Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,377	A→G	V21V (GTA→GTG)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,377	0	A	G	96.2%	156.6 / ‑2.6	52	V21V (GTA→GTG)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (27/23);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

CGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCG  >  NC_000913/4627134‑4627625                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         cGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATg                                                                                                                                                                                                                                                   <  1:325931/251‑1 (MQ=255)        cAGCGGTCAGGAACGAATTTCAGAAGCAGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCgg                                                                                                                                                                                                                                            <  2:348727/251‑1 (MQ=255)           cGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTAt                                                                                                                                                                                                                                         <  1:438479/251‑1 (MQ=255)               cAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGAc                                                                                                                                                                                                                                      <  1:1891/250‑1 (MQ=255)                    aCGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAgg                                                                                                                                                                                                                                <  1:490622/251‑1 (MQ=255)                           tcagAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTAttt                                                                                                                                                                                                                          <  1:424515/247‑1 (MQ=255)                             agAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGCATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTa                                                                                                                                                                                                                         >  1:42100/2‑249 (MQ=255)                             agAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAg                                                                                                                                                                                                                       <  2:235353/250‑1 (MQ=255)                              gAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGc                                                                                                                                                                                                                      >  1:384237/1‑251 (MQ=255)                                   ggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGtt                                                                                                                                                                                                                 >  1:103859/1‑251 (MQ=255)                                     cgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGcc                                                                                                                                                                                                                     <  2:360337/245‑1 (MQ=255)                                     cgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGcc                                                                                                                                                                                                                     >  1:360337/1‑245 (MQ=255)                                          cactgTTTTCGTCGGGAGATTGTTCCGGAGGCTCACGTACCGAAAACAGCGCCGGAAGTGATGCAGAATTGTGGCGTTAAAAAACTCTCCGACGCTCGTAGCGTGTTCGACGACTTATAAAGAGGAATCCGGAGGGAGATATGTCGTCATTTGATTACCGGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGATATTTAAGCCAGTTCCTGAAt                                                                                                                                                                                                          <  2:109062/247‑1 (MQ=255)                                           ccgggtttCGCCGGGGGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGTTATTTAAGCCAGTTACTGAATg                                                                                                                                                                                                         <  2:277760/246‑1 (MQ=255)                                           acGGTTTTCGCTGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGAGTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATg                                                                                                                                                                                                         <  2:103859/251‑1 (MQ=255)                                                  tCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGTAAGGGATGCAGATTTTTGGCGGTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCaaaa                                                                                                                                                                                                   <  2:504742/250‑1 (MQ=255)                                                  tCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAAt                                                                                                                                                                                                  >  1:90311/1‑251 (MQ=255)                                                   cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGCCCAAAGGGTATTTAAGCCAGTTACTGAATGCCACAATc                                                                                                                                                                                                 >  1:263884/1‑251 (MQ=255)                                                          ggATTGTTCCGGCGGCTAACGTACAGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACGGAATGCCAAAATCAAAAGcc                                                                                                                                                                                          <  2:395397/250‑1 (MQ=255)                                                          cGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACGCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGcc                                                                                                                                                                                          <  1:280862/251‑1 (MQ=255)                                                                       ggcTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCaaa                                                                                                                                                                              >  1:450667/1‑250 (MQ=255)                                                                         cTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCCAAATCAAAAGCCCAAGCGCGCAAAAGc                                                                                                                                                                           >  1:288554/1‑251 (MQ=255)                                                                             cgaaacGAAAAAAGCGCCGGAAGGGATCCAGTATTTTGACGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTGGACGACTTATAATGAGGAATACGGAGGTAGATATGTCGGCATTAGATTACCTGAAAACGGCCATCAAGCAACATGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGGATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCTCAGCGCGCAAAAGCTGGa                                                                                                                                                                       <  2:40284/246‑1 (MQ=255)                                                                               taccGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGGATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAgg                                                                                                                                                                     >  2:154487/3‑251 (MQ=255)                                                                                   gAAAAAAGCGCCGTAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGGCGGCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGtt                                                                                                                                                                 <  2:384237/251‑1 (MQ=255)                                                                                   gAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGCCTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAAAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGtt                                                                                                                                                                 >  1:17014/1‑251 (MQ=255)                                                                                                 aaGGGATGCAGATTTTTGGCGTTAAAAAACGCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGGCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTgg                                                                                                                                                   <  2:304876/251‑1 (MQ=255)                                                                                                  aGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTggg                                                                                                                                                  >  1:356291/1‑251 (MQ=255)                                                                                                          aGATTTTTGGCGTTAAACAACTCTCCGCAGCGCTAAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTCCCTGAAAACTGCCCTCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGCTGCCAGCGGTATGAGCAAAGGGTCTTTAAGCAAGTTACTGACTGCCAACATAAAAAGCCCCAGCGCGCAACCGCTGGAGGAGTTGCACCGTTTTTTGGGGCTTGAGt                                                                                                                                          >  2:38958/1‑251 (MQ=255)                                                                                                                  ggCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCgg                                                                                                                                  >  2:379004/1‑251 (MQ=255)                                                                                                                      ttAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGCGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGAATTTACGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGc                                                                                                                                 >  2:192139/1‑248 (MQ=255)                                                                                                                              ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAAcc                                                                                                                      >  1:94924/1‑250 (MQ=255)                                                                                                                                         gcgtAGCGTGTGCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCTCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGgtgtggt                                                                                                           <  1:186178/248‑4 (MQ=255)                                                                                                                                         gcTTAGCGTGTTCGACGCCTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGgtggggt                                                                                                           >  2:437141/1‑247 (MQ=255)                                                                                                                                            tAGCGTGGTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCATAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTAt                                                                                                         <  2:265195/250‑1 (MQ=255)                                                                                                                                                 tgtTCGACGACTTATAAGGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGAAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACAATTGGTGTGGTATTCGGt                                                                                                    >  2:25249/1‑250 (MQ=255)                                                                                                                                                     cgacgaCTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGtt                                                                                               >  2:273660/1‑251 (MQ=255)                                                                                                                                                         gacTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGTGTGGTATTCGGTAAGTTCTAc                                                                                           >  1:328638/1‑251 (MQ=255)                                                                                                                                                          acTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcc                                                                                          >  2:235003/1‑251 (MQ=255)                                                                                                                                                                              agggagATATGTAGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACatatt                                                                      >  1:407811/1‑250 (MQ=255)                                                                                                                                                                                  agaTATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGACTTGCGTTTCCCCGGCAGAAGAAAACCATTGGGGTGGTATTCGGTCATTTTTACCCGCTGCAAACCGGACATATCTAcc                                                                  >  2:279793/1‑251 (MQ=255)                                                                                                                                                                                        gtcgtcATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTgggg                                                                                                                                                 <  2:277377/166‑1 (MQ=255)                                                                                                                                                                                        gtcgtcATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTgggg                                                                                                                                                 >  1:277377/1‑166 (MQ=255)                                                                                                                                                                                         tcgtcATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTAACTTATcc                                                            >  1:493587/1‑250 (MQ=255)                                                                                                                                                                                               tttGATTTCCTGACAACTGCCATCAAACAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGAGATTTAAGCCAGTTACTGAATGCCAAAATCATAAGCCCCAGCGCGCAAAAGCGGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTGTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATAGCTGCCTTATCCAgcgct                                                      <  2:14946/250‑2 (MQ=255)                                                                                                                                                                                                       ccTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGCATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGcc                                             >  2:20338/1‑251 (MQ=255)                                                                                                                                                                                                                 gCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGa                                   <  1:14300/251‑1 (MQ=255)                                                                                                                                                                                                                   cATCAAGCAACAGGGCTGCACGCTACAGCAGGCAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTATAGCCAGGTTGACGAGc                                 >  2:101090/1‑251 (MQ=255)                                                                                                                                                                                                                           aaCAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATc                         <  1:437141/251‑1 (MQ=255)                                                                                                                                                                                                                             aaGGGCTGCACGCAACAGCAGGTGGCTGATGCCAGCGGGATGACCAAAGGGACTTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGAAAAAGCTGGAGGCGTTGAACCGTTTTTTGAGGCTTGAGTTTCCACGGCCGAAGTAAACCATTGGTGTGCTATTCGGAAAATTCTACCCGCTGCTTACCGGACATATCTACCTTATCCAGCGCGCCTGTCGCCAGGCAGACGAACTGAATATCAt                       >  2:268277/2‑251 (MQ=255)                                                                                                                                                                                                                                             agcagGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGAt       >  1:414271/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCg  >  1:139696/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         CGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCG  >  NC_000913/4627134‑4627625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑