Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,574	A→G	G462G (GGT→GGC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,574	0	A	G	97.6%	117.8 / NA	42	G462G (GGT→GGC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (18/23);  minor base T (0/1);  total (18/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

GTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTC  >  NC_000913/6339‑6820                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                         gTTTCCGCGCTTCATGGATCAACTGGTGGGAATTGTCTAACAGGTCCGGCAGCGTATAGCGCGTTGTGGTCAACGGGCTTTGGTAATCAAGCTGTTGCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTGTAGCAAAAAAAGAGAATGAGTTGGTCGATAGTTGTGATTACGCCGGCGAAACATCATCCCACGCGTCCGGATAAAGCTGGCGGCCGATATCCGGATaa                                                                                                                                                                                                                                           <  1:63266/251‑1 (MQ=255)        cgcttCATGGCTCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGCTAACGCaacg                                                                                                                                                                                                                                    >  2:218163/5‑249 (MQ=255)        cgcttCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCaacg                                                                                                                                                                                                                                    >  1:185012/5‑249 (MQ=255)                                aTTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCg                                                                                                                                                                                                             >  2:173077/1‑250 (MQ=255)                                  tGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGt                                                                                                                                                                                                          >  1:214428/1‑251 (MQ=255)                                   gTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGAGAACGCATCGGATCAAACACCGGGCGCACGCCGAGtt                                                                                                                                                                                                         <  2:248266/251‑1 (MQ=255)                                           gCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTgg                                                                                                                                                                                                 >  1:23262/1‑251 (MQ=255)                                                         taGCGCGTGGAGGTCAACGGGCTTTGGAAATAAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCAcc                                                                                                                                                                                   >  1:352460/1‑250 (MQ=255)                                                                    gttcagcGGGCTTTGGTAATCAATCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAACAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTAt                                                                                                                                                                        <  2:427860/245‑1 (MQ=255)                                                                                  ggTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCgag                                                                                                                                                          <  1:448237/251‑1 (MQ=255)                                                                                    tAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCgag                                                                                                                                                          >  1:475187/1‑249 (MQ=255)                                                                                     aaTCAAGCGTTTTCGCAGGTGAAATAAGAATCAGGATATCCAGTCCTTGCAAGAAATTTATGCCGACTTTAGCAAAAAAAGAGAGTGATTTGATCGATAGTTGTGATTACTCCTGCGAAACATCACCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGagag                                                                                                                                                        <  2:499574/250‑1 (MQ=255)                                                                                        cAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAg                                                                                                                                                     <  1:173077/250‑1 (MQ=255)                                                                                        acaGCGTTTTCGCAGGTGAAATAAGAATCAGCATAAACAGGCCTGTCAGGAAATTTATGCCGAATTTAGCAAAAAAAGAGAATGAGGTGATCGATAGTTGGGATTCCTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGTCCGATATCCGGTTAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCTAGAGCac                                                                                                                                                     <  2:468884/248‑2 (MQ=255)                                                                                             ggtttCGCAGGTGAAATAAGAAGCAGCATATCCAGTTCTTTCAGGAAAATTATGCCGACTTGAGCAAAAAAAGGAAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCAGCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGGTAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAAt                                                                                                                                               <  2:32944/249‑1 (MQ=255)                                                                                             gTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAAt                                                                                                                                               <  2:123300/251‑1 (MQ=255)                                                                                               tttCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATg                                                                                                                                              <  1:71287/250‑1 (MQ=255)                                                                                                       gTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCgag                                                                                                                                                          >  1:258352/1‑230 (MQ=255)                                                                                                       gTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCaaa                                                                                                                                     <  2:475187/251‑1 (MQ=255)                                                                                                       gTGAAATAAGAATCAGCATATCCAGGCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCgag                                                                                                                                                          <  2:258352/230‑1 (MQ=255)                                                                                                             tAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGACCCACAGGCGAGAGCAGTAAAATGGCGGTCCAATTGGt                                                                                                                                >  1:148029/1‑250 (MQ=255)                                                                                                                gaaacAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTGGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATa                                                                                                                            <  2:505208/247‑1 (MQ=255)                                                                                                                          atCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCTGCCGATATCCGGATAACGCAACTGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAg                                                                                                                   <  1:17930/250‑1 (MQ=255)                                                                                                                            ccAGTCCTTGCAGGAAATGTATGCCGACTTTAGCAAAAAAAGAGAAGGAGGTGAAAGAGAGTTGGGATTACTCCTGCGAAACAACATCCCACGCGTCCGGAGAAAGATGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGACGAGGGTACGCTGGAGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATGGGTAATAGCCATGCAGGcc                                                                                                                <  2:450195/251‑1 (MQ=255)                                                                                                                                 tcTTGCAGTAAATTTATGCCGACTTTAGCAAAAAAAGAGAATTAGTGGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTa                                                                                                           <  1:351118/249‑1 (MQ=255)                                                                                                                                        ggAAATTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCt                                                                                                   <  1:105949/251‑1 (MQ=255)                                                                                                                                            aTTTATGCCGACTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGTATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCa                                                                                               <  1:146452/251‑1 (MQ=255)                                                                                                                                                      aCTTTAGCAAAAAAAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTaaa                                                                                                                                                 >  1:258471/1‑192 (MQ=255)                                                                                                                                                          tAGCAAAAAAAGAGAATGAGTTGACCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAg                                                                                 <  1:12746/250‑1 (MQ=255)                                                                                                                                                           aGCAAAAAAAGAGAATGAGTTGATCTATAGTTGTGATTACTCCTGCGAACCATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGc                                                                                <  2:185012/250‑1 (MQ=255)                                                                                                                                                                aaaaAGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAga                                                                          <  1:15856/251‑2 (MQ=255)                                                                                                                                                                       gaatgagTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGACTTAGCa                                                                   <  2:168567/251‑1 (MQ=255)                                                                                                                                                                                 aTCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAAACTTAGCAAGGGgccgcc                                                         >  1:244801/1‑251 (MQ=255)                                                                                                                                                                                        gTTGTGATTACTCCTGCGAAACATCATCCCACGCGCCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGACTTAGCAAGGTGCCGGCGATGAc                                                   <  1:165391/250‑1 (MQ=255)                                                                                                                                                                                         ttGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAACCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCg                                                 >  1:278307/1‑251 (MQ=255)                                                                                                                                                                                                          aaaCATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGA‑CCCATCAGCGGAAGACTTAGCAAGGGGCCGCCGATGACCGTTGCGagggggc                                     >  1:274271/1‑240 (MQ=255)                                                                                                                                                                                                            aCATCATCCCACGCGTCCGGAGAAAGCTGGCGGCCGCTATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCCAATTGGTAATAGCCATGCCGGCCCATTA‑GTTATTTGCCCGTTGCACACACCA‑CGGAAAACTTACCAAGGTGCACCCGATGACCGTTGCGaaaggggagaaac                               >  2:313547/1‑239 (MQ=255)                                                                                                                                                                                                                     cACGCGTCCGGAGAAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGACAAATTGGTAATAGCCAAGCCGGCC‑ATTTTGATATCTGCCAGTTGC‑CACATCAGCGGGAGAATTAGAAAGGGGCCCCCGGTGACCGTTGccgaggggccggaccgccaaaaac                      >  2:421674/1‑227 (MQ=255)                                                                                                                                                                                                                        gcgTCCGGAGAAAGCTGGCTGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCCAATTGGAAATACCCATTCATGTC‑CTTATGATATCTGCCAGTTGA‑CACATCAGCGGAAGACTTAGCGAGGTGCCGCCGATGACCGTTGCGCAGGTGACGATCCGCAAACAcc                      >  1:115176/1‑247 (MQ=255)                                                                                                                                                                                                                               gagaAAGCTGGCGGCCGATATCCGGATAACGAAACGGATCAAACACCGGGCGCACGCCTAGGTTACGCTGGCGGAGATAATCACTGGCAATGGTAGGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGACTGAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTa           <  1:496934/251‑1 (MQ=255)                                                                                                                                                                                                                                 gaAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGCGGGCAGTAAAATGGCGGTCAAATTGGTAATAGCCCTGCCAGCC‑ATTATGATATCGGCCGATTGC‑CACCTCAACGGAAAACTTCGCAAGCAGCCGCCGAAGACCGTTGCGACGGGGCAGATCCGAAAACACCAGATCGccttggg         >  1:207718/1‑245 (MQ=255)                                                                                                                                                                                                                                 gaAAGCTGGCGGCCGATATCCGGATAACGCAACGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCCAACACCAGATCGCTTTAgg         >  1:146504/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggcggcCGATATCCGGATAACGCAACGGATCAAACACCGGGCCCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGACTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTc  <  1:427365/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                         GTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATGCCGACTTTAGCAAAAAATGAGAATGAGTTGATCGATAGTTGTGATTACTCCTGCGAAACATCATCCCACGCGTCCGGAGAAAGCTGGCGACCGATATCCGGATAACGCAATGGATCAAACACCGGGCGCACGCCGAGTTTACGCTGGCGTAGATAATCACTGGCAATGGTATGAACCACAGGCGAGAGCAGTAAAATGGCGGTCAAATTGGTAATAGCCATGCAGGCC‑ATTATGATATCTGCCAGTTGC‑CACATCAGCGGAAGGCTTAGCAAGGTGCCGCCGATGACCGTTGCGAAGGTGCAGATCCGCAAACACCAGATCGCTTTAGGGTTGTTC  >  NC_000913/6339‑6820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑