Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,304 A→G Y73Y (TAT→TAC)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,3040AG90.9% 102.9 / 1.3 45Y73Y (TAT→TAC) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base G (26/14);  total (28/17)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑GGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTAT  >  NC_000913/4630063‑4630540
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             
gCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCacac                                                                                                                                                                                                                                      >  2:82740/1‑248 (MQ=255)
    aCGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGa                                                                                                                                                                                                                                  <  1:445951/251‑1 (MQ=255)
        aGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCGGAATGATCTCTGCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGt                                                                                                                                                                                                                               <  1:44838/250‑1 (MQ=255)
          cgcATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTg                                                                                                                                                                                                                            <  2:39596/251‑1 (MQ=255)
            cATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCCGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTGACCAAACTTGATGtttgtc                                                                                                                                                                                                                          >  2:22853/1‑246 (MQ=255)
             aTCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCt                                                                                                                                                                                                                         >  1:408631/1‑251 (MQ=255)
                 gatcgacgttCCAGCTGTCCGTTCAGGTTATGCCCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGGCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCgg                                                                                                                                                                                                                     <  2:416717/242‑1 (MQ=255)
                     cacgctccggaggtacggtcaagttattaCCGGCGTGAGCCGGAATGATCTCTTCCAGTCGGCCTTGGTCAGCGTCCAGCTTGTCAAAACCGGCATCCTGATCGGCGTACATCGCATCCACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGTTTTGTTCCGGGTTCAGCTGCGGTTCCTGCTGCAGGTCACCAATCTCGATGTCTGGCTGCGGAcgc                                                                                                                                                                                                                 <  2:19154/228‑1 (MQ=255)
                      aCGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGCAACCACTCTTTATGTCTGGCTGCGGaggtc                                                                                                                                                                                                                >  1:416717/1‑247 (MQ=255)
                               cTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAAt                                                                                                                                                                                                                                       <  1:391254/219‑1 (MQ=255)
                                   ccGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGAt                                                                                                                                                                                                   <  1:127803/250‑1 (MQ=255)
                                     gTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AGCCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGt                                                                                                                                                                                                 <  1:424949/251‑1 (MQ=255)
                                     gTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGt                                                                                                                                                                                                 <  1:235584/251‑1 (MQ=255)
                                           gttaggACCGTCGTGAGCCTGAATGATCTCTTCCAGCCGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCttt                                                                                                                                                                                             <  2:138041/244‑1 (MQ=255)
                                           gttATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGGAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCttt                                                                                                                                                                                             >  1:138041/2‑249 (MQ=255)
                                                gACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGGCGCGCTTCACCTTCGTTGTCTTTaaccatg                                                                                                                                                                                      >  2:463027/1‑245 (MQ=255)
                                                              tGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCCCTTCAGCAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCCGGTAACCCATCTTGCTGTCTGGCTGCGGCCCCCCTTCCCCTTCGCTGTCTTTCTCatatcaccccaacatgcg                                                                                                                                                                        >  2:440184/1‑234 (MQ=255)
                                                                          cccAGACGCCTTTGTTCAGCGCCCAGTGTGTAAAAACCGGCATCCGTAGCGGCGTACAGCGCGTACTCTTCACCCAGGTGTTGCAGGGCGTA‑ACCCCCTTCTGAAACCGCTTCTTCAAGGGACTCACGCAGGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATACCCAATCTTGATGTCTGGCTGCGGACGCGTTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑g                                                                                                                                                             <  1:114207/248‑1 (MQ=255)
                                                                              gACGGCTTTGTGCAGGGGCCAGCTTGTCAAAATCGCCAGCCGGATCGTCTTACAGCGCATACACTTCACCCAGGCGTTTCAGGGCTTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGGTACCCATTCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTa                                                                                                                                                       <  1:260506/251‑1 (MQ=255)
                                                                                         tCAGCGGCCAGCTTGGCAAAATCGGCAGCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGGTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGCCTCACGCACGGTGGGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGAAACCCATCTTGATTTCTTGCTGCGGTCGCGCTTCACCTTCGGAGGCTTTTTCAATGCCCGCCCTAAGGCGCAGCATGGtaggctttcacgcgccgtt                                                                                                                                             >  2:410233/1‑233 (MQ=255)
                                                                                              ggCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTCCCCGCGCCGTTCAGAc                                                                                                                                       >  1:301399/1‑251 (MQ=255)
                                                                                              ggCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGAGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGGAACCAATCTTGATATCGGGCTGCGGACGCGCTTCACCTTCAGTGTCTTTATCAATTCCCGCCATAATGCACAGCAGGAT‑AGACTTACCCGCGccgttaaga                                                                                                                                        >  1:271356/1‑243 (MQ=255)
                                                                                                ccAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGCCTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGGCGCGCTTTCCCTTCGTGGTCTTTATCCATGCCCGCCCTAATGCGCAGCAGGGG‑AGACTTTCCCGCCCCGTTCCGAccc                                                                                                                                     >  2:169254/1‑251 (MQ=255)
                                                                                                  aGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCgcag                                                                                                                                                                     >  1:401645/1‑218 (MQ=255)
                                                                                                    cTTGTCAAAATCGGCATCCGGATCGGAGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGGAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCCGGGT‑AGACTTACCCGCCCCGTTCAGcccccgg                                                                                                                                  >  2:57670/1‑247 (MQ=255)
                                                                                                                  cATCCGGATCGGCGTACAGCGCATACACTTCATCCACACGTTTCAGGGCGTT‑AACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCCCGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCCATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑GGCCTTACCCGCGCCATTCAGACCCAGGACACCACTTTTTGcc                                                                                                                   >  1:213217/1‑251 (MQ=255)
                                                                                                                       ggATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTTCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAgg                                                                                                              >  1:119721/1‑251 (MQ=255)
                                                                                                                       ggATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAgg                                                                                                              >  1:162678/1‑251 (MQ=255)
                                                                                                                               gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGGAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATTAATGCCCGCCATAATGCGCAGCAGGGT‑AGGCTTACCCGCGCCGTTCAGACCCAGGGCCCCCATTTTTGTCCCAGGGGAGaaa                                                                                                       >  2:137423/1‑250 (MQ=255)
                                                                                                                                             cTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGttt                                                                                        >  1:181490/1‑251 (MQ=255)
                                                                                                                                                    ccggtcgttCAGGGCGTTTAACCACTTAAGAAACCCCTTCTTCAAGGCCCCCCCGCAGGTTGTGTCCCGGGTGCAGCTGCGGTTCCTGCGGCAGGTAACCAATTTTGATGTCGGGCGGCGGACGCGCTTCCCCTTGGATGTCTTTACAAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCa                                                                                    <  1:82740/242‑1 (MQ=255)
                                                                                                                                                      ggCGTTTCAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATTTCTGGCTGCGGACGCGCTTCACCTTCGCTGGCTTTTTCAATGCCCGCCCTAATGCGCAGCAGGGT‑AGACTTAACCGCGCCCTTCCGACCCACGACACCAATTTTTGCCCCCGGGAAGAAACTACGAGGGATGTTTTTccacat                                                                                >  2:49148/1‑245 (MQ=255)
                                                                                                                                                             cAGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATTCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGtt                                                                        >  1:506566/1‑251 (MQ=255)
                                                                                                                                                              aGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCgggcg                                                                                                                                                                                                                  >  2:131312/1‑110 (MQ=255)
                                                                                                                                                              aGGGCGTT‑AACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGAcg                                                                                                                                                                                                                  <  1:131312/113‑1 (MQ=255)
                                                                                                                                                              aGGGCGTT‑AACCACTTATGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCTTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑GGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGttt                                                                       >  2:14570/1‑251 (MQ=255)
                                                                                                                                                                  gggttaccctcttcagaaactcctccttcAATGGACCCAGCCACGGTTTTTTCCGGGTTCATTGGCGTTTCCTGCGGCAGTTAACCATTCTTGTTGTCTTGCTGCGGCCGCGCTTCACCTTGGTTGTCTTTATCAATCCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTcgg                                                                    <  1:348520/226‑1 (MQ=255)
                                                                                                                                                                          cACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCCATCTTGATGTCTGGCTGCGGCCGCGCTTCACCTTCGATGTCTTTATCAATTCCCGCCCTAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGacca                                                             >  2:270314/1‑247 (MQ=255)
                                                                                                                                                                                      cGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGCAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGCAGAAAACCGGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTTCCggacc                                                >  2:438606/1‑247 (MQ=255)
                                                                                                                                                                                                     cTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCACGTTCCCAATCTTGTTGTTTGGCTCCGGGCGCGCTTCACCTTCGCTGACTTTATCAATGCCCGCCATACTGCGCAGCAGGGT‑AGACTTACCCGCGACGTTCAAGCCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGAAGTTTTTCAAAATATTACGTTTCGGCGGAACAAACTTGCCGGCACGATGCATGGTATaa                                  >  2:46262/1‑250 (MQ=255)
                                                                                                                                                                                                           cacgGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGGAACCAATCTTGATGTCTGGCTGCGGGCGCGCTTCACCTTCGTTGTCTTTATAAATGCCCGCCATAATGCGCAGCAGGGTAGGA‑TTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTATGAGAGATGTTTTTCAAAAATTGACGTTTCGGCGGAACAAATTTGGCGAAACGAAGCATGGTATAAAcagagt                           >  2:305138/1‑246 (MQ=255)
                                                                                                                                                                                                              ggTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGGCTGGCTGCGGACGCGCCTCCCCTTCGCTGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGG‑AGACTTCCCCGCGCCGTTCAGCCCCAGGCAACCAATTTTTGCCCCAGGGAAGACACTCAGAGAGATGTTTTTCAAAATATGTCGCTTCGGCGGAAAAACTTTGCCGTCACGCTGCATGGAATAAACCAAtttgg                        >  2:183563/1‑248 (MQ=255)
                                                                                                                                                                                                                            ccgtcTGCGGTTCCTGCGGCAGGTACCCATTCTTGATTTCTGGCTGCGGGCGCTCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGTGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACtt           <  1:466462/246‑1 (MQ=255)
                                                                                                                                                                                                                                    ggTTCTTGCGGCAGATACCCAATCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑GGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTAt  <  1:14570/251‑1 (MQ=255)
                                                                                                                                                                                                                                    ggTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑AGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGGATAAACGAATTGATGCACGTTGGACTTCGCCTCTAt  >  1:281841/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             
GCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTT‑AACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGT‑GGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTAT  >  NC_000913/4630063‑4630540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑