Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,242	T→C	D116D (GAT→GAC)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,242	0	T	C	96.1%	151.6 / ‑3.6	52	D116D (GAT→GAC)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (28/21);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

ACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTG  >  NC_000913/4624995‑4625487                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      actcAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGAGAATCAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATg                                                                                                                                                                                                                                                    <  2:499212/248‑1 (MQ=255)  cGCAGGTGGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATgg                                                                                                                                                                                                                                                   <  2:7091/251‑1 (MQ=255)               ggctggctGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCAACTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCTCCAt                                                                                                                                                                                                                                      >  1:258279/1‑251 (MQ=255)                     ctgctgTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATcc                                                                                                                                                                                                                                    >  2:324848/1‑247 (MQ=255)                              ggTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCGAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCCACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCCCGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCCTCCAGATTGAATGTAt                                                                                                                                                                                                                       >  2:151433/1‑251 (MQ=255)                                 cGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATtg                                                                                                                                                                                                                     <  1:324848/250‑1 (MQ=255)                                        tGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAAtt                                                                                                                                                                                                             >  2:154791/1‑251 (MQ=255)                                          gATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCGCATTAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGGTTGTTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGc                                                                                                                                                                                                           <  1:461333/251‑1 (MQ=255)                                                 agcGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTg                                                                                                                                                                                                    >  1:157111/3‑251 (MQ=255)                                                 agcGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGCGATTGTTGCCGCCTGGCGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGCTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGAGCAACTGGATGCCGCACCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGCGATGGACATGGATTCAACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTg                                                                                                                                                                                                    >  1:347024/3‑251 (MQ=255)                                                     ccTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCCCCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCg                                                                                                                                                                                                <  1:423581/250‑1 (MQ=255)                                                             aaTACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGc                                                                                                                                                                                        >  1:113615/1‑251 (MQ=255)                                                                   agagCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGAtg                                                                                                                                                                                  <  2:162532/251‑1 (MQ=255)                                                                       cAAACTGGGTGCGGCGATGGTGATTGTGGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGAtggtgg                                                                                                                                                                              <  1:421558/251‑1 (MQ=255)                                                                        aaaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGc                                                                                                                                                                             <  1:114399/251‑1 (MQ=255)                                                                         aaCTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCg                                                                                                                                                                            <  1:175268/251‑1 (MQ=255)                                                                               gTGCGTCGATTGTGATTGTTGCCGCCGGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTa                                                                                                                                                                      <  1:500240/251‑1 (MQ=255)                                                                                tGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCCCCGCACGGGCTCCGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAgga                                                                                                                                                                      >  2:69579/1‑248 (MQ=255)                                                                                 gcggcgATGGTGATTGTTGCCGCCTGGGGCGTGGAAGATGATCAGGTGATTCGGCTGGCAGGTGCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTaa                                                                                                                                                                     <  2:234971/250‑1 (MQ=255)                                                                                      gATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAc                                                                                                                                                               >  1:33711/1‑251 (MQ=255)                                                                                           ggATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGct                                                                                                                                                          <  1:380313/250‑2 (MQ=255)                                                                                            gATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGCACGGGCgg                                                                                                                                                         >  2:161219/1‑250 (MQ=255)                                                                                                        cTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAAc                                                                                                                                             >  1:215092/1‑251 (MQ=255)                                                                                                                         aTCAGGGGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTAcagcacgc                                                                                                                            >  2:362486/1‑244 (MQ=255)                                                                                                                          tCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGGGATGGTGGCGGAAGTAACCGAACGGGGGATGCGCGGCGAACTCGCTTTTACCGCCAGcc                                                                                                                           >  2:185881/1‑251 (MQ=255)                                                                                                                            aGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTg                                                                                                                         <  1:259566/251‑1 (MQ=255)                                                                                                                              gTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTgcg                                                                                                                       >  1:108666/1‑251 (MQ=255)                                                                                                                               tGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCGTGATGCGCATCTGGCTGTCGCCCCGCTGGGGCAAATCCCGCACCTGCGCACGCAGGGTTTGATAGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGAGCGAGCTGGTGGCGGAAGTAACCGAACGTGCGATGCGCGGCGAACTAGATGTTACCGCCAGCCTgcgc                                                                                                                      >  1:452102/1‑251 (MQ=255)                                                                                                                                    cGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCGGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGcc                                                                                                                  <  1:422864/250‑1 (MQ=255)                                                                                                                                      tCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGACAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACCCGGTTTTACCGCCAGCCTgccccgccgcg                                                                                                               >  2:449862/1‑242 (MQ=255)                                                                                                                                       cTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCgcgt                                                                                                              >  1:60887/1‑248 (MQ=255)                                                                                                                                           ccggTTCACTCACCGCACGGGCTACGCGCCTGGCCCGAGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAAGCCCGCCCCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCg                                                                                                          <  1:208754/249‑1 (MQ=255)                                                                                                                                                   ctcacCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGACTGGGCAGCCGCGAGGCGACGcggac                                                                                                  >  2:272965/1‑247 (MQ=255)                                                                                                                                                          cACGGGCTACGCGCCTGGCCCATGACGAGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGCGCTGGCCATGGCTTCCACCGCCATCCAGATTGCATGAATTGATGAAATTGCCAAACTGGTCGGGACGGGAGCGATGGTGGCGGGAGTAACCGAACGGGCGAAGCGCGGCGAACTCGATTTTCCCCCCAACCTGCGCAGccacgggacgccgctgaaaggggat                                                                                           >  2:279469/1‑228 (MQ=255)                                                                                                                                                             gggCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGcc                                                                                                                                                                                                          <  2:140238/137‑1 (MQ=255)                                                                                                                                                             gggCTACACGCCTGGACCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGcc                                                                                                                                                                                                          >  1:140238/1‑137 (MQ=255)                                                                                                                                                                         gggCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAACCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCCCCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTg                                                                            <  1:161219/250‑1 (MQ=255)                                                                                                                                                                          ggCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCCATATTCTGc                                                                           >  2:481666/1‑251 (MQ=255)                                                                                                                                                                           gcccatgaggcgcacctggagtgcGCCCCTCGGGGGAAAACCCCGCACCTGCGCACGCGGGGTTGGCTGGTGATGGACATGGTTTCCACCGCCATCCAGATTGAAGGTATTGATGAAATTGCCAACCTGGCCGGAACGGGCGAGAGGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCa                                                                          <  2:148632/228‑1 (MQ=255)                                                                                                                                                                              catgaagcgcaatgggaggTCGCCCCGCTGGGGAAAATCCCGCACCGGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCCCCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACGCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACa                                                                       <  2:215092/233‑1 (MQ=255)                                                                                                                                                                                      gcaacTGGAGGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATGGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTg                                                               <  1:457862/247‑1 (MQ=255)                                                                                                                                                                                                     cccGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGCTGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTg                                                 >  1:284756/1‑250 (MQ=255)                                                                                                                                                                                                             ggAAAATACCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTCTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGc                                        >  1:329291/1‑251 (MQ=255)                                                                                                                                                                                                               aaaaTCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGGGCGTGAAAATCTGCCGCTGATGCCAGGCtt                                      >  2:132375/1‑251 (MQ=255)                                                                                                                                                                                                                   ccccGCACCAGCGCACGGGGGGTTTGCTGTTGATGGACTTGGATTCCACCGCCCTCCAGATTGAAGGTATTGATGAAATGACCAAACTGGCCGGAACGGGCGAGATGGTGGGGGAAGTAACCGAACGGGCGATGCGCGGCGAACCCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGTTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTCACg                                  <  2:93015/250‑1 (MQ=255)                                                                                                                                                                                                                       gCACCTGCGCACGCCGGGTTTGCTGGGGATGGACATGGATTCCACCGCCATCCAGATTGCATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGAGGCGGAAGTAACCGAACGGGCGATGCGCGCCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGACGGGCGCTGACGCCCATATTCTGCAACAGGGGCGTGAAAATCTTCCGCGGATGCCCGGCTTAACGCAAc                              >  2:69211/1‑251 (MQ=255)                                                                                                                                                                                                                        ccccTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACt                             <  1:490294/249‑1 (MQ=255)                                                                                                                                                                                                                         aCCTGCGAACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGCTTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGGACGGGCGATGCGAGGCGAACTCGATTTTACCGCGGGCCTGCGCAGCCGCGTGGCGACGCTGAAAGACGCTGACGCCAATATTCTGCCAAAGGTACCTGAAAATCTGCCGCAGATGcccgcagtaaggcaacgg                            >  2:15678/1‑235 (MQ=255)                                                                                                                                                                                                                              ggcACGCCGGGTTGGCTGGTGATGGAGATGGATTCCACCGCCATCCAGATTTAATGTATTGATGAAATGGCAAACCGGGCGGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCt                       <  2:200911/250‑1 (MQ=255)                                                                                                                                                                                                                                cACGACGGGTGTGCTGGTGATGGACCTGGATTCCACCGCCATCCAGATTGAATGAATTGATGAAATTGCCAAACTGGCCGGCACGGGCGAGGTTGGGGCGGAAGTAACCGAACGAGCGATGCGCAGCGAACTCGATTTTACCGCCCGACTGCGCAGCCGCGTGGCGACGCTGAAAGGAGCTGACGCCAATATTCTGCAACAGGTGCGTGACAATCTGCAGCTGATGCCAGGcatacctcgactggtacac                      >  2:75997/1‑232 (MQ=255)                                                                                                                                                                                                                                            gCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgc         >  2:412162/1‑251 (MQ=255)                                                                                                                                                                                                                                                   aTGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGCGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACgctgggctg  >  1:380973/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      ACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAACGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACGCTGAAAGGCGCTGACGCCAATATTCTGCAACAGGTGCGTGAAAATCTGCCGCTGATGCCAGGCTTAACGCAACTGGTGCTCAAGCTGGAAACGCTGGGCTG  >  NC_000913/4624995‑4625487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑