Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,044	A→G	Q50Q (CAA→CAG)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,044	0	A	G	100.0%	191.8 / NA	55	Q50Q (CAA→CAG)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/33);  total (22/33)

CTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGC  >  NC_000913/4624798‑4625286                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTc                                                                                                                                                                                                                                                >  1:153471/1‑251 (MQ=255)     gAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGAc                                                                                                                                                                                                                                            >  1:436564/1‑251 (MQ=255)     gAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGAc                                                                                                                                                                                                                                            <  1:346384/251‑1 (MQ=255)       aaaTTTGAGACTTAATGGTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGAccc                                                                                                                                                                                                                                          <  2:163819/251‑1 (MQ=255)                    aaTGTAGCCAGAAGCAATGGATACAAGGTAGCATCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAAGAGCCTTAATTCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCTGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCAAATACCAGAGCaa                                                                                                                                                                                                                             <  2:302445/251‑1 (MQ=255)                       gTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACt                                                                                                                                                                                                                          <  1:157475/251‑1 (MQ=255)                          gCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTggg                                                                                                                                                                                                                       <  1:96660/251‑1 (MQ=255)                                       gATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTg                                                                                                                                                                                                          >  1:443603/1‑251 (MQ=255)                                          aCAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGAtt                                                                                                                                                                                                       <  1:179921/251‑1 (MQ=255)                                           cAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGAttg                                                                                                                                                                                                      <  2:442795/251‑1 (MQ=255)                                            aaGGTTGCCTCATGCGTGATTTTCCCGGCTTCGAACGAGTTTACAGGAGCCTGAATGCCTAACATTACCTGGTGCGGCCTGCCTGAAGATTTCTCTTTATGGCCTGGTCTGCCTCTTTCATTAAGTTGTGATGAAGTGATGCCTCTGGATTACCACGCAGGTCGGAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGATCAAACTGGGTGCTGCGATGGTGAttgt                                                                                                                                                                                                     <  2:311513/251‑1 (MQ=255)                                                           gttatttttccttatgcGAACGATTTTACAGGAGCCTTAAGGCCTAACCTTACCTGGTGCGACCTGCCTGAAGATGTCGTTTTAGGTCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGGGAAGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGt                                                                                                                                                                                      <  2:279492/235‑1 (MQ=255)                                                                 ttCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACCTTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATACACAGCGTCTGACACAATCCCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTggc                                                                                                                                                                                   >  1:279416/1‑247 (MQ=255)                                                                    ccTGCTTCGAACGATTTTACAGGAGCCTTAAGGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTa                                                                                                                                                                             <  2:330358/251‑1 (MQ=255)                                                                          gcGAACGATTTCACAGGAGCCTTAATGCCTAACATGACCGGGTGCGCCCTGCCTGAAGATGTCTCTTTATGTCCGGGTCTGCCTCTTTCATTAAGGGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTTCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAg                                                                                                                                                                         <  2:391191/248‑1 (MQ=255)                                                                            gAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGCCCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTTCCGCCTGGTGCGTGGGAGATTATccgggg                                                                                                                                                                      >  2:265282/1‑245 (MQ=255)                                                                                    ttACAGGCGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAATATGTCTTTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCt                                                                                                                                                              <  2:479738/250‑1 (MQ=255)                                                                                     tACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTgg                                                                                                                                                            >  1:200911/1‑251 (MQ=255)                                                                                           aGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAACCAGCGTCTGACCCCATACCAGCGCAAACTGGGTGCGGCGATGGTGATTATTGCCTCCTGGAGCGTGGAAGGTTATAAGGTGATTCGTCTGGCAGGtt                                                                                                                                                      >  1:172523/1‑251 (MQ=255)                                                                                                 aaTGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCGCGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtcactca                                                                                                                                                <  1:30208/251‑1 (MQ=255)                                                                                                  aTGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtcactcac                                                                                                                                               >  1:126360/1‑251 (MQ=255)                                                                                                    tcccaaCATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTGCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCAcc                                                                                                                                              <  2:158869/246‑1 (MQ=255)                                                                                                         aCATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACgg                                                                                                                                        <  1:426247/251‑1 (MQ=255)                                                                                                               ccTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTacg                                                                                                                                  >  1:148632/1‑250 (MQ=255)                                                                                                                  ggTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTacgcg                                                                                                                                <  1:422818/250‑4 (MQ=255)                                                                                                                     gCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTg                                                                                                                            <  2:33500/251‑1 (MQ=255)                                                                                                                          ctgcctgAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTg                                                                                                                                                                                         <  2:755/185‑1 (MQ=255)                                                                                                                          ctgcctgAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTg                                                                                                                                                                                         >  1:755/1‑185 (MQ=255)                                                                                                                             cctgAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCatga                                                                                                                    <  1:265282/251‑4 (MQ=255)                                                                                                                                gAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGGTCACTCACCGCACGGGCTACGCGCCTGGcccctgaagc                                                                                                                 >  1:312086/1‑244 (MQ=255)                                                                                                                                      gTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGCAGTGATGCCACTGGATTACCACGCCGGTCGTCGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAACACCAGAGCAAACTGAAGGCGGCGATGGTGATTGGTGCCGCCTGGTGCGTGGCAGCTTATCAGGGGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTTCGCGCCTGGcccctaacgcgcaacc                                                                                                           >  2:410689/1‑238 (MQ=255)                                                                                                                                                 gtcGGGTTTGCCTCTTTCATTAAGTGGGGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGccc                                                                                                <  2:387025/249‑1 (MQ=255)                                                                                                                                                          gCCTCTTTCATTAAGTGGTGATGAAGTGATGTCACTGGATGACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGa                                                                                       <  2:409125/251‑1 (MQ=255)                                                                                                                                                                ttCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAAt                                                                                   <  1:230011/249‑1 (MQ=255)                                                                                                                                                                 tcagtAAGGGGTGATTAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATcc                                                                                 <  2:506167/246‑1 (MQ=255)                                                                                                                                                                   aTTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTacg                                                                                                                                  >  2:409694/1‑198 (MQ=255)                                                                                                                                                                   aTTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTacg                                                                                                                                  <  1:409694/199‑2 (MQ=255)                                                                                                                                                                   aTTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAGGCGCAACTGGATGTCGCCCCGCTGGGGAAAATccccc                                                                              >  2:321889/1‑249 (MQ=255)                                                                                                                                                                      aaggggtgatgaagcgtggcccttGGATTACCACGCAGTTCGTAGCTGCTGGCTGCTGTATGTGCGTGGGCAGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACGCACCGCCCGGTCTACGCGCCTGGCCCATGAAGCGCAACGGGATGCCGCCCCGCTGGGGAAAATCCCGctc                                                                            <  2:298187/227‑3 (MQ=255)                                                                                                                                                                      aaGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCAcc                                                                           >  2:259566/1‑251 (MQ=255)                                                                                                                                                                       aGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGGGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGAACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCAccc                                                                          >  2:102668/1‑250 (MQ=255)                                                                                                                                                                                gaAGTGATGCCACTGGATTACCACGCAGGTCGTTGCGGCTGGCGGCTGTATGGTCGTGGGCTGGATAAACAGCGTATGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCCCCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGcc                                                                 <  2:7574/251‑1 (MQ=255)                                                                                                                                                                                gaAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTTGCTCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGcc                                                                 <  2:482513/251‑1 (MQ=255)                                                                                                                                                                                  aGTGATGCCACTGGATTACCACGCAGGGCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCgg                                                               <  2:109598/251‑1 (MQ=255)                                                                                                                                                                                         ccACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCCCGCCGGGTTTGcc                                                        >  2:383030/1‑250 (MQ=255)                                                                                                                                                                                              ggATTACCACGCAGGTCGTAGCGGCGGGCTGCTGTAGGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTTCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCAGGGTTTGCTGGTGa                                                   <  2:373991/251‑1 (MQ=255)                                                                                                                                                                                                      actcAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGAGAATCAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATg                                           <  2:499212/248‑1 (MQ=255)                                                                                                                                                                                                      aCGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGCTTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCCCCTGCGCACGCCGGGTTTGCTgggggtggacatg                                           >  2:422864/1‑240 (MQ=255)                                                                                                                                                                                                       cGCAGGTGGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATgg                                          <  2:7091/251‑1 (MQ=255)                                                                                                                                                                                                                    ggctggctGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCAACTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCTCCAt                             >  1:258279/1‑251 (MQ=255)                                                                                                                                                                                                                          ctgctgTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATcc                           >  2:324848/1‑247 (MQ=255)                                                                                                                                                                                                                                   ggTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCGAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCCACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCCCGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCCTCCAGATTGAATGTAt              >  2:151433/1‑251 (MQ=255)                                                                                                                                                                                                                                      cGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATtg            <  1:324848/250‑1 (MQ=255)                                                                                                                                                                                                                                             tGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAAtt    >  2:154791/1‑251 (MQ=255)                                                                                                                                                                                                                                               gATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCGCATTAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGGTTGTTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGc  <  1:461333/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGTTGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGAAATTGC  >  NC_000913/4624798‑4625286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑