Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,933	G→A	L343L (CTA→TTA)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,933	0	G	A	100.0%	72.7 / NA	27	L343L (CTA→TTA)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/8);  total (19/8)

TGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTT  >  NC_000913/6693‑7176                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     gggTAATAGCCAGGCAGGCCAGTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACTATGGAGCTGAAGGCAAACAGAATAACCACGATGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATc                                                                                                                                                                                                                                            <  2:182688/250‑1 (MQ=255)   gTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGc                                                                                                                                                                                                                                          >  1:377286/1‑251 (MQ=255)    tAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGcc                                                                                                                                                                                                                                         >  1:60518/1‑251 (MQ=255)        aGCCATGCAGGACATAATGATATCTGCCAGTTGCCACATCAGCGGAAGTCATAGCAAGGTGACGCCGATGGACGTTGCGAAGGTGCAGATCCGCAAACCCCAGATCGCTTTAGGGTTGTTCAGGCGTAGAAAGAAGAGAATGTTTTCCGCGTAAATGTT‑GCTGGCAACGATGGAGCTGAAGCCATACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGAAACACATTAACACCCGCAtagacctcc                                                                                                                                                                                                                                     >  1:455527/1‑243 (MQ=255)                  gCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTg                                                                                                                                                                                                                           <  1:269406/251‑1 (MQ=255)                        aTGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACCCCCGCATCGCCTTCTGGATAAGCTGAATAc                                                                                                                                                                                                                      <  1:258388/250‑1 (MQ=255)                                 gCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCgg                                                                                                                                                                                                            >  2:190732/1‑251 (MQ=255)                                         ccACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCACACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGACTTCTGGATAAGCTTAATACCTTCCAGCGGCATGTAgg                                                                                                                                                                                                    >  1:114751/1‑251 (MQ=255)                                              tCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCCTGTAGGTtgtg                                                                                                                                                                                               >  2:388151/1‑251 (MQ=255)                                                agaggaagaattAGCAAGGGGCCGCCGATGACCGTTGCGAAGGTGAAGATCCGCAAACACCAGAGCGCTTTAGGGTGGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAAGAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTGCCAGCGGCATGTAGGTTGTGcc                                                                                                                                                                                             <  2:207718/241‑1 (MQ=255)                                                                  gTGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCACACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGAAatat                                                                                                                                                                           >  2:66731/1‑251 (MQ=255)                                                                                             cAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGtt                                                                                                                                                                                                  <  1:333157/201‑1 (MQ=255)                                                                                                   cgaaaaCACCAGTTCGTTTTAGGGTTGTGCAGAGGAAAAAAGAGAAGTTTGTTTGCGGC‑GAAAAGTATGTTGGCAACGAGGGAGCGGAAGGAAAACAGAATACCCACGAGGGAAACGACCCCAGCCCCCCAGGAACCCATAAACCCCCGCATCGCCTTCGGAAAAAGCTGAATCCCTCCCAGGGGCATGTAGTTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGgt                                                                                                                                          <  1:183454/248‑1 (MQ=255)                                                                                                             aGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaa                                                                                                                                 >  1:188676/1‑250 (MQ=255)                                                                                                              gATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGAAGGTTGTGCCGTTCCCCGCCAGTAATATCAACAAGGCGCTTGCCGAAAAGGTGACCAGGGTGTCGTTaaaa                                                                                                                                >  2:241154/1‑250 (MQ=255)                                                                                                                                 aGGCGTAAATAGCAGAGATTGTTTTCGGCGTAATTGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAACCTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTTCCGTAACGATGACCAGGGTGTCGATCAAAATGCCAATCATCTggccac                                                                                                             >  2:34828/1‑246 (MQ=255)                                                                                                                                           agaagaGATTGTTTTCGGCGTAACTGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGGGTCGATAAAAATGCCCATAATCTGGACACTCCCTTGCGCt                                                                                                  >  2:454718/1‑251 (MQ=255)                                                                                                                                                                     tA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCGCCCCAGGAACCCATTAACACCCGCATCGCCTTATGGATAAGATGAATACCTTCCAGCGGCATGAAGGTTGGGCCGTTACCCGCCAGTAAAATCAGCATGGCGCTTTCCGTCAAGATGACAAGGGTGTCGATAAAAATGCCAAAAATCTGGAAAATCCCTAGCGCTGCCGGAGTG‑GGAGGCCAAGAcgccgc                                                                       >  2:344785/1‑251 (MQ=255)                                                                                                                                                                                   tGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCt                                                                                                                   >  2:438313/1‑195 (MQ=255)                                                                                                                                                                                          gAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGGACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCCGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGACGATAAAAATGCCAATCATCTGGACACACCCTTGAGCTGCCGGA‑TGCGGAGGCCCAGACACCGCTGCCGCGCCCTCGTTTGGCg                                                   >  2:164531/1‑251 (MQ=255)                                                                                                                                                                                                    aGAATAACCACGAGGGTAAAAAACTCAGCACCCCAGAACCCCATAAACCCCCCCATCGCCTTTTGGATAAGCTGAATCCCTCCCAGCGGCATGTAGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCAt                                         <  1:388151/251‑1 (MQ=255)                                                                                                                                                                                                          aCCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTggaggtcggaagagcgtcgg                                   >  2:178148/1‑233 (MQ=255)                                                                                                                                                                                                                   gTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTg                                                                                                                                                                                                                                    <  2:153424/50‑1 (MQ=255)                                                                                                                                                                                                                   gTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTg                                                                                                                                                                                                                                    >  1:153424/1‑50 (MQ=255)                                                                                                                                                                                                                         aaCTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGCCAATCCCTTGCGCTGCCGGA‑GGCGGAGGCCAGGAAGCCGCTGCCGCCGCCGCGTTTGGCGTCGACCCCATTCCCACCTCATTGGAAAAcc                     >  2:433510/1‑249 (MQ=255)                                                                                                                                                                                                                                      aGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCCGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGACAACCTACTGCGCTGaac        >  2:178893/1‑249 (MQ=255)                                                                                                                                                                                                                                             cATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGACGTTAAAAATGCCAATCATCTGGACAATCACTTGCGCTGACGGG‑AGAGGAGGCCCGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCCTTGGaaacaaaactgagcttcaaaccagt  >  2:199991/1‑227 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     TGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTT  >  NC_000913/6693‑7176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑