Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,627,641 C→T F109F (TTC→TTT)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,627,6410CT92.9% 109.5 / ‑1.3 42F109F (TTC→TTT) nadRDNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base T (12/27);  total (12/30)
Fisher's exact test for biased strand distribution p-value = 5.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

ATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCA‑GCCGACCGTG‑CCGGATCGTCTGCGTTGGT‑TATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTAT  >  NC_000913/4627393‑4627872
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
aTGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCg                                                                                                                                                                                                                                           <  2:139696/250‑1 (MQ=255)
  gACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAg                                                                                                                                                                                                                                        <  1:273660/251‑1 (MQ=255)
         gggTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGAGCGTTGTTTGAAGACAgcgc                                                                                                                                                                                                                                 >  1:326613/1‑248 (MQ=255)
               ttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGt                                                                                                                                                                                                                            <  1:142371/250‑1 (MQ=255)
                 aaGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCgc                                                                                                                                                                                                                         >  1:254648/1‑251 (MQ=255)
                              aaTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCGGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGATCTACCCGCGGCATACCGGACATAGCTACCTTATCCAGCGCGCCTGTAGCCAGGGTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCAACCGt                                                                                                                                                                                                           <  2:98429/251‑1 (MQ=255)
                                 gcccaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGGTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCCCCGTG‑cc                                                                                                                                                                                                       <  2:450667/247‑1 (MQ=255)
                                  ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCg                                                                                                                                                                                                      <  1:25249/251‑1 (MQ=255)
                                  ccAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGGG‑CCg                                                                                                                                                                                                      >  2:12750/1‑251 (MQ=255)
                                       aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTATAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCA‑GCCGACCGTG‑CCGGATCg                                                                                                                                                                                                 <  1:101090/251‑1 (MQ=255)
                                             aGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCCGGTTGACGCACTGCATATCATTCTGGGGTTTGACGAAACCCGCGATCGCGCGGTGTTTGAAGACAGCGCTATGTCGCATCA‑GCC‑ACCCTGGCCGGATCGTcgggg                                                                                                                                                                                           >  2:442423/1‑247 (MQ=255)
                                                cccAGCGCGCAAAAGCGGGAGGCGTTGCGCCGGTTTTTGGGGCTTGAGTTTCCCCGGACGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCgctg                                                                                                                                                                                        <  2:136758/251‑4 (MQ=255)
                                                        gcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttac                                                                                                                                                                               <  1:215229/251‑3 (MQ=255)
                                                         cAAAAGCTGGAGGCGTTGCACCGTTTGTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttaca                                                                                                                                                                              <  2:6650/251‑4 (MQ=255)
                                                            aaGCTGGAGCCGTTGCACCGCTTTTTGGGGCTTGAGTTTCCCCGGGAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTTCCCGCTGCATACCGGTCATATCTACCTTATCCAGCGCGCCTGTAGCCTGTTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCTCGATCGCGCGTTGTTTGAAGACATCGCTATGTCGCAGCA‑TCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAAc                                                                                                                                                                           <  1:486055/251‑1 (MQ=255)
                                                               cTGGCGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCA‑CACGCCCACCGTG‑CCGGATCGGCTGCGCTGGT‑TATTACAAACttt                                                                                                                                                                        >  2:92958/1‑251 (MQ=255)
                                                                    ggCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAat                                                                                                                                                                   >  1:502069/1‑251 (MQ=255)
                                                                       gTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGG‑TATTACAAACTTTTAAATATc                                                                                                                                                                >  1:475579/1‑251 (MQ=255)
                                                                          gCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGa                                                                                                                                                             <  2:90311/251‑1 (MQ=255)
                                                                               gTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTGTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAat                                                                                                                                                        <  1:20338/251‑1 (MQ=255)
                                                                                      ggggTTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCTCCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCa                                                                                                                                                 <  2:94924/251‑1 (MQ=255)
                                                                                       gggCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCATCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCAt                                                                                                                                                <  1:244186/251‑1 (MQ=255)
                                                                                        ggCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATAGCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCAtt                                                                                                                                               <  2:254648/251‑1 (MQ=255)
                                                                                         gCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTc                                                                                                                                              <  1:235003/251‑1 (MQ=255)
                                                                                             gAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATTTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATAGCATTATGGGTTTTGACGATTCCCGCGATCGCTCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATg                                                                                                                                           <  1:464361/250‑1 (MQ=255)
                                                                                                        gCAGAAGAAAACCATTGGTGTGGTATTCGGTAATTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTATCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAag                                                                                                                               <  1:506993/251‑1 (MQ=255)
                                                                                                                aaaccattggTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGCCATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTAATGCTTTCAACGAAGAGGGCATg                                                                                                                       >  2:354888/9‑251 (MQ=255)
                                                                                                                   ccattggTGTGGTATTCTGTAAGTTCTACCCGCTTCATCCCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATAGCAGTATGTGTTTTGACGATCCCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGACCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATGCGCATTCATGCTTTCAACGAAGAGGGCATGGAg                                                                                                                    <  1:90778/246‑1 (MQ=255)
                                                                                                                              gTATTCGGTAAGTTCTACCCGTTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCa                                                                                                         <  1:430250/251‑1 (MQ=255)
                                                                                                                                              aCCCGCTGCATACCGGACATATCAACCTTAACCAGCGCGCCTGTACACAGGCTGACGACCTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGACCACAGCGCTATGTCGCAGCC‑GCCCACCGTG‑CCGGATCGTCTGCGGTGGTCTAT‑AAAAACTTTTAAAAATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGAATCCGCAGGGCTGGCGTGTGTgg                                                                                         >  2:68995/1‑251 (MQ=255)
                                                                                                                                               cccgcTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTAGGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGa                                                                                        <  2:356291/247‑1 (MQ=255)
                                                                                                                                                          ccGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGGTTTGACGATACCCGCGATCGCGCGTTGGTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATc                                                                             <  2:414271/251‑1 (MQ=255)
                                                                                                                                                                      aCCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTa                                                                   <  1:192139/249‑1 (MQ=255)
                                                                                                                                                                        cTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGGATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGc                                                               >  1:23735/1‑251 (MQ=255)
                                                                                                                                                                         ttATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttaca                                                                                                                                                                              >  2:455801/1‑136 (MQ=255)
                                                                                                                                                                         ttATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGAGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TAttaca                                                                                                                                                                              <  1:455801/139‑4 (MQ=255)
                                                                                                                                                                               aGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGATTTGACGATACCAGCGATAGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑ACGGCTCGTCTGCGCTGGT‑TATTACAACCTTTTAAATATCAGAACAATATTCGCATTCATGCTTTCAACGAAGAGGGACTGGAGCCGTATCCGCACGGCTGGGATTGGTGGGACAACGGCATCAAGAATTTAATGGCTGaaccg                                                        >  2:158536/1‑248 (MQ=255)
                                                                                                                                                                                 cgcgcCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGaaaaggg                                                      <  1:279793/251‑4 (MQ=255)
                                                                                                                                                                                   cgcCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGa                                                    <  1:225938/251‑1 (MQ=255)
                                                                                                                                                                                        gTAGCCAGGTTGACGAACTGCATTTCATTATGTGTTTTGACGATTCCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAg                                               <  1:38958/251‑1 (MQ=255)
                                                                                                                                                                                                                            ttGACGAGACCCTCGATCGCGCGTTGTACGAAGACAGCGATATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTGTGCGCTGGT‑TATTACAAACTTTTGAATATCAGAAAAATATTCGCATTCTTCCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGAtg                                                                                              <  1:300770/168‑1 (MQ=255)
                                                                                                                                                                                                                                     cccgcgagcgcgcgTTGGTTGAAGACAGCGCTATGTCGCAGCA‑GCCCACCGTG‑CCGGATCGTCTGCGCTGGT‑TATTACAAACGTTTAAATATCAGAAAAATATTCGCATTCAGGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATTTGTGGATCAACGGCATCAAAAAGTTTATGGCTGAAAAGGGGATTCAGCCGGACCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGtat  <  2:475579/243‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          
ATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCA‑GCCGACCGTG‑CCGGATCGTCTGCGTTGGT‑TATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGTATCCGCACGGCTGGGATGTGTGGAGCAACGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTAT  >  NC_000913/4627393‑4627872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑