| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,624,037 | T→C | Q27Q (CAA→CAG) | lplA ← | lipoate‑‑protein ligase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,624,037 | 0 | T | C | 94.4% | 150.4 / ‑5.2 | 55 | Q27Q (CAA→CAG) | lplA | lipoate‑‑protein ligase A |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (21/30); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGA > NC_000913/4623800‑4624278 | gAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATac < 1:77477/251‑1 (MQ=255) acaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATacaccc > 2:107087/1‑249 (MQ=255) acaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGGTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCctc > 2:266737/1‑251 (MQ=255) cgtggggatggttttatctgtccccGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCTCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTCTCTGCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGGTCTGCGCGCGACCAATCACTAACGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCCTCCTCCCCCg < 2:324751/228‑1 (MQ=255) ttttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAc > 1:178044/1‑251 (MQ=255) tatcgagccacggctgtcacgacaTAAAGGTAAAGAGGGTATTGCCGAGTTGGTGGAACCGCGCGACTCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTACTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGAGACCAACCACTACCGTGTCGGCATTGCGACAGAGAAACA‑GAACGCGCTGCGTGGCGGGAATCAGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCa < 2:207035/228‑1 (MQ=255) tATCGTACCCCGCGTTGCCGGCCATAAAGGTAAAGCAGGTATGGCCGATAGTGGGGAACCCCGCTCAGCCACCGCTACCGCGCCGGGCCAGGCGGACGTTATCTTCTTCCACCCGCCGGGTATTACCCTCTTTCCACGGGTGCTGCGCGCGACCAAGCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCa < 2:399333/250‑1 (MQ=255) cggaCTCCGGCATGCCAGCCATAAAAGCAAAGAAGGTATTACCCGGATCGTGGAACAACGCGCCACCACAGCTACTGCGCCGTGCCACGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCAACCAATCACTCCCGTGCAGGCATTGCGCCAGAGAAACA‑GAACGCACTACGTGGCGGTCCTCTGGAGAAAAATACACCACTCCACCGCCAGGTTAACCCACggg > 2:25459/4‑251 (MQ=255) cGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTAATGCCGAGATCGTGGAACACCGCGCCGCCACAGCAACTACGCCGGGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTATGCGCGCGACCGATCACTACCGTGTGGGCATTGAGCAAGAGAAACATCAAC‑AGCCGCGGGTCGGGCATTGGGCTAAAAAGACACTCCCCCACCGTCAGGTTAAACAACggg > 2:54750/1‑251 (MQ=255) ggaCTCCGGCTTGCCAGACATAAAAGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACC‑GAACGCGCTGCGTGGCTGCCATTGGGCGAAAAATACACTCCTCCACCCCCAGTTTCAACCACGGGt > 2:349370/3‑251 (MQ=255) gTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGAGTGGCGGGCATCTGGCGAAAACTACACTCCTCCACCGCCAGGTTAAACCCCgggg > 2:48515/1‑250 (MQ=255) aCTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTc < 1:295980/250‑1 (MQ=255) cTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGt > 1:238658/1‑251 (MQ=255) acaGGTAACGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCAAGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCTTCTGGCGAAAAATACCCTCCTCCACCGCCAGCTTACAACACGGGTCGTAAGAGTCAGAGATGAGc > 2:188445/3‑250 (MQ=255) ggTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGc < 1:251912/251‑1 (MQ=255) cAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt < 1:107087/251‑1 (MQ=255) aTTGCCGAGATGGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCACCCGCCGGGTATTACACTCTTTCCCCGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa < 2:395830/251‑1 (MQ=255) gggtCGTGTAACTCCGCGCCGCCACCGCTACTGCGCCGTTCCAGGGGGACTTTATCTTCGTCCAGCCGCCGGTTATTCCACTCTTTCCACGGGTTCTGCGCGCGACCAATCGCTACCGTGTCGGAATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCGTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCATGCGTAATGTGGACAGAACGAt < 2:469461/248‑1 (MQ=255) aTCGTGGAACCCCGCGCCGCCCCCGCTCCTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt < 2:466244/251‑1 (MQ=255) tGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCCTTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGGGGACATAACGATTTCCtt > 1:17645/1‑251 (MQ=255) ggAACACCGCGCCGCCACCGCTACTGCGCTGTGCCAGGCGGCCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGGCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt > 1:404355/1‑251 (MQ=255) gcacccccccccgccTCCGCTACTGCGCCGTGCCAGGCGGGCGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc < 2:360170/241‑1 (MQ=255) gAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc < 1:17232/251‑1 (MQ=255) acCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgt < 1:415409/251‑1 (MQ=255) ccGCGCCGCCTCCGCTTCTGCCCCGTGCCAGGCGGACGTTATCTTCCTCCATCCGCCTGGTATTACTCTCTTTCCTCGGGTTCTGCGCGCTACCAATCACGACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACTCGCGGCGTGGCGGGCATCTGTCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgta < 1:227228/251‑1 (MQ=255) cgcgCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag < 1:266737/251‑1 (MQ=255) cgcgCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag < 1:172547/251‑1 (MQ=255) gcgcCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt < 1:238672/251‑1 (MQ=255) cgccgcCACCGCTACTGCGCCGTGCCAGGCGGACGTTATATTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGGTAAACCACGGGTCGTAAGAGTCAGGGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt > 1:175223/1‑250 (MQ=255) gccgccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa < 1:85598/251‑1 (MQ=255) gacggcgccgtgccAGGCGGACGTTACCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct < 2:178044/247‑1 (MQ=255) gtgccAGGCGGACGTTATCTGCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCg < 2:78397/249‑1 (MQ=255) gTTAGCTTCTGCCATCCGCCGTGTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATTAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTATTAATGGGTCACTCTTTTTTCTCGCTTtcttcctctt < 1:221015/251‑1 (MQ=255) tATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCCCGACCACTCACTACCGTCTCGGCATTGCGCCAGAGAAACA‑GCACGCGCTGCGTGGCGGGCATACGGCGAAAAATACACCCCTCCACCGCCCGGATAAACCACGGGTCGTCAAAGTCAGAGATGGGACGGCGTAATGTGGACATAACGGTTTCCTTGCTTGTTGGAATGGGGTACTCTTTTTTCTCGCTTtcttcctcttcc > 2:168960/1‑251 (MQ=255) aTCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCg > 1:259360/1‑251 (MQ=255) ccgccgGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGc > 1:312610/1‑251 (MQ=255) cagccgGGTCTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAAAGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACACCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAAATAAAGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCAGGCACCGGTTTGc > 1:312603/3‑251 (MQ=255) caccgGGGATTACACTCTTGCCACGGGTTCTGCGCGAGACCACTCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGATGCGTGGCGGGCCTCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGCAAGAGGCAGAGCTGGGCAGGCGTCATGAGGCCATAACGATTTCCTTTCTTGGAGATATGAGCCCCTCTTTTTTCTCGATTTCTTCCTCTTCACGCACCGGTTTGCt > 2:315481/3‑251 (MQ=255) cgGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGc > 1:273322/1‑251 (MQ=255) tATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGtt > 1:112182/1‑251 (MQ=255) tACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAc > 2:467263/1‑251 (MQ=255) acacTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCTGGCCTCTGGCGAAAAATCCACTCCTCCACCGCCCGGTTAACCCACGGGTCGTAATAGTCAGCGATACGCAGGCGTACTGTGATCATAACGATTTCCTTTCTTGTAGTAATGAGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGTCACCGATTTGCTGGCGGtttta > 2:383939/1‑247 (MQ=255) ttCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGTCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAgg < 1:200945/250‑1 (MQ=255) ccACGGGTGCTGCGCGAGTCCAATCTCGACCGTGGCGGCTGTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCGGGCGAAAAATTCACTCCTCCACCGCCAGGTTAAACCACGGTTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCGTGTAGTAATGGGTCACTCGTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCAGGCGGTTAACAGG‑AAGGGc < 2:284822/250‑1 (MQ=255) cGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGAtt < 1:196724/251‑1 (MQ=255) cgcgcgACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGACAGc > 1:336491/1‑251 (MQ=255) ggCATTGCGCCGGAGAAAAA‑GACCGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACCCCTCCACCGCCAGGTTAAACCACGGGACGGAAGAGCCAGAGCTGAGCAGGCGTCATGTGGCCGCAACGCTTTCCTTTCTTGTAGTAATGGGACCCTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGATTGCTGGCGGTTTACAGGGAAGGGCG‑TTGCTTCCAACggggaccttttccccttccacacg > 2:327081/1‑230 (MQ=255) cATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTg < 1:431199/251‑1 (MQ=255) ggcgcCAGAGAAACA‑GAACGCGCGGCGTGGCGGGCATCTGGCGAAAAATCCACTCCCCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCg < 2:220223/249‑1 (MQ=255) ccAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCa < 1:335622/251‑1 (MQ=255) cAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGGTTGCTGGCGGGTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTACGTTTCCCCTGTAGCAGCGTGCGGGTCAg < 1:51664/251‑1 (MQ=255) cA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACATG‑AAGGGCGCTTGCTGCCCACGGGTACGTTTACCCTGTAACAGCGCGCggggcatgactacgc > 2:29766/1‑239 (MQ=255) a‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTacgcc < 1:188386/251‑4 (MQ=255) aaCGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAgg < 1:105309/191‑1 (MQ=255) gtcGGGCATCTGGCTAAAAATACACCCCTCCACCGCCAGGGTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCTCTTTCTTCCTCTTCCGGCACCGGTGTGCTGGCGGTTAACAGG‑AATGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGTGCAGCACTACGCCGATGGCCAGTgaaa < 2:324382/249‑3 (MQ=255) | GAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGG‑AAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGA > NC_000913/4623800‑4624278 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |