| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,624,400 | C→T | E130E (GAG→GAA) | ytjB ← | putative inner membrane protein Smp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,624,400 | 0 | C | T | 96.5% | 164.2 / NA | 60 | E130E (GAG→GAA) | ytjB | putative inner membrane protein Smp |
| Reads supporting (aligned to +/- strand): ref base C (1/1); major base T (35/21); minor base G (2/0); total (38/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCG‑CAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCC > NC_000913/4624167‑4624646 | tcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTAAGTTTACCCTGTAGCAGCGTGCGGGGCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGGGTCGAGCGTCAGGCGCAGATAGCCGAGCGGGCCGTTTTTCCCCG‑CAATTGGGTCGACAATCTGCTgggt > 1:397622/1‑249 (MQ=255) cctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCCGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCCACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGGCCGTTTTTCCCCG‑CAATTGGTTCGACAATCTGCTGGttt > 1:177086/1‑250 (MQ=255) tcttccGGCACCGGTTTGCTGGCGGTTAACAGGCAGGGCGATTTCTGCCAGCGGGTACGTTTACCCTGTAGCAACGTGCGGGGCAGCACTACGCCGATTTCCAGTGAAAGCAGCAAAATCAGGCGTAAAATGTTTGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGGGTCGAGCGTCAGGCGCAGATAGCCGCGCGGTCCGTTTTTTCCGG‑CAATTTGGTCGACAAATTGCTGGttta > 1:8934/1‑248 (MQ=255) tcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAAACTGCTGGTTaa > 1:409397/1‑250 (MQ=255) gTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTTTTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCt < 1:188445/251‑1 (MQ=255) gggttaCAGGAATGGCGTTTGCAGCCAGCGGGTCCGTTTACCCTGTAGCAGCGTGGGGGTCAGCACTCCTCCGATTGCCAGGGAAAGCAGCAACATCAGGCGTTAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATTGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTTGACAATCTGCTTGTTAAAATAGCCGCCCGCTTTTTTACCGt < 2:238658/246‑1 (MQ=255) aaCAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCGGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGa < 2:136551/250‑1 (MQ=255) gggCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGcc > 1:228406/1‑251 (MQ=255) gcCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCa < 1:292156/249‑1 (MQ=255) ccAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACtt > 1:446837/1‑251 (MQ=255) cAGCGGTTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTc < 1:467263/251‑1 (MQ=255) aGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGAAAAATCAGGCGTAAAATGTTCGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGCGCGTATGGGTGTCGAGCGTCAGGCGCCGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAAATGGTTCGCCAATCTGCTGGTTCAAATAGCCGCCCGCTTTTTTACCGTCGACCGCCAGACGGTCGCGCACTTCg > 2:179921/1‑251 (MQ=255) gggTACGTTTACCCTGTAACAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGCGCGTATGGGTGTCGAGCGTCAGGCGCAGCTAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACACTCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACg > 1:32713/1‑251 (MQ=255) ccGTTTGCCCTGTAGCCGCGTGCGGGTCAGCACTGCGCGGATTGCAAGTGAAAGGAGCCACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTGGGTGGGGGGGGTATGGGTGGCGAGCGTCAGGCGCAGATAGCCTAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGTTTTTTTACCGTCGAGCGCCAGCCGGTCGCGAACTTCGACGCttt < 2:188910/250‑1 (MQ=255) gTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAACTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCCGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCTCTTTTTTCCCGTCGAGCGCCAGCCGGTCGCGCCCTTCGACGCTTTCg > 2:288855/1‑251 (MQ=255) tttACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCCCTTCGCCGCTTTCGc > 2:467163/1‑251 (MQ=255) aCCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTCCCgac > 2:124168/1‑211 (MQ=255) aCCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCgac < 1:124168/213‑3 (MQ=255) cTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATAAGGAGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCCCCAGAAc > 1:311513/1‑251 (MQ=255) gTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACgg < 1:172211/251‑2 (MQ=255) agcagcGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACgggc < 2:177086/251‑4 (MQ=255) gTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATa > 1:104233/1‑249 (MQ=255) cAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTa > 1:62348/1‑166 (MQ=255) cAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTa < 2:62348/166‑1 (MQ=255) cAGCACGACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCTCAAAATGTTAGTGGTGTTATCCACCTGTTGGGATTCGGTGGCGCGCGTATGGGGGTCGAGCGCCAGGCGCAGATAGCCGATCGGTACGTTTTT‑CCCGACAATAGGTTAGACAATCAGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGGGCGCCAGCCGGTAGCGCACTTCGACGCTTTCACCAGAACGGGCGATACGATCGccgg > 2:96660/1‑249 (MQ=255) aTTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCCTacac > 2:51677/1‑251 (MQ=255) ccAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGCACGGGCGATAAGATCGCCTTGTTCGTCATACACGccc > 1:227987/1‑251 (MQ=255) ccAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATacccgccc > 1:456749/1‑245 (MQ=255) ccAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGGGGTGTTATCCACCTGTTGGGCTTAGGTGGCGAACGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTAGACAATCTGATGGTTAAAATAGCCGCCCGCTTTTTTACCGTCATGCGCCAGCCGGTCGCGCACTTAGACGCTTTCTCCAGAACGGGCGATGAGATCGCCTTGTTCGCCATacacgcac > 2:346384/1‑247 (MQ=255) cAGTGAAAGCAGCAACATGAGGTGTAAAATGTTAGTGGTGTTATCCACCGGTTGGGCTTCGGTGGCGATCGTATGGGTTTCGAGCGGCAGGCGCAGATAGTCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcg < 2:399119/251‑1 (MQ=255) cAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCcg < 1:48515/251‑1 (MQ=255) gcagcaAAATCAGGGGGAAATTGTTTGTGGGTTTTTCCCCCTGTTGGGCTTCGGTGGCGGGTGGATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTTGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTGACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTGCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGGCGAgg < 2:67870/251‑1 (MQ=255) aaCATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGTGCTTCTGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGCGCGGTCCGTTTTTCCCGG‑CACTTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCCCTTCGACGCTTTCGCCAGAACGGGCGTTAAGATCGCCTTGTTCGTCATACCCGCCCCCGTCggagttacc > 2:146651/1‑243 (MQ=255) cGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTAGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCACGCGTCGAGGATACGGATTTCATc > 1:298187/1‑251 (MQ=255) tAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGCTACGGCTTTCATCCg > 1:479738/1‑251 (MQ=255) tAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCg > 1:80668/1‑251 (MQ=255) aaTGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCCGGCGCAGCTAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACACTCTGCTGGTTAACATAGACGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGCTCGCCTTCTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTTATTCGttt > 1:61068/1‑250 (MQ=255) ttAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGCGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGCCCGGGCGATAAGATCGCCTTGTTCGTCCTACACGACCGCCTCGAGGGTAGGGCtttacttcgttaaatg > 2:300441/1‑238 (MQ=255) aGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGAGCACTTCGCCGCTTTCGCCAGAACGGGCGATCAGATCGCCTTGATCGTCATACACGCCCGCGTCGAGGAAACGGCTTTCACCCGCTAACTgct > 2:145508/1‑249 (MQ=255) ttATCCACCTGTTGGGCTCCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCG‑CAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGa < 1:126835/250‑1 (MQ=255) tATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTCCCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGCACGGGCGATAAGATCGCCTTGTTCGTCATACAAGCCCGCGTCGAGGATACGCCTTTCATCCGTTagatgttcgggggtc > 2:350471/1‑237 (MQ=255) aTCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATc < 1:386374/250‑1 (MQ=255) aTCCACCGGGTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATc < 1:146651/250‑1 (MQ=255) aCCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCTGCCGGTCGCGCACTTCGACGATTTCGCCAGAACGGGCGATAAGATCGCCTTGGTCGTCATACACGCCCGCGTCGAGGGTACGACTTTCATCCGTTAACTgttagtggctcgcctg > 2:29241/1‑236 (MQ=255) gggCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCTCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATcc < 1:315481/250‑1 (MQ=255) ggCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCg > 1:387025/1‑251 (MQ=255) gggTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGATCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTa < 1:349370/251‑1 (MQ=255) cgtCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATACGATCGCCTTGTTCGTCATACACGCCCGCGCCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCg > 1:59572/2‑251 (MQ=255) aGGCGCAGATAGCCGAGCTGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGc < 1:168960/251‑1 (MQ=255) aGATAGCCGAGCGGTCCGTTTTTACCCG‑CAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCTTCGAGGATACGGCTTTCATCCGTTAACTGATCg > 1:403405/1‑192 (MQ=255) tcccgcgcgtgtcggttttctctg‑CAATTGGTTCGACAAGCTGCTGGTTAAAATAGCCGACCGCTTTTTTACCGGCGTTCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCAGCCGTTAACTGATCGAGGAGCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTCCAg < 2:8420/227‑1 (MQ=255) gAGCGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGtta < 1:350471/251‑3 (MQ=255) cGGTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGTTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCt < 1:482196/251‑1 (MQ=255) ggTCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTg > 1:499212/1‑251 (MQ=255) tCCGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTgcc > 2:315476/1‑249 (MQ=255) ccGTTTTTCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCCGCGGTGCAACGTTCAGCGTTACCTGAcc > 2:190731/1‑250 (MQ=255) tttttCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCAACCGGCGAGGTGGTCCGCATGAGCGGAGCacagttcaacgttaactgaggcgc > 2:328490/1‑228 (MQ=255) tttttCCCGG‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGACGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATACGGCGAGTCGGAACGCATCAGCGGTGAAACGTTCAGCGTTACCTGACgcgc > 1:25757/1‑251 (MQ=255) ccgg‑CAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAgggg > 1:409125/4‑250 (MQ=255) ttGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCCTACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGCTCGCCTGAATGCGTATTTCATCCGGCGAGTCGGTCCGCATCCGCGGTGCAACGTTCCGCGTTACCTGACGCGCCAGGGGGCGGGcc > 2:199629/1‑251 (MQ=255) | TCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCG‑CAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCC > NC_000913/4624167‑4624646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |