| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,626,601 | G→T | P230P (CCG→CCT) | radA → | DNA recombination protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,626,601 | 0 | G | T | 93.9% | 199.9 / ‑2.1 | 68 | P230P (CCG→CCT) | radA | DNA recombination protein |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base T (26/36); total (29/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGG‑CACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGG > NC_000913/4626356‑4626845 | gACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGt < 1:66870/251‑1 (MQ=255)gACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGG‑CACGTAACCAAAGATGGTTCGCTGGCTGGCCCGaaa < 1:141425/249‑1 (MQ=255) aCAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGG‑CACGTAACCAAAACTGGCTCGCTGGCGGGCCCCAAAggg > 1:81246/1‑249 (MQ=255) cAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGa < 1:411433/249‑1 (MQ=255) cAATATGTAGTCGGAAACCAGCATCGAGCAGAGCTGCCTGATTGCCGAAGAAGAGCATCCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTTCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGTCGATTGTCATGGTTGGA‑CTCGTAACCAAAGATGGCTCGCTGGCGGGTCCTAAAGTGCTg < 1:56120/247‑1 (MQ=255) cAATATGGTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTg < 1:418521/247‑1 (MQ=255) atGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGCTCCAGGTGATGCATATGGCGGATGTACAGTCCTCGCCTTGCAGCGTGGCGCAGGTGCGTGAAACTGCGGCTTATATGACGCGCTTCGCCCAAACCCGCGGTGTGGCGCTTTTCATTGTTTGG‑CCCCTAACCCAAGATGGCTCCCTGGCGGGCCCTCAAGTGTTGGTACACtt > 2:38037/1‑250 (MQ=255) tgtCGGAAACCGGCATCGATCAGATCTGCCTCGTTACCGAAGAAGAGCAACCGAAGCTGATGGTAAGTGACTCTGTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAATGTGCTGGAACACTGTAt < 2:217997/250‑1 (MQ=255) aTCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGAAGCGCTTCGCCCAAACGCGCGGTGTGGCGGTTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGTCGGGCCCTAAAGGGCTTGAAAACTTTATAGGCTGTTCGGTGCt > 1:204899/1‑251 (MQ=255) gcAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGGGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTgg < 1:284157/250‑1 (MQ=255) gccggATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATTCATATGGCGGAGGTACATTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCg < 2:283149/245‑1 (MQ=255) ccGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTcc < 1:206336/251‑1 (MQ=255) gagCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCg < 1:38037/251‑1 (MQ=255) gagCAACCGAAGCTGATGGGAATTGACTCGAGCCAGGTGATGCATATGGCGGATGTACAGTCAGCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGTCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCGCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGGTCGGTGCGTTTGGATGGCGATGCCGACTCCCGTTTTCg < 2:75371/251‑1 (MQ=255) aCCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGGTTTTGGATGGCGATGCCGACTCCCGTTTTAGCActt > 1:304387/1‑249 (MQ=255) aagtggagggTAAGTGACTCGATACAGGTGATGCATATGGCGGAGTTACAGTCAGCGACGGGCAGCGTGGCGGAGGTGCGTGAAGAGGCGGCTTAGCTGACGCGCTTCGCCAAAACGCGAGGTGTGGCGATGGTCATGGGTGGA‑CACGTAACCAAAGATGGCTCGCGGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCTGTTTTCGCAcgttacc < 2:101316/243‑7 (MQ=255) gCTGATGGTAATTGACTAGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTTTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTTGAACACTGTATCGCCTGTTCGGGGCTTTTTGGTGGCGATGCCGACTCCCGTTTTCGCActtttcgc > 1:356192/1‑243 (MQ=255) gtgaaTTGACTCGTTCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGTA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACTCTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGTCTCCCGTTTTCGCACTTTACGCAGCCAt < 1:278031/247‑1 (MQ=255) gacccGATCCAGGTGAGGCATATGGCGGATGTACCTGCACCGCCTGGCAGCGTGGCGCAGGGGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAAcc < 2:12537/246‑1 (MQ=255) ccGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCtt < 1:415845/250‑1 (MQ=255) cGATCCAGGTGATGCATAGGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTc < 2:86818/251‑1 (MQ=255) ccAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCGGACGCGCTTCTCCAAAACGCGCGGTGTGTCGATTGTCATGGTTGGA‑CCCGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGgcg < 1:281096/251‑1 (MQ=255) ccAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGGGAAACGGCGGCTTATCTGACGCGCTTCGCCCAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGGGCTGGGACACTTTATAGACTGTTTGGTGCTTTTTGGTGGGCAGGCCGACCCCCGTTTTCGCACCTTTCGCAGCCATAAAAACCCCTTCgggg > 2:263853/1‑249 (MQ=255) gTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCCAAAGTGCTTGAACACTGGATCGACTGTTCGGTGCTTTTGGGTGGCGATGCCGCCCCCCGTTTTCGGCCTTTACGCAGCCATAAAAACCGCTTCGGCGCggg > 1:70731/1‑250 (MQ=255) ggATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCg > 1:395397/1‑251 (MQ=255) ggATGTACAGGCATCGCCTGGCCGCGTGGCGCAGGTGCGTGAAACGGCTGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATGGTCATGGTTGGA‑CACGTAACCAAAGATGGCGCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATTCCGACTCCCGTTTTCTCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCg < 2:414208/251‑1 (MQ=255) cATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATg > 1:501662/1‑251 (MQ=255) cGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGGGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCTACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGAcc > 1:176752/1‑251 (MQ=255) gTAGCGTGGCGCAGGTACGTGAAACGGCGGCTTATTTGACACGCTTCGCCACAACGCGCGGTGTGGCGATTGTCATGGTGGGG‑CACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGAGGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAAc > 1:242143/1‑249 (MQ=255) caGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGAt > 1:127043/2‑163 (MQ=255) caGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGAt < 2:127043/162‑1 (MQ=255) aGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAggg < 1:192747/251‑1 (MQ=255) gCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAgggg > 1:227028/1‑251 (MQ=255) gcgcAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCg < 1:73599/251‑1 (MQ=255) cAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTg > 1:357240/1‑250 (MQ=255) gCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTc < 1:14916/250‑1 (MQ=255) tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGGAGGCAGc > 1:353413/1‑250 (MQ=255) tGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGc > 1:396639/1‑250 (MQ=255) aCGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTTGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAccc > 1:128835/1‑251 (MQ=255) aCGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTGTACGCAGCCATAAAATCCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAAccc < 1:485927/251‑1 (MQ=255) gggctgttttGACCCGCTTCGCAAAATCGGGCGTGGTGGGTATTGTCA‑GGTGGGGGCACGTAACCAAAGATGGTCCGCGGGCTGGCCCGAAAGTGCTGGATCCCTGTATCGACTTTTCGGTGCTTTTGGATGGCGATGCCGACCCCCGTTTTCGCCCCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTAGGCGTCTTCGCGATGACCGACCAGGGGCTTCGTGAAGTCAGCAACCCTTCg < 2:242143/244‑1 (MQ=255) actGACGCGCTCCGCCAAAACTCGCGGTGTGGCGATTGTCATGGTTGGA‑CACTTATCCAAAGATGGCTCGCTGGCGGGCCCTAAAGGTCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGGGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCATCGGCAAtt < 2:219252/248‑1 (MQ=255) cgcGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGGGCTTTTGGATTGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGACTGAACTGGGCGTCTTCGCGATGACCGAACAGGGGGTGCGGGAAGTCAGCCAACCTTCGGCAATTTTCtt > 2:424515/3‑250 (MQ=255) cgcGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTAGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCCTAAAAACCCATTCGGCGCGGGGAATGAGCTGGGCGTCTTCGCGATGGCCGACCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCGATTTTCtt > 2:22313/3‑250 (MQ=255) gCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGt < 1:870/251‑1 (MQ=255) tCGCAAAAACGCGCGGTGAGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTcg < 2:185869/250‑1 (MQ=255) cGCCAAAACGCGCTGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTcgcg < 1:482654/251‑1 (MQ=255) aaaaCGCGCGGTGTGGCGATTGTCATGGGTGGA‑CACGTAACCAAAGATGGCTCGCTGGAGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAAACATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCCCGATGAACGACCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTACGTcgaggagg > 1:202048/1‑245 (MQ=255) aaaCGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGAt < 1:37313/251‑1 (MQ=255) aaCGCGCGGTGTGGCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGTGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATg < 1:220887/251‑1 (MQ=255) ggCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTTGGCGTCTTCGCGGTGACCGCACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGGGATGAAGTGACCTCCg > 2:146629/1‑251 (MQ=255) ggCGATTGTCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTCCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGtcctcc < 1:115357/250‑1 (MQ=255) tCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGACGTGACCTCCGGCAGCTcc > 1:249461/1‑250 (MQ=255) tCATGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTcc > 1:373548/1‑250 (MQ=255) aTGGTTGGA‑CACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGACGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGCTGAAGTGCCCTCCGGCAGCTcggg > 2:8641/1‑248 (MQ=255) ggttggacACGTCACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGATGGAACCCTGTAACGAATGTTCGGTGCTTTTGGATGGCGATGCCGACTACCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGAACGTACAGGGGCTGCGAGAAGTCAGCAACCCTTCGGCCATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCggccgctccgtgg > 2:240669/8‑240 (MQ=255) aCGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCt < 1:137280/204‑1 (MQ=255) aCGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGGGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCt > 2:137280/1‑204 (MQ=255) aaCCAAAGATGGCTCTCTGGTGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTTCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGGCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTggg < 2:70731/251‑1 (MQ=255) aaCCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGCTGGTGGTGTggg > 1:321089/1‑251 (MQ=255) aCCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGgga < 2:396639/251‑1 (MQ=255) aaGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGggaagga < 1:263853/251‑1 (MQ=255) gATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGAGGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAAc < 2:124382/251‑1 (MQ=255) ggctCGCTGGCGGGGCCTAAAGGGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACGTGACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGGGGTGTGGGAAGGAAcgcg < 2:81246/248‑1 (MQ=255) ggcgggcCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTccgttgc > 1:348109/5‑246 (MQ=255) ggcgggcCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTccgttg < 1:326786/246‑5 (MQ=255) gcgggcCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTccgttgct < 2:249461/248‑7 (MQ=255) gggcCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCGGCAGCTCCGTGATGGGGGTGTGGGAAGGAAAGCGTCCGTTGCtgg > 2:476498/2‑251 (MQ=255) | GACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGG‑CACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAGCTCAGTGATGGTGGTATGGGAAGGAACGCGTCCACTGCTGG > NC_000913/4626356‑4626845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |