Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,627,653 C→T A113A (GCC→GCT)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,6530CT97.5% 113.2 / ‑4.1 40A113A (GCC→GCT) nadRDNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (12/27);  total (12/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

TTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCA‑GCCGACCGTG‑CCGGATCGTCTGCGTTGGT‑TATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCG  >  NC_000913/4627408‑4627891
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
ttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGt                                                                                                                                                                                                                                               <  1:142371/250‑1 (MQ=255)
  aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCgc                                                                                                                                                                                                                                            >  1:254648/1‑251 (MQ=255)
               aaTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCGGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGATCTACCCGCGGCATACCGGACATAGCTACCTTATCCAGCGCGCCTGTAGCCAGGGTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCAACCGt                                                                                                                                                                                                                              <  2:98429/251‑1 (MQ=255)
                  gcccaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGGTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCCCCGTG‑cc                                                                                                                                                                                                                          <  2:450667/247‑1 (MQ=255)
                   ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCg                                                                                                                                                                                                                         <  1:25249/251‑1 (MQ=255)
                   ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGGG‑CCg                                                                                                                                                                                                                         >  2:12750/1‑251 (MQ=255)
                        aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTATAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCA‑GCCGACCGTG‑CCGGATCg                                                                                                                                                                                                                    <  1:101090/251‑1 (MQ=255)
                              aGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCCGGTTGACGCACTGCATATCATTCTGGGGTTTGACGAAACCCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCATCA‑GCC‑ACCCTGGCCGGATCGTcgggg                                                                                                                                                                                                              >  2:442423/1‑247 (MQ=255)
                                 cccAGCGCGCAAAAGCGGGAGGCGTTGCGCCGGTTTTTGGGGCTTGAGTTTCCCCGGACGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCgctg                                                                                                                                                                                                           <  2:136758/251‑4 (MQ=255)
                                         gcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttac                                                                                                                                                                                                  <  1:215229/251‑3 (MQ=255)
                                          cAAAAGCTGGAGGCGTTGCACCGTTTGTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttaca                                                                                                                                                                                                 <  2:6650/251‑4 (MQ=255)
                                             aaGCTGGAGCCGTTGCACCGCTTTTTGGGGCTTGAGTTTCCCCGGGAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTTCCCGCTGCATACCGGTCATATCTACCTTATCCAGCGCGCCTGTAGCCTGTTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCTCGATCGCGCGTTGTTTGAAGACATCGCTATGTCGCAGCA‑TCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAAc                                                                                                                                                                                              <  1:486055/251‑1 (MQ=255)
                                                cTGGCGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCA‑CACGCCCACCGTG‑CCGGATCGGCTGCGCTGGT‑TATTACAAACttt                                                                                                                                                                                           >  2:92958/1‑251 (MQ=255)
                                                     ggCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAat                                                                                                                                                                                      >  1:502069/1‑251 (MQ=255)
                                                        gTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGG‑TATTACAAACTTTTAAATATc                                                                                                                                                                                   >  1:475579/1‑251 (MQ=255)
                                                           gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGa                                                                                                                                                                                <  2:90311/251‑1 (MQ=255)
                                                                gTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTGTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAat                                                                                                                                                                           <  1:20338/251‑1 (MQ=255)
                                                                       ggggTTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCTCCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCa                                                                                                                                                                    <  2:94924/251‑1 (MQ=255)
                                                                        gggCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCATCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCAt                                                                                                                                                                   <  1:244186/251‑1 (MQ=255)
                                                                         ggCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATAGCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCAtt                                                                                                                                                                  <  2:254648/251‑1 (MQ=255)
                                                                          gCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTc                                                                                                                                                                 <  1:235003/251‑1 (MQ=255)
                                                                              gAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATTTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATAGCATTATGGGTTTTGACGATTCCCGCGATCGCTCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATg                                                                                                                                                              <  1:464361/250‑1 (MQ=255)
                                                                                         gCAGAAGAAAACCATTGGTGTGGTATTCGGTAATTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTATCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAag                                                                                                                                                  <  1:506993/251‑1 (MQ=255)
                                                                                                 aaaccattggTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGCCATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTAATGCTTTCAACGAAGAGGGCATg                                                                                                                                          >  2:354888/9‑251 (MQ=255)
                                                                                                    ccattggTGTGGTATTCTGTAAGTTCTACCCGCTTCATCCCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATAGCAGTATGTGTTTTGACGATCCCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGACCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATGCGCATTCATGCTTTCAACGAAGAGGGCATGGAg                                                                                                                                       <  1:90778/246‑1 (MQ=255)
                                                                                                               gTATTCGGTAAGTTCTACCCGTTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCa                                                                                                                            <  1:430250/251‑1 (MQ=255)
                                                                                                                               aCCCGCTGCATACCGGACATATCAACCTTAACCAGCGCGCCTGTACACAGGCTGACGACCTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGACCACAGCGCTATGTCGCAGCC‑GCCCACCGTG‑CCGGATCGTCTGCGGTGGTCTAT‑AAAAACTTTTAAAAATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGAATCCGCAGGGCTGGCGTGTGTgg                                                                                                            >  2:68995/1‑251 (MQ=255)
                                                                                                                                cccgcTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTAGGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGa                                                                                                           <  2:356291/247‑1 (MQ=255)
                                                                                                                                           ccGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGGTTTGACGATACCCGCGATCGCGCGTTGGTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc                                                                                                <  2:414271/251‑1 (MQ=255)
                                                                                                                                                       aCCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTa                                                                                      <  1:192139/249‑1 (MQ=255)
                                                                                                                                                         cTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGGATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGc                                                                                  >  1:23735/1‑251 (MQ=255)
                                                                                                                                                          ttATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttaca                                                                                                                                                                                                 >  2:455801/1‑136 (MQ=255)
                                                                                                                                                          ttATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGAGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttaca                                                                                                                                                                                                 <  1:455801/139‑4 (MQ=255)
                                                                                                                                                                aGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGATTTGACGATACCAGCGATAGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑ACGGCTCGTCTGCGCTGGT‑TATTACAACCTTTTAAATATCAGAACAATATTCGCATTCATGCTTTCAACGAAGAGGGACTGGAGCCGTATCCGCACGGCTGGGATTGGTGGGACAACGGCATCAAGAATTTAATGGCTGaaccg                                                                           >  2:158536/1‑248 (MQ=255)
                                                                                                                                                                  cgcgcCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaaaggg                                                                         <  1:279793/251‑4 (MQ=255)
                                                                                                                                                                    cgcCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGa                                                                       <  1:225938/251‑1 (MQ=255)
                                                                                                                                                                         gTAGCCAGGTTGACGAACTGCATTTCATTATGTGTTTTGACGATTCCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAg                                                                  <  1:38958/251‑1 (MQ=255)
                                                                                                                                                                                                             ttGACGAGACCCTCGATCGCGCGTTGTACGAAGACAGCGATATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTGTGCGCTGGT‑TATTACAAACTTTTGAATATCAGAAAAATATTCGCATTCTTCCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtg                                                                                                                 <  1:300770/168‑1 (MQ=255)
                                                                                                                                                                                                                      cccgcgagcgcgcgTTGGTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACGTTTAAATATCAGAAAAATATTCGCATTCAGGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATTTGTGGATCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGtat                     <  2:475579/243‑1 (MQ=255)
                                                                                                                                                                                                                                         tgAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGCTTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCg  >  2:374045/2‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 
TTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCA‑GCCGACCGTG‑CCGGATCGTCTGCGTTGGT‑TATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCG  >  NC_000913/4627408‑4627891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑