Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,628,831 G→A intergenic (+284/+24) nadR → / ← ettA DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,628,8310GA84.4% 65.8 / 6.2 33intergenic (+284/+24)nadR/ettADNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base A (11/16);  total (12/21)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.34e-01

TTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCCACGAAATTGAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCA  >  NC_000913/4628590‑4629075
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
tttGATGAGTTGAAAACCTCTCTGGATGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCTCGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTTGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                                                                                                                                                                                              <  1:462134/250‑1 (MQ=255)
                                  gcgaaaatAAAAAAGGTTTTAAAAAACCCTGCAGGGGTGCCCCGCCACGAAATTGAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCtt                                                                                                                                                                                                             <  1:19146/243‑1 (MQ=255)
                                     aaataaaaCAAGGTT‑GAAAAAACCCGGCAGGGGTGCCCCGCCACGAAATTAAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACt                                                                                                                                                                                                        <  1:169341/246‑1 (MQ=255)
                                       aTTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTg                                                                                                                                                                                                      >  1:9455/1‑251 (MQ=255)
                                       aTTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGCCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTCCCTCGCAATACGCTTGCACTTg                                                                                                                                                                                                      >  1:471118/1‑251 (MQ=255)
                                           accAAGGTTTAAAAAACCC‑TGCCCGGGTGACCCGCAACGAAATTGCGGA‑TACTAGGGCTTTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATAc                                                                                                                                                                                                  <  1:283516/249‑1 (MQ=255)
                                           aaCAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATAc                                                                                                                                                                                                  >  1:82749/1‑251 (MQ=255)
                                                     gAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTc                                                                                                                                                                                        >  2:441187/1‑251 (MQ=255)
                                                        aaaaCC‑TGCAGGGGTGCCCCGCTAAGAATTTG‑GGACTACTAAGGCTGTGGTAGGCCGAAAAAGGCGTCCACGCCGTATCCGGCTTTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAg                                                                                                                                                                                     <  2:268221/251‑1 (MQ=255)
                                                                 cACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGACATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTg                                                                                                                                                                             >  1:189775/1‑251 (MQ=255)
                                                                                              tacTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTc                                                                                                                                                 >  2:60468/3‑251 (MQ=255)
                                                                                                      cTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATCCGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTc                                                                                                                                           >  2:116342/1‑249 (MQ=255)
                                                                                                        gttgtAGGCGGGAAAAGGCGTTCCCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCGTGTACTCTTCGTACTcag                                                                                                                                         <  1:127061/249‑3 (MQ=255)
                                                                                                               gcgtgaaaaggcgtTCACGCCTTATCCGGCATGCGGTGCTCAATGCCTGATGCGACGCTTACCTCGTCTGATCAGGCCTGTAGATTCACAACGTATTGAAAGTTCTTGGTGTGTAGGCCGGATAAGGTGTTCACGCCGCATCCGGCATGTTACGCATTACTTCGCAATACGCTTGTACTTGAGACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTAc                                                                                                                                <  1:455330/239‑1 (MQ=255)
                                                                                                                           gtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACt                                                                                                                    <  1:493422/251‑1 (MQ=255)
                                                                                                                                  ccgtaTCCGGCTTTCGGTGCTCAATGCCTGAGGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACTTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACttt                                                                                                             <  2:485447/247‑1 (MQ=255)
                                                                                                                                   cgcATCCGGCATTCGGTGCTTAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACttt                                                                                                             >  1:135254/1‑250 (MQ=255)
                                                                                                                                        ccGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTGGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGGAAAGTTACCTTCGAAGAACTCAACTTTACCTTc                                                                                                       <  1:136761/251‑1 (MQ=255)
                                                                                                                                                  gTGCTCAATGCCTGATGCGACGTTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTaa                                                                                             <  1:201310/251‑1 (MQ=255)
                                                                                                                                                          tGCCTGATGCGACTCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAggat                                                                                     >  1:115034/1‑248 (MQ=255)
                                                                                                                                                                       gCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATa                                                                        <  1:376689/251‑1 (MQ=255)
                                                                                                                                                                                    tatcagtcatgtatattcaCAACGTATTAAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCACCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAAc                                                            <  1:374077/232‑1 (MQ=255)
                                                                                                                                                                                              ggagattcccAACGTATTGAACTTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCg                                                   <  2:83770/240‑1 (MQ=255)
                                                                                                                                                                                                agattcaCAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCg                                               <  1:116342/245‑1 (MQ=255)
                                                                                                                                                                                                     caCAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGAt                                           <  1:441187/249‑1 (MQ=255)
                                                                                                                                                                                                       cAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAAc                                        <  2:135254/251‑1 (MQ=255)
                                                                                                                                                                                                         aCGTACTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTGTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCa                                      <  1:94078/251‑1 (MQ=255)
                                                                                                                                                                                                                         tggtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGa                       <  2:387235/247‑1 (MQ=255)
                                                                                                                                                                                                                           gtttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACt                    >  1:168984/2‑251 (MQ=255)
                                                                                                                                                                                                                                     cGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGc          >  1:225078/1‑251 (MQ=255)
                                                                                                                                                                                                                                            ggCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTcc   <  1:352352/251‑1 (MQ=255)
                                                                                                                                                                                                                                            ggCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTc    >  2:490259/1‑250 (MQ=255)
                                                                                                                                                                                                                                             gCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCa  <  1:339120/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    
TTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCCACGAAATTGAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCA  >  NC_000913/4628590‑4629075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑