAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTCCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAAcc < 2:330387/249‑1 (MQ=255) ggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAAcc < 1:230161/214‑1 (MQ=255) ggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAAcc > 2:230161/1‑214 (MQ=255) ggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGAGGTGACAGTAACTAACTTTTTTCGCCCTAAcccc > 1:107477/1‑251 (MQ=255) gTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACg < 2:133864/250‑1 (MQ=255) tGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCCCTAACTTTGTTCTCGCTAACCCCACgatt > 2:137657/1‑248 (MQ=255) gggCTGCCGGTGTCTGGCGGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCtt < 2:361529/250‑1 (MQ=255) tctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGc > 1:235764/1‑251 (MQ=255) aCGGTCAACAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGACGTGGTCGAACAAATGATGCCTGGACTGAAGGGTTATCCGCTTTATCCCTACCTGGAAAACCGCCAGATCACCGATGATCAGATGAATCAACCGGCGGTGACGGTCAATAACTTTGATCACGCTAACCCCACGCTTCCTCCCGCTCGCCCGCTGCAAtc > 1:111233/1‑251 (MQ=255) ggTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAtctc > 1:155982/1‑251 (MQ=255) gTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCg > 1:211951/1‑251 (MQ=255) gagTGGCGCGAGCCGAGCCACTGGAGGTGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTTAAAGATTATCCTCTTTACCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACATTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTc < 2:506566/249‑1 (MQ=255) gAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGGGACTGGCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTg > 2:21120/1‑251 (MQ=255) gACTCACTGGATGAGCAGCGTAGTCGTTATGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGTCGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTccc > 2:23962/1‑210 (MQ=255) gACTCACTGGATGAGCAGCGTAGTCGTTATGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTccc < 1:23962/210‑1 (MQ=255) gACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGAGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGAGACCGTCACTAACTTTGTTCGCGCTAACCCCCCGCTTCCTCCCGCCCGCCCGCTGCAATCCCGTTTCGTCAATggaacggcgcg > 2:462864/1‑241 (MQ=255) agcagcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTgg < 1:199320/251‑1 (MQ=255) gccGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGGCTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTg < 2:374047/249‑1 (MQ=255) cgcAAATCAAGCAGGCCTGGGATAAGCGAC‑AAATGGATGTGTTCGAACAAATGATGCCTGGACTGAAAGATTATCCCCTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAGCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCttt < 2:281841/251‑1 (MQ=255) gcAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTa < 1:166867/251‑1 (MQ=255) cAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGaaaa < 1:153390/251‑1 (MQ=255) gCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑Gcc < 1:420969/251‑1 (MQ=255) ccTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAACGATGCCTGGACTGAAAGATTATCCACTTTAGCCCTCCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCTCGCTGCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑Gccc < 2:204057/251‑1 (MQ=255) gtggagaaTCGAC‑AAATGGATGTGGTCGAACAAAGGATGCCTGTACTGAAAGATTAGCCACTTTATCCCTACCTGTAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTGCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCgg < 2:258358/245‑1 (MQ=255) aTAATCGAC‑AAATGGATGTGGTCGAACAACTGATGCCTGGACTGAAAGATTATCCACTTTCTCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTTACTTTGTTCGAGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCACTCTCGTTTCGTCCATGAACTGGCGCGGCGGGAAGGCTGGCGTGGCTTGTTTGGCCTTT‑TCCCGGGAAA‑GCCCGGagcc > 2:493050/1‑248 (MQ=255) aTAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACt < 1:493050/251‑1 (MQ=255) cGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCg < 1:137657/250‑1 (MQ=255) aaaTGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGGATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAgcg < 2:172334/251‑1 (MQ=255) cGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCGGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATtact < 2:53257/251‑1 (MQ=255) cGAACAAAAGATGCCTGGACTGAAAGATTATCCCCTTTATCCCTTCCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATtact < 2:267482/251‑1 (MQ=255) tgCCAGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTATCCCCACGCTTCCCCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGcaaa < 2:442337/250‑4 (MQ=255) ggCCTGGACTGAAAGATTAGCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCTCTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACGTGCGCGGCGTGAATACTGGCGTGGCTTGTT‑AGTCTTGAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCaaaa < 1:62715/250‑1 (MQ=255) ccTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑ACCCGGAACTACCGGAGCGCCAAT‑TAATTACTACTATGCAAAATg > 1:63547/1‑251 (MQ=255) cTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGAGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑TGGCTTTAGCCCGGAAAA‑GGCCAGCACTACCGGAGCGC‑ACTGTAATTACTACTATGCCAAATgg > 2:40670/1‑251 (MQ=255) gACTGAAAGATTATCTACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTACCCCCACGCTTCCGCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGTCTGGCGTGGCTTGTT‑AGCCTTTAGCTCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAac < 2:455632/251‑1 (MQ=255) gACTGAAAGATTATCCCCTTTATCCCTACCTGGAATTCCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTGCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAAGGGAac < 2:455641/251‑1 (MQ=255) gACTGAAAGATTATCCACTTTATCCCTACCTTGAATACCGTCAGATCCCCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACCTTGTTCGCGCTAACCCCACGCTTCCGCTCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAac < 1:364007/251‑1 (MQ=255) gACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTTGCCCGGAAAAACCCCGGAA‑TACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAac > 2:177446/1‑251 (MQ=255) ggaaagATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACGCTGCCTCCCGCTCGCACGCTGCAATCTCGTTGCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGTCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCAAAATGGAACAcc < 2:471795/246‑1 (MQ=255) aTTATCCACTTTATCCCTACCTGGCATACAGTCAGATCACCGATGACCTGATGAATCAACCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGACCTGGCGCGGCGAGAAGACTGGCGTGGCTTGTT‑AGACTTTAGACCGGAAAA‑GCCCGGGACTACCGAAGCGG‑AAAGTAATTAATACTATGCAAAATGGAAAACCgggg > 1:357877/1‑248 (MQ=255) | ATAAATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGAC‑AAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAACCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTT‑AGCCTTTAGCCCGGAAAA‑GCCCGGAACTACCGAAGCGC‑AATGTAATTACTACTATGCGAAATGGAACACCGGGC > NC_000913/4630674‑4631162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |