Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,281	G→A	I414I (ATC→ATT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,281	0	G	A	100.0%	127.8 / NA	41	I414I (ATC→ATT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/20);  total (21/20)

GCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGA  >  NC_000913/4629042‑4629515                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           gCACAGCCCGGGAACTCAAGCAGGCAGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTAACCAACGCGTTTACCCTGATCAACCCATTTAAGGGTACAGCGGCCAACGTAGGCCCGGCTTGGCTACTCGGTGTTGCAGATCTTCCTAATATCCAGccc                                                                                                                                                                                                                                 >  2:252955/1‑251 (MQ=255)           ggAACTCCAGCAGGGCGTTTTCCAGCGCGAGAAGGGATTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTAACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGCAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCGTCCGGAAAct                                                                                                                                                                                                                       >  1:435636/1‑251 (MQ=255)              actccgtcagGGCGTTTTCCAGCGAGCGAAGGGGTTCGATGACCGGGTCGTTGGTGGGTTCGGCGAGCAGCAGCATGTTGCCGCCATCCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCCCACCACCGGAAAGTTCACCAACGCGTTTACCCGGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCTGAAActtct                                                                                                                                                                                                                    <  2:115034/244‑1 (MQ=255)                           gTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGttt                                                                                                                                                                                                       >  1:19014/1‑251 (MQ=255)                               tCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGCAACTTCTTCCCAAACGGTTTTGCt                                                                                                                                                                                                   >  1:342991/1‑251 (MQ=255)                                              gTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGccc                                                                                                                                                                                                                                 <  2:438206/206‑1 (MQ=255)                                              gTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGccc                                                                                                                                                                                                                                 >  1:438206/1‑206 (MQ=255)                                                                    gtgggtcgccgtgcagcatgaTGTTGCCCCCAACCTGCGGCAGCGTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGcc                                                                                                                                                              <  2:168984/231‑1 (MQ=255)                                                                                gagacgacagtttggcgccACCCCGCAGCAGCTGCGCCAGGTGCAGACGACCGCGCTCCCCACCGGAAAGTTCATCAGCGCGTGTACCCTGAGCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCAGTGAGTCACGGAACTGATCAACCGATGCCAgtttcaccgtt                                                                                                                                                  <  2:82749/236‑1 (MQ=255)                                                                                   gcagcaTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTGGAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCTGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCAGTGAGTCACGGAACTGATCAACCGATGCCAgtttcaccgtttca                                                                                                                                               <  2:303339/251‑1 (MQ=255)                                                                                    cagcaTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCACACCACCGGAAAGTTAACCAACGCGTTTACCCTGATCAACCCCTTTAACGTTAAAGCGGACAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAgtttcaccgtttcac                                                                                                                                              >  1:30807/1‑251 (MQ=255)                                                                                             gccgccAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTg                                                                                                                                      >  1:432432/1‑250 (MQ=255)                                                                                                        gcagcaGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACACTGAGCAACCCCATTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCGCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTg                                                                                                                          <  1:312307/251‑1 (MQ=255)                                                                                                             agcTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCgg                                                                                                                     <  1:490259/251‑1 (MQ=255)                                                                                                               cTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCt                                                                                                                   >  1:161831/1‑251 (MQ=255)                                                                                                                ttCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTg                                                                                                                  >  2:398133/1‑251 (MQ=255)                                                                                                                 tCGCCAGATGCAGACCACCGCGCTCACCACCGGAAAGTCCCCCACCGCGTTTACCCTGATCAACCCCTGTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTg                                                                                                                  <  2:9455/250‑1 (MQ=255)                                                                                                                                gacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAACCTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGCTGCCAGTTTCCCCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTgccccg                                                                                                       >  2:93702/1‑241 (MQ=255)                                                                                                                                     cgcTCACCCCCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCGTTAAAGTTAAAGCGGCCAACGGAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTGCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGAGCATACg                                                                                             <  2:461825/251‑1 (MQ=255)                                                                                                                                            caccGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAgg                                                                                      >  2:121775/1‑251 (MQ=255)                                                                                                                                             cccGGAAAGTTCCCCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAATCGGCAAACGTAGGCGCGGCTTGGCATCTCGGGGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAggg                                                                                     <  2:19014/250‑1 (MQ=255)                                                                                                                                                      ttCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGATTTGCTGTTATCCATTGAGTCACGGAACCGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGCGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATAAGGAACAAGGTAGATTTa                                                                             >  2:473017/1‑250 (MQ=255)                                                                                                                                                        cACCAACGCGTTTACCCTGAACATCCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCTCCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTAccc                                                                          <  1:22511/251‑1 (MQ=255)                                                                                                                                                             aCGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGcgcc                                                                     >  1:25967/1‑248 (MQ=255)                                                                                                                                                              cgcgTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCg                                                                    <  1:473017/251‑1 (MQ=255)                                                                                                                                                                cgTTTACCCTGATCAACCCCTTTAACGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCCGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCGGCCCAGAGATACTACGGACCAGGGTCGCTTTACCCGccccatt                                                                  >  2:147681/1‑245 (MQ=255)                                                                                                                                                                      cccTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAACCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGAcc                                                            >  1:466632/1‑251 (MQ=255)                                                                                                                                                                                      taaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGAt                                             <  1:93702/250‑1 (MQ=255)                                                                                                                                                                                       aaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGCGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTACCCAAACGGGTTTGCTGTTATCAATTGAGTCACGGAACTGATCAACCGATGCCAGTTTAACCGTTTCACCTACAGGGCTGGTGACGCTGTCCGGCTGTTCCTGCCCCGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGGTCGGACCGATGGTccacacgtttg                                           >  2:164536/1‑242 (MQ=255)                                                                                                                                                                                        aagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGCCCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGGTCGGACCGATGATCCcgacgcttgc                                          >  2:380945/1‑246 (MQ=255)                                                                                                                                                                                            taaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATAc                                                                                              <  2:261908/195‑1 (MQ=255)                                                                                                                                                                                            taaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATAc                                                                                              >  1:261908/1‑195 (MQ=255)                                                                                                                                                                                             aaagCGGCCAACGAAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGGTTTGCTGTTATCAATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGGGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCtt                                     >  1:58307/1‑251 (MQ=255)                                                                                                                                                                                                  ggCCAACGTAGGCGCGGCTTGGCATCTCGGAGTTGCCGATCTTCATAATATACAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTACAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGGTTTACAAGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCttaccgg                                >  1:7805/1‑246 (MQ=255)                                                                                                                                                                                                             gcgcGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCt                     <  2:432432/251‑1 (MQ=255)                                                                                                                                                                                                                   gttggcgtatcGGTGTTTCCGAGCTTCAAAACATCCGCCCCGCCGGAATCTTCTGCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAGCTGGTCAACCGATGCCAGTTTCACCGTTTCGCCTAAAGGGATGGTGCCGCTGTCCGGCTGTTCCTGCCCAGAGATCATACGGAACAGGGTCGATGTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc               <  2:225078/242‑1 (MQ=255)                                                                                                                                                                                                                     tGGCATCTCGGTGTTGCCGATCTTCTTAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTGATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc               <  2:336928/249‑1 (MQ=255)                                                                                                                                                                                                                     tGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtc               <  1:379551/249‑1 (MQ=255)                                                                                                                                                                                                                        cATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGtcatca           >  1:38723/1‑250 (MQ=255)                                                                                                                                                                                                                              ggTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagca    <  1:120550/251‑1 (MQ=255)                                                                                                                                                                                                                                 gtTGCCGATCTTCATAATAGCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGAGGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGGCGATTTACCCTCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATcagcagg  <  1:453668/250‑2 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           GCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGA  >  NC_000913/4629042‑4629515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑