Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,898	A→G	L354L (CTT→CTC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,898	0	A	G	100.0%	80.8 / NA	27	L354L (CTT→CTC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/10);  total (17/10)

GTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGC  >  NC_000913/6653‑7143                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gtagtaaCCACAGGCGAGAGCATTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGt                                                                                                                                                                                                                                                   <  2:122347/246‑1 (MQ=255)           caGGCGAGAGCAGGAAAATGGCGGGCAAATTGGGAATAGCCAGGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGACCGCTTTAGGGTTGTTCAGGCGGAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCa                                                                                                                                                                                                                                         <  2:278307/251‑1 (MQ=255)                    gCAGTAAAATGGCGGTCAAATTGGTAATAGCCCTGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGGAGGGGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GGTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACGAACTCAGcccccc                                                                                                                                                                                                                                  >  2:183454/1‑249 (MQ=255)                         aaaaTGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAAcc                                                                                                                                                                                                                           <  1:218163/251‑1 (MQ=255)                              ggtggTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTACATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACACCCGGAACCCAtt                                                                                                                                                                                                                       >  2:153228/4‑250 (MQ=255)                                         gggTAATAGCCAGGCAGGCCAGTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACTATGGAGCTGAAGGCAAACAGAATAACCACGATGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATc                                                                                                                                                                                                           <  2:182688/250‑1 (MQ=255)                                           gTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGc                                                                                                                                                                                                         >  1:377286/1‑251 (MQ=255)                                            tAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGcc                                                                                                                                                                                                        >  1:60518/1‑251 (MQ=255)                                                aGCCATGCAGGACATAATGATATCTGCCAGTTGCCACATCAGCGGAAGTCATAGCAAGGTGACGCCGATGGACGTTGCGAAGGTGCAGATCCGCAAACCCCAGATCGCTTTAGGGTTGTTCAGGCGTAGAAAGAAGAGAATGTTTTCCGCGTAAATGTT‑GCTGGCAACGATGGAGCTGAAGCCATACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGAAACACATTAACACCCGCAtagacctcc                                                                                                                                                                                                    >  1:455527/1‑243 (MQ=255)                                                          gCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTg                                                                                                                                                                                          <  1:269406/251‑1 (MQ=255)                                                                aTGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACCCCCGCATCGCCTTCTGGATAAGCTGAATAc                                                                                                                                                                                     <  1:258388/250‑1 (MQ=255)                                                                         gCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCgg                                                                                                                                                                           >  2:190732/1‑251 (MQ=255)                                                                                 ccACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCACACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGACTTCTGGATAAGCTTAATACCTTCCAGCGGCATGTAgg                                                                                                                                                                   >  1:114751/1‑251 (MQ=255)                                                                                      tCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCCTGTAGGTtgtg                                                                                                                                                              >  2:388151/1‑251 (MQ=255)                                                                                        agaggaagaattAGCAAGGGGCCGCCGATGACCGTTGCGAAGGTGAAGATCCGCAAACACCAGAGCGCTTTAGGGTGGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAAGAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTGCCAGCGGCATGTAGGTTGTGcc                                                                                                                                                            <  2:207718/241‑1 (MQ=255)                                                                                                          gTGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCACACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGAAatat                                                                                                                                          >  2:66731/1‑251 (MQ=255)                                                                                                                                     cAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGtt                                                                                                                                                                 <  1:333157/201‑1 (MQ=255)                                                                                                                                           cgaaaaCACCAGTTCGTTTTAGGGTTGTGCAGAGGAAAAAAGAGAAGTTTGTTTGCGGC‑GAAAAGTATGTTGGCAACGAGGGAGCGGAAGGAAAACAGAATACCCACGAGGGAAACGACCCCAGCCCCCCAGGAACCCATAAACCCCCGCATCGCCTTCGGAAAAAGCTGAATCCCTCCCAGGGGCATGTAGTTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGgt                                                                                                         <  1:183454/248‑1 (MQ=255)                                                                                                                                                     aGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaa                                                                                                >  1:188676/1‑250 (MQ=255)                                                                                                                                                      gATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGAAGGTTGTGCCGTTCCCCGCCAGTAATATCAACAAGGCGCTTGCCGAAAAGGTGACCAGGGTGTCGTTaaaa                                                                                               >  2:241154/1‑250 (MQ=255)                                                                                                                                                                         aGGCGTAAATAGCAGAGATTGTTTTCGGCGTAATTGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAACCTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTTCCGTAACGATGACCAGGGTGTCGATCAAAATGCCAATCATCTggccac                                                                            >  2:34828/1‑246 (MQ=255)                                                                                                                                                                                   agaagaGATTGTTTTCGGCGTAACTGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGGGTCGATAAAAATGCCCATAATCTGGACACTCCCTTGCGCt                                                                 >  2:454718/1‑251 (MQ=255)                                                                                                                                                                                                             tA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCGCCCCAGGAACCCATTAACACCCGCATCGCCTTATGGATAAGATGAATACCTTCCAGCGGCATGAAGGTTGGGCCGTTACCCGCCAGTAAAATCAGCATGGCGCTTTCCGTCAAGATGACAAGGGTGTCGATAAAAATGCCAAAAATCTGGAAAATCCCTAGCGCTGCCGGAGTG‑GGAGGCCAAGAcgccgc                                      >  2:344785/1‑251 (MQ=255)                                                                                                                                                                                                                           tGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCt                                                                                  >  2:438313/1‑195 (MQ=255)                                                                                                                                                                                                                                  gAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGGACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCCGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGACGATAAAAATGCCAATCATCTGGACACACCCTTGAGCTGCCGGA‑TGCGGAGGCCCAGACACCGCTGCCGCGCCCTCGTTTGGCg                  >  2:164531/1‑251 (MQ=255)                                                                                                                                                                                                                                            aGAATAACCACGAGGGTAAAAAACTCAGCACCCCAGAACCCCATAAACCCCCCCATCGCCTTTTGGATAAGCTGAATCCCTCCCAGCGGCATGTAGGTTGTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCAt        <  1:388151/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTggaggtcggaagagcgtcgg  >  2:178148/1‑233 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGC  >  NC_000913/6653‑7143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑