Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	6,239	G→T	A74E (GCG→GAG)	yaaA ←	DNA binding and peroxide stress response protein YaaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	6,239	0	G	T	85.7%	113.5 / ‑4.8	51	A74E (GCG→GAG)	yaaA	DNA binding and peroxide stress response protein YaaA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  major base T (31/11);  minor base C (0/7);  total (32/19)
Fisher's exact test for biased strand distribution p-value = 3.70e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATG  >  NC_000913/5992‑6484                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      gTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCg                                                                                                                                                                                                                                                    <  1:68106/251‑1 (MQ=255)     aGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGt                                                                                                                                                                                                                                                >  2:193030/1‑251 (MQ=255)          gttgGGGATGATATCCCCCCAGAATTGATCCAGATCTTTCCCTCGGGCATTCTCAAGCCGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATTCAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAAt                                                                                                                                                                                                                                           <  2:96759/251‑1 (MQ=255)            tgGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATc                                                                                                                                                                                                                                          <  1:249480/250‑1 (MQ=255)                        ctcCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTc                                                                                                                                                                                                                              >  1:499574/1‑250 (MQ=255)                                     aTACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGATGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCAGCCAGTCATGAACGCGAgcgg                                                                                                                                                                                                                >  1:341734/1‑251 (MQ=255)                                                         cATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCCTGAAAGCGAGCGGCGTTGATACCCGcccggttt                                                                                                                                                                                            >  2:407829/1‑245 (MQ=255)                                                               cAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCCGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTg                                                                                                                                                                                      >  2:323918/1‑251 (MQ=255)                                                                  gACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCAATACAAGCCGAAAAGCATTCGCAAATGCTTTTGGGCAAAATAGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCCCCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCGGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATg                                                                                                                                                                                   <  1:328055/251‑1 (MQ=255)                                                                      gATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCCTTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGTTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATgcgcc                                                                                                                                                                               >  2:15856/1‑250 (MQ=255)                                                                          cccATTTACAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCCTTCGAAAATGCTGTTGGGCAAGATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGCAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTATCCGGCGTGAAATCTGGCTGCCAGTAATGAAAACGGGCGGCGTGGCTACCCGCAAGTTTGTCACTggcgcgcaatac                                                                                                                                                                           >  2:165391/1‑240 (MQ=255)                                                                                  cAGACGATAAGGCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTa                                                                                                                                                                    >  1:427860/1‑250 (MQ=255)                                                                                           aGGCTGCATTAAACCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCCCCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAg                                                                                                                                                          <  1:251580/251‑1 (MQ=255)                                                                                             gCTGCATTAAATCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGc                                                                                                                                                        >  1:243560/1‑251 (MQ=255)                                                                                                       aTCGAGCGGGCGCAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTAATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCCTCAGCGTGCTAATCTGCGGCGGCGTCAGTTTcc                                                                                                                                              >  2:361676/1‑251 (MQ=255)                                                                                                                      cccGCCATAAAAGCAGAAAAGCATCCGAAAATGCTGTTGGGAAAAATC‑AAATCTGGCGTCGCGGAAGGTTTCGGCCTGAAAGCCGGTGTAGACACCCCCTTAAAACGCCAGAATCGCCGGGCGGGCATTCCCCGGGGTGAAATCGGGCTGCCAGTCAGGAAAGCGGGCGGCGTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCa                                                                                                                               <  1:323918/249‑1 (MQ=255)                                                                                                                           cATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCt                                                                                                                                                                >  1:494613/1‑213 (MQ=255)                                                                                                                              aCAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGGTGCGCATCAGCGTGCTAATCTGCGGCGGCGGCAGTTTCCGCGCTTCAGGGATCAACTGCTgg                                                                                                                       >  2:105949/1‑251 (MQ=255)                                                                                                                              aCAACCCGGAAAGCATTCGCAAAGGTTGTTGGGCAAAATCGAAACC‑GTCTGCGCTGAAGGTTTCGGCCGGAAAGCCGGTGTAGACATCCCCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTgg                                                                                                                       <  2:197967/251‑1 (MQ=255)                                                                                                                                   ccGGAGAGCATTCGCGAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGACGCGCATCAGCGTGCAACTCTGCGGAGGCGTCAGTTTCCGCGCTTACTGGATCAACTGCTGGGagtt                                                                                                                  >  2:146452/1‑248 (MQ=255)                                                                                                                                   ccGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAAtt                                                                                                                  >  2:287518/1‑251 (MQ=255)                                                                                                                                      gAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTc                                                                                                               >  1:34701/1‑251 (MQ=255)                                                                                                                                           cATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGCTGCGCATAAGCGTGCTCATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGATTTGTCTAAc                                                                                                           >  2:342425/1‑250 (MQ=255)                                                                                                                                            aTTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAAc                                                                                                           >  1:450195/1‑249 (MQ=255)                                                                                                                                             ttCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGc                                                                                                        >  1:505208/1‑251 (MQ=255)                                                                                                                                                    aTGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGCGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGcc                                                                                                 >  2:303798/1‑250 (MQ=255)                                                                                                                                                     tGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGc                                                                                                  >  1:32944/1‑249 (MQ=255)                                                                                                                                                      gCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAg                                                                                                <  1:193030/250‑1 (MQ=255)                                                                                                                                                                 aaatcgaaatc‑gTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCCGCGTATAGcgcgg                                                                                    >  2:247524/1‑250 (MQ=255)                                                                                                                                                                    ccaaaacc‑gTCTTCGCGGAAGGTTTCGGCCTGCAAGCGGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCgtggt                                                                                 <  1:247524/244‑1 (MQ=255)                                                                                                                                                                      gaaatc‑gTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGt                                                                                                                >  1:495442/1‑218 (MQ=255)                                                                                                                                                                                       aagtttccgcccTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAACCGCCGGGCGGGCATTCCCCGGTGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTa                                                               <  1:458533/241‑1 (MQ=255)                                                                                                                                                                                              cGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCCGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGc                                                        >  2:142946/1‑251 (MQ=255)                                                                                                                                                                                                gCCTGAAAGCCGGTGTAGACACCCCCTTTAAACGCCAGAATCGCCTGGCGGGCATTCCCGGGCGTGAAATCTGGCTGCCAGTAATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGt                                                      <  1:142946/251‑1 (MQ=255)                                                                                                                                                                                                cccTGCAAGCCGGTTAAGACATCGCCTTTAAACTCCAGAATCGCTGGGCGGGCATTCTCCGGCTTAAAACCGGGCTGCCAGTCATGAAGGCGAGCGGCTTTTATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGt                                                      <  1:490619/250‑1 (MQ=255)                                                                                                                                                                                                 ccTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCCGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGAGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGGATCGCGCGTGGTGGTCCACGGGCTTTGGGACTCAAGAGtt                                                     >  2:269406/1‑251 (MQ=255)                                                                                                                                                                                                          cGGTTTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGGGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGt                                            <  1:209265/251‑1 (MQ=255)                                                                                                                                                                                                              ggagaaaTCACCTTTAAACGCCAGAATCGCCTGGCGGCCATTCTCCGGCGTGACATCTGGCTGCCAGTAATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGACGCCGGTGTGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTCACAGCTCCGGCAGAGTATACCGAGTGGTGGTCAAAGGGGCTTGGTACTCACGCGTTTTCGCAgcgaaa                                         >  2:496934/7‑245 (MQ=255)                                                                                                                                                                                                                agaaaTCACTTTTAAACGCCATAATCGCCTGGCGGGCATTCCCCGGCGTGAAATCGGGCTGCCAGTCATGAAAGCGAGCGGCTTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATa                                      <  1:268673/247‑1 (MQ=255)                                                                                                                                                                                                                aGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGACATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGCGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCACCGGGCTTTGGTAATCAAGCGTTTTAGCAGGGGAAAt                                       >  2:448237/1‑250 (MQ=255)                                                                                                                                                                                                                 gACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGtttt                                                   >  1:110328/1‑237 (MQ=255)                                                                                                                                                                                                                    aTCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGa                                   >  1:123300/1‑250 (MQ=255)                                                                                                                                                                                                                      ccccTTTAAACGCCAGATGCGCCTGGCGGGCATTCTCCGGGGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATc                                <  1:361003/249‑1 (MQ=255)                                                                                                                                                                                                                       cccTTTAAACGCCAGAATCGCCTGGCGGGCATTCTCCGGCGGGAAATCTGGCTGCCAGTCAGGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCa                               <  1:303798/250‑1 (MQ=255)                                                                                                                                                                                                                           ttAAACGCAAAAAGCGCCGGGCGGGTATGCTCCGGCGTGAAATTGGGCTGCCAGTCATGAAGGCGGGCGGCTTTGATCCCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCGGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCa                            <  1:342425/250‑1 (MQ=255)                                                                                                                                                                                                                               aCGCCAGAATCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCCGGTGAAATAAGAATCAGCATATcc                       >  1:467189/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaTCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCtt                >  1:47108/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaTCGCCTGGCGGGCATTCTCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCtt                >  1:300264/1‑251 (MQ=255)                                                                                                                                                                                                                                      aaTCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCa                                                           >  1:185967/1‑208 (MQ=255)                                                                                                                                                                                                                                                   gCATTCCCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTAt   <  1:46147/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cATTCCCCGGCGTGAAATCTGGCTGCCATTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCTTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATg  <  1:361676/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      GTTCAGCTTGTTGGTGATGATATCTCCCCAGAATTGATACAGATCTTTCCCTCGGGCATTCTCAAGACGGATCCCCATTTCCAGACGATAAGGCTGCATTAAATCGAGCGGGCGGAGTACGCCATACAAGCCGGAAAGCATTCGCAAATGCTGTTGGGCAAAATCGAAATC‑GTCTTCGCTGAAGGTTTCGGCCTGCAAGCCGGTGTAGACATCACCTTTAAACGCCAGAATCGCCTGGCGGGCATTCGCCGGCGTGAAATCTGGCTGCCAGTCATGAAAGCGAGCGGCGTTGATACCCGCCAGTTTGTCGCTGATGCGCATCAGCGTGCTAATCTGCGGAGGCGTCAGTTTCCGCGCCTCATGGATCAACTGCTGGGAATTGTCTAACAGCTCCGGCAGCGTATAGCGCGTGGTGGTCAACGGGCTTTGGTAATCAAGCGTTTTCGCAGGTGAAATAAGAATCAGCATATCCAGTCCTTGCAGGAAATTTATG  >  NC_000913/5992‑6484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑