Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,446	A→G	G359G (GGT→GGC)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,446	0	A	G	100.0%	132.6 / NA	45	G359G (GGT→GGC)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/15);  total (30/15)

CGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGA  >  NC_000913/4629199‑4629692                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       cgcgTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCg                                                                                                                                                                                                                                                     <  1:473017/251‑1 (MQ=255)         cccTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAACCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGAcc                                                                                                                                                                                                                                             >  1:466632/1‑251 (MQ=255)                         taaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGAt                                                                                                                                                                                                                              <  1:93702/250‑1 (MQ=255)                          aaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGCGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTACCCAAACGGGTTTGCTGTTATCAATTGAGTCACGGAACTGATCAACCGATGCCAGTTTAACCGTTTCACCTACAGGGCTGGTGACGCTGTCCGGCTGTTCCTGCCCCGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGGTCGGACCGATGGTccacacgtttg                                                                                                                                                                                                                            >  2:164536/1‑242 (MQ=255)                           aagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGCCCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGGTCGGACCGATGATCCcgacgcttgc                                                                                                                                                                                                                           >  2:380945/1‑246 (MQ=255)                                aaagCGGCCAACGAAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGGTTTGCTGTTATCAATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGGGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCtt                                                                                                                                                                                                                      >  1:58307/1‑251 (MQ=255)                                     ggCCAACGTAGGCGCGGCTTGGCATCTCGGAGTTGCCGATCTTCATAATATACAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTACAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGGTTTACAAGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCttaccgg                                                                                                                                                                                                                 >  1:7805/1‑246 (MQ=255)                                                gcgcGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCt                                                                                                                                                                                                      <  2:432432/251‑1 (MQ=255)                                                      gttggcgtatcGGTGTTTCCGAGCTTCAAAACATCCGCCCCGCCGGAATCTTCTGCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAGCTGGTCAACCGATGCCAGTTTCACCGTTTCGCCTAAAGGGATGGTGCCGCTGTCCGGCTGTTCCTGCCCAGAGATCATACGGAACAGGGTCGATGTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc                                                                                                                                                                                                <  2:225078/242‑1 (MQ=255)                                                        tGGCATCTCGGTGTTGCCGATCTTCTTAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTGATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc                                                                                                                                                                                                <  2:336928/249‑1 (MQ=255)                                                        tGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc                                                                                                                                                                                                <  1:379551/249‑1 (MQ=255)                                                           cATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtcatca                                                                                                                                                                                            >  1:38723/1‑250 (MQ=255)                                                                 ggTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagca                                                                                                                                                                                     <  1:120550/251‑1 (MQ=255)                                                                    gtTGCCGATCTTCATAATAGCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGAGGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGGCGATTTACCCTCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagcagg                                                                                                                                                                                   <  1:453668/250‑2 (MQ=255)                                                                                                  ggAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTg                                                                                                                                                     >  1:33791/1‑250 (MQ=255)                                                                                                                               tATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGTTTGATGCCTTCCAGCACTTTATCGCACAGAcgcgtt                                                                                                                       >  1:480680/1‑249 (MQ=255)                                                                                                                                 tCCATTGAGTCACGGAACTGCTCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGCTGATCCCGACGATTGCCCCTTTCGGGATCGCGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTCGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGCCGCGGTcc                                                                                                                     >  2:45226/1‑251 (MQ=255)                                                                                                                                  ccATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTcc                                                                                                                     >  1:505314/1‑250 (MQ=255)                                                                                                                                   cATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTcc                                                                                                                     >  1:92282/1‑249 (MQ=255)                                                                                                                                       gAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTgg                                                                                                               >  1:216284/1‑251 (MQ=255)                                                                                                                                              ggAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGGTGCTGACTTCCAGCACTTTCACCCCCCGACGCGGTCCAGGGGGACTAAAc                                                                                                        >  2:348897/1‑251 (MQ=255)                                                                                                                                                aaCTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAAc                                                                                                        >  1:377956/1‑249 (MQ=255)                                                                                                                                                     aTCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGt                                                                                                 >  1:500771/1‑251 (MQ=255)                                                                                                                                                          caggtgCCAGTTACACCGGTTCACCTAAAGTGTAGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTGCGGACCGATGATCCCGACGACTGCCCCTTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCCCCGTAGGATTTACGCAGATTGCTGACTTCCAGCATTTTATCGCCCATACGCGGTCCAGGTGGAATAAACAGTTCGTTGGtt                                                                                            <  2:161831/246‑1 (MQ=255)                                                                                                                                                             gggcaattttacccgttgcaccAAAAGGGATGGTGCCGATGTCCGGCTGCTCCTGACCAGATATCATTCGGAACAGGGACGATTTACCCGCGCCGTGCGGACCGAGGATCCCGACGAGTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCTGTCCAGGTGGAATAAACAGTTCGTTGGttgcg                                                                                         <  2:189775/239‑4 (MQ=255)                                                                                                                                                                         ccgtttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTg                                                                              >  1:72305/1‑250 (MQ=255)                                                                                                                                                                           gtttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTc                                                                                                   <  1:114245/227‑1 (MQ=255)                                                                                                                                                                           gtttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTc                                                                                                   >  2:114245/1‑227 (MQ=255)                                                                                                                                                                            tttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTT‑ATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATAtt                                                                         >  1:207051/1‑251 (MQ=255)                                                                                                                                                                                  cgaacgTGATGGTGCCGCTGTCCGGCTGTTACTGACCAGAGATCATACGGAACAGGGTCGGTTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCCATAAGCAGGCGATCACCGTAGGATTTACGCCGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGAGGTCCAGGTGGAATCAACAGCTCGTTTGTTTCGTTAAGTTTATGGTATTCGGTg                                                                    >  2:261432/6‑251 (MQ=255)                                                                                                                                                                                      aGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGt                                                                >  1:183884/1‑251 (MQ=255)                                                                                                                                                                                          aTGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGTTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCGAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTc                                                              >  2:64163/1‑249 (MQ=255)                                                                                                                                                                                                     gTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCaaa                                                   <  1:398133/249‑1 (MQ=255)                                                                                                                                                                                                          gCTGGTCCTGACCAGAGATCAGACGGAACAGGGTCGATTTACCCGCGCCGTGCGGACCGATGATCCCGACGAGTGCCCCGTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGTTTGCTGACTTCCAGCACTGTATCGCCCAGACGCGGGCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCGCTTCAAAGCGTGcc                                            <  2:428842/251‑1 (MQ=255)                                                                                                                                                                                                            tGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGcc                                            >  1:410274/1‑249 (MQ=255)                                                                                                                                                                                                            tGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTAACCGCGCCGTTCGGACAGATGATCCCGACGATTGCCCCTTTCGGGCACGAGAAGCTCAGGTCATCAATCAGCAGGCGCTCACCGTAGGCTTTACGCAGGTCGCTGCCTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTAGGTTTATGGTATTCGGTGATGTTCAGCTCTTCACAGCGGGcccg                                          >  2:504792/1‑249 (MQ=255)                                                                                                                                                                                                                       agagATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCACAGCGTGCCAGACGTGCTTTAc                               >  2:10383/1‑251 (MQ=255)                                                                                                                                                                                                                       agagATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTAc                               >  2:232769/1‑251 (MQ=255)                                                                                                                                                                                                                          gaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCtt                            >  2:137900/1‑251 (MQ=255)                                                                                                                                                                                                                          gaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCtt                            >  2:97315/1‑251 (MQ=255)                                                                                                                                                                                                                             cATACGGACCAGGGTCGATTTACCCGCGCAGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGAGTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTGCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCGTTc                          <  2:38723/250‑1 (MQ=255)                                                                                                                                                                                                                              aTACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAGGCGTGCCAGACGTGCTTTACCTTTCg                         >  2:348287/1‑250 (MQ=255)                                                                                                                                                                                                                               tACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCCGGATCGAGCAGCTCAGGTCATCAATCAGCAGGCGATCAACGTAGAATTTACACAGGTTACTGACTTCCAGCACTTTATCGCCCAGACGCGGACCAGGTGGACTAAAAAGATCGTTGGATTCGTTCCGTTTCTGATATTCGGTGATGTTCAGCTCTTCAAAGCGTGCACGACGTGCTTAACCTTTCGAc                       >  1:58133/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaCAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTggcg                  >  1:294896/1‑248 (MQ=255)                                                                                                                                                                                                                                                    ccGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAAGCAGCAGGCGATCCCCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGtgccctga  <  2:72305/251‑8 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       CGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGA  >  NC_000913/4629199‑4629692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑