Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,850	A→G	Y370Y (TAT→TAC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,850	0	A	G	97.0%	104.9 / ‑4.4	33	coding (1110/1431 nt)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (14/18);  minor base . (0/1);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCT  >  NC_000913/6606‑7081                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         ccGGGCTCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATg                                                                                                                                                                                                                                    <  1:418042/251‑1 (MQ=255)     gcgcACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑Gt                                                                                                                                                                                                                               <  1:349070/251‑1 (MQ=255)             cGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACg                                                                                                                                                                                                                       <  1:393761/251‑1 (MQ=255)                     cGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCCAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GATGGCAACGCTGGAGCt                                                                                                                                                                                                               >  1:182688/1‑251 (MQ=255)                            gTAGATAATCTCTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCAAGGCAGGCCATGATGATATCTGCCAGTTGCCACACCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCa                                                                                                                                                                                                        <  1:115300/251‑1 (MQ=255)                                      acgggcaagggtacaacccACAGGCGAGAGCAGTAAAATGGCGTTCAAATGGGTAATGGCCATGCAGACCATTATGAGAGCTGCCAGTGGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGAACCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑ATGGGCAACGATGGAGCTGAAGGCAAACAGAATaa                                                                                                                                                                                              <  2:34701/234‑1 (MQ=255)                                          gCAAGGGTATGACCCACAGGCGAGAGCAGGAAAATGGCGGTCAACTGGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTGGCCACATCAGCGGAAGACTTAGCAAGTTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGTTTTAGGGTTGTTCAGGCGGAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGATCTGAAGGCAAACAGAATAAcccc                                                                                                                                                                                          <  2:47108/251‑3 (MQ=255)                                          gCAAGGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcac                                                                                                                                                                                          <  2:300264/251‑1 (MQ=255)                                           cAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACcacg                                                                                                                                                                                         <  1:421674/251‑2 (MQ=255)                                               ggTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAg                                                                                                                                                                                                                 <  1:154632/223‑1 (MQ=255)                                                gtagtaaCCACAGGCGAGAGCATTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGt                                                                                                                                                                                    <  2:122347/246‑1 (MQ=255)                                                          caGGCGAGAGCAGGAAAATGGCGGGCAAATTGGGAATAGCCAGGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGACCGCTTTAGGGTTGTTCAGGCGGAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCa                                                                                                                                                                          <  2:278307/251‑1 (MQ=255)                                                                   gCAGTAAAATGGCGGTCAAATTGGTAATAGCCCTGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGGAGGGGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GGTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACGAACTCAGcccccc                                                                                                                                                                   >  2:183454/1‑249 (MQ=255)                                                                     aGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGTGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCaa                                                                                                                                                                                                       <  1:70702/211‑1 (MQ=255)                                                                        aaaaTGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAAcc                                                                                                                                                            <  1:218163/251‑1 (MQ=255)                                                                             ggtggTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTACATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACACCCGGAACCCAtt                                                                                                                                                        >  2:153228/4‑250 (MQ=255)                                                                                        gggTAATAGCCAGGCAGGCCAGTATGATATCTGCCAGTTGCCACAGCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACTATGGAGCTGAAGGCAAACAGAATAACCACGATGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATc                                                                                                                                            <  2:182688/250‑1 (MQ=255)                                                                                          gTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGc                                                                                                                                          >  1:377286/1‑251 (MQ=255)                                                                                           tAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGcc                                                                                                                                         >  1:60518/1‑251 (MQ=255)                                                                                               aGCCATGCAGGACATAATGATATCTGCCAGTTGCCACATCAGCGGAAGTCATAGCAAGGTGACGCCGATGGACGTTGCGAAGGTGCAGATCCGCAAACCCCAGATCGCTTTAGGGTTGTTCAGGCGTAGAAAGAAGAGAATGTTTTCCGCGTAAATGTT‑GCTGGCAACGATGGAGCTGAAGCCATACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGAAACACATTAACACCCGCAtagacctcc                                                                                                                                     >  1:455527/1‑243 (MQ=255)                                                                                                         gCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTg                                                                                                                           <  1:269406/251‑1 (MQ=255)                                                                                                               aTGATATCTGCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACCCCCGCATCGCCTTCTGGATAAGCTGAATAc                                                                                                                      <  1:258388/250‑1 (MQ=255)                                                                                                                        gCCAGTTGCCACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCgg                                                                                                            >  2:190732/1‑251 (MQ=255)                                                                                                                                ccACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCACACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGACTTCTGGATAAGCTTAATACCTTCCAGCGGCATGTAgg                                                                                                    >  1:114751/1‑251 (MQ=255)                                                                                                                                     tCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCCTGTAGGTtgtg                                                                                               >  2:388151/1‑251 (MQ=255)                                                                                                                                       agaggaagaattAGCAAGGGGCCGCCGATGACCGTTGCGAAGGTGAAGATCCGCAAACACCAGAGCGCTTTAGGGTGGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAAGAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTGCCAGCGGCATGTAGGTTGTGcc                                                                                             <  2:207718/241‑1 (MQ=255)                                                                                                                                                         gTGCCGCCGATGACCGTTGCGAAGGGGCAGATCCGCACACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGAAatat                                                                           >  2:66731/1‑251 (MQ=255)                                                                                                                                                                                    cAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGtt                                                                                                  <  1:333157/201‑1 (MQ=255)                                                                                                                                                                                          cgaaaaCACCAGTTCGTTTTAGGGTTGTGCAGAGGAAAAAAGAGAAGTTTGTTTGCGGC‑GAAAAGTATGTTGGCAACGAGGGAGCGGAAGGAAAACAGAATACCCACGAGGGAAACGACCCCAGCCCCCCAGGAACCCATAAACCCCCGCATCGCCTTCGGAAAAAGCTGAATCCCTCCCAGGGGCATGTAGTTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGgt                                          <  1:183454/248‑1 (MQ=255)                                                                                                                                                                                                    aGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaa                                 >  1:188676/1‑250 (MQ=255)                                                                                                                                                                                                     gATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCGTAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGAAGGTTGTGCCGTTCCCCGCCAGTAATATCAACAAGGCGCTTGCCGAAAAGGTGACCAGGGTGTCGTTaaaa                                >  2:241154/1‑250 (MQ=255)                                                                                                                                                                                                                        aGGCGTAAATAGCAGAGATTGTTTTCGGCGTAATTGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAACCTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTTCCGTAACGATGACCAGGGTGTCGATCAAAATGCCAATCATCTggccac             >  2:34828/1‑246 (MQ=255)                                                                                                                                                                                                                                  agaagaGATTGTTTTCGGCGTAACTGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACGAGGGTAACAAACTCAGCACCCCAGGAACCCATTAACACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGGGTCGATAAAAATGCCCATAATCTGGACACTCCCTTGCGCt  >  2:454718/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         CCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCCATTATGATATCTGCCAGTTGCCACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTCAGGCGTAAAAAGAAGAGATTGTTTTCGGCATAAATGTA‑GTTGGCAACGATGGAGCTGAAGGCAAACAGAATAACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGCGCT  >  NC_000913/6606‑7081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑