Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,668	C→T	intergenic (+121/+187)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,668	0	C	T	94.1%	92.6 / ‑3.5	35	intergenic (+121/+187)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (18/14);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 2.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCCACGAAATTGAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTG  >  NC_000913/4628420‑4628904                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              aGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGCGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGCAAAAACC‑TGCACGGGTGGCCCGCTACg                                                                                                                                                                                                                                            >  2:339120/1‑251 (MQ=255)    aCTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGaaa                                                                                                                                                                                                                                         <  1:295243/251‑1 (MQ=255)               gagaTGCTGGAAGAGAAAAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑tact                                                                                                                                                                                                                             <  2:257814/251‑4 (MQ=255)                  aTGCTGGAAGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTa                                                                                                                                                                                                                            >  1:34424/1‑249 (MQ=255)                         aaGAGAACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCtgttg                                                                                                                                                                                                                   >  1:1834/1‑251 (MQ=255)                                                     tGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCAcgccgtat                                                                                                                                                                                       >  1:497523/1‑248 (MQ=255)                                                                 ttatgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATACCCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAg                                                                                                                                                                                                                          >  2:213344/5‑204 (MQ=255)                                                                 ttatgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAg                                                                                                                                                                                                                          <  1:213344/200‑1 (MQ=255)                                                                                ccTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATg                                                                                                                                                            >  1:47133/1‑251 (MQ=255)                                                                                  tgcgctgcgTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCg                                                                                                                                                          >  2:104251/1‑251 (MQ=255)                                                                                                                    ggtgCAGATATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATTAGTTGAAATCCTCTCTGGAAGCGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGGGTCCCGCTACGAAATTGCGGA‑TACTAATGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCtggagattca                                                                                                                        <  1:485711/248‑10 (MQ=255)                                                                                                                           agataccctcgataaaaCTGCTCAAAGGCGGGGTATAAAAGAAGTTTTTTAG‑TGAGTGGAAACCCTCTCTGGAAGAGGCTGCCGAGATTAAACAAGGTTGGAAAAAACC‑GGCACGGGTGACCCGCTACTAAATTGCGGA‑TACGAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTa                                                                                                                 <  2:269064/237‑1 (MQ=255)                                                                                                                           agaTAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCtggagattcacaacgt                                                                                                                  >  1:94665/1‑235 (MQ=255)                                                                                                                             aTAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGAAAGAGCCTGTCGATATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTAtt                                                                                                               <  1:294025/251‑1 (MQ=255)                                                                                                                                      atgaAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCg                                                                                                                                                                                                            <  1:408308/149‑1 (MQ=255)                                                                                                                                      atgaAACGGCTCAAAGGCGAGGGATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGCAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCg                                                                                                                                                                                                            >  2:408308/1‑149 (MQ=255)                                                                                                                                         aaaCTGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GAGGGGATGAAACCCTCTCTGGAAGAGGCTGTCGAAATTAAACAAGGTTTGAAAAACCC‑TGCAGGGGTGACCCGCTCCGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttgg                                                                                                   <  2:190454/251‑7 (MQ=255)                                                                                                                                              gCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAACCC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGcc                                                                                                                                                                 <  2:427739/184‑1 (MQ=255)                                                                                                                                              gCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGcc                                                                                                                                                                 >  1:427739/1‑184 (MQ=255)                                                                                                                                                             ataAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAACCC‑TGCACGGGGGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCtgga                                                                                                                              <  2:325104/204‑4 (MQ=255)                                                                                                                                                             ataAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCtgga                                                                                                                              >  1:325104/1‑201 (MQ=255)                                                                                                                                                             ataAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCg                                                                               >  1:387235/1‑251 (MQ=255)                                                                                                                                                             ataAAATAAGTTTTTT‑GAGGAGTTGAAACCCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGc                                                                                <  1:104251/250‑1 (MQ=255)                                                                                                                                                                    aaagttttgtGATGAGTGTAAAACCTCTTGGGAAGAGGCTGTCGAGATAAAAAAAGGTTTGAAAAAACC‑TGCACGGGGGACCCGCTACGAAATTGCGGA‑TACGAGGGCGGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTcacg                                                                         <  2:120610/247‑4 (MQ=255)                                                                                                                                                                           tttGATGAGTTGAAAACCTCTCTGGATGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCTCGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTTGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                                   <  1:462134/250‑1 (MQ=255)                                                                                                                                                                                                          cTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTcac                                                                          >  1:310207/1‑210 (MQ=255)                                                                                                                                                                                                             gcgaaaatAAAAAAGGTTTTAAAAAACCCTGCAGGGGTGCCCCGCCACGAAATTGAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCtt                                  <  1:19146/243‑1 (MQ=255)                                                                                                                                                                                                                aaataaaaCAAGGTT‑GAAAAAACCCGGCAGGGGTGCCCCGCCACGAAATTAAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACt                             <  1:169341/246‑1 (MQ=255)                                                                                                                                                                                                                  aTTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTg                           >  1:9455/1‑251 (MQ=255)                                                                                                                                                                                                                  aTTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGCCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTCCCTCGCAATACGCTTGCACTTg                           >  1:471118/1‑251 (MQ=255)                                                                                                                                                                                                                      accAAGGTTTAAAAAACCC‑TGCCCGGGTGACCCGCAACGAAATTGCGGA‑TACTAGGGCTTTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATAc                       <  1:283516/249‑1 (MQ=255)                                                                                                                                                                                                                      aaCAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATAc                       >  1:82749/1‑251 (MQ=255)                                                                                                                                                                                                                                gAAAAAACC‑TGCACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTc             >  2:441187/1‑251 (MQ=255)                                                                                                                                                                                                                                   aaaaCC‑TGCAGGGGTGCCCCGCTAAGAATTTG‑GGACTACTAAGGCTGTGGTAGGCCGAAAAAGGCGTCCACGCCGTATCCGGCTTTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAg          <  2:268221/251‑1 (MQ=255)                                                                                                                                                                                                                                            cACGGGTGACCCGCTACGAAATTGCGGA‑TACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGACATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTg  >  1:189775/1‑251 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              AGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACC‑TGCACGGGTGACCCGCCACGAAATTGAGGA‑TGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTG  >  NC_000913/4628420‑4628904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑