Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,983	T→C	V45V (GTA→GTG)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,983	0	T	C	100.0%	195.0 / NA	58	V45V (GTA→GTG)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/33);  total (25/33)

CACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGC  >  NC_000913/4623743‑4624221                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                caccaGATCCTTCCGCCCGGACGCTTCGGCGCTGACGCCAAGCTCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgtgt                                                                                                                                                                                                                                       <  2:398187/251‑1 (MQ=255)               ccccggaccttgcGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGAACAATCGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCag                                                                                                                                                                                                                         <  2:302465/239‑1 (MQ=255)                   ggACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGaa                                                                                                                                                                                                                     <  2:274298/251‑1 (MQ=255)                                cTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCTACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGGATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCACGCGCCCAATCACTACCGTGTCGTCATTGCGCCAGAGAAACA‑Gactcgcctg                                                                                                                                                                                                       >  2:129660/1‑243 (MQ=255)                                  gACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTAGCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTg                                                                                                                                                                                                       <  1:371003/249‑1 (MQ=255)                                    cGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTTGAGATGGTTTTATCGTACTCCGTCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTg                                                                                                                                                                                                   <  1:238515/251‑1 (MQ=255)                                    cGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGCGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTg                                                                                                                                                                                                   >  1:136551/1‑251 (MQ=255)                                      ccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGc                                                                                                                                                                                                 <  1:166763/248‑1 (MQ=255)                                            gcgTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCAt                                                                                                                                                                                           <  1:280614/251‑1 (MQ=255)                                            gcgTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCAt                                                                                                                                                                                           <  1:268497/251‑1 (MQ=255)                                                          gAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATac                                                                                                                                                                             <  1:77477/251‑1 (MQ=255)                                                              acaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATacaccc                                                                                                                                                                         >  2:107087/1‑249 (MQ=255)                                                              acaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGGTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCctc                                                                                                                                                                         >  2:266737/1‑251 (MQ=255)                                                                      cgtggggatggttttatctgtccccGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCTCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTCTCTGCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGGTCTGCGCGCGACCAATCACTAACGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCCTCCTCCCCCg                                                                                                                                                                 <  2:324751/228‑1 (MQ=255)                                                                                 ttttATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAAc                                                                                                                                                      >  1:178044/1‑251 (MQ=255)                                                                                    tatcgagccacggctgtcacgacaTAAAGGTAAAGAGGGTATTGCCGAGTTGGTGGAACCGCGCGACTCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTACTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGAGACCAACCACTACCGTGTCGGCATTGCGACAGAGAAACA‑GAACGCGCTGCGTGGCGGGAATCAGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCa                                                                                                                                                    <  2:207035/228‑1 (MQ=255)                                                                                    tATCGTACCCCGCGTTGCCGGCCATAAAGGTAAAGCAGGTATGGCCGATAGTGGGGAACCCCGCTCAGCCACCGCTACCGCGCCGGGCCAGGCGGACGTTATCTTCTTCCACCCGCCGGGTATTACCCTCTTTCCACGGGTGCTGCGCGCGACCAAGCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCa                                                                                                                                                    <  2:399333/250‑1 (MQ=255)                                                                                       cggaCTCCGGCATGCCAGCCATAAAAGCAAAGAAGGTATTACCCGGATCGTGGAACAACGCGCCACCACAGCTACTGCGCCGTGCCACGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCAACCAATCACTCCCGTGCAGGCATTGCGCCAGAGAAACA‑GAACGCACTACGTGGCGGTCCTCTGGAGAAAAATACACCACTCCACCGCCAGGTTAACCCACggg                                                                                                                                                >  2:25459/4‑251 (MQ=255)                                                                                       cGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTAATGCCGAGATCGTGGAACACCGCGCCGCCACAGCAACTACGCCGGGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTATGCGCGCGACCGATCACTACCGTGTGGGCATTGAGCAAGAGAAACATCAAC‑AGCCGCGGGTCGGGCATTGGGCTAAAAAGACACTCCCCCACCGTCAGGTTAAACAACggg                                                                                                                                                >  2:54750/1‑251 (MQ=255)                                                                                        ggaCTCCGGCTTGCCAGACATAAAAGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACC‑GAACGCGCTGCGTGGCTGCCATTGGGCGAAAAATACACTCCTCCACCCCCAGTTTCAACCACGGGt                                                                                                                                               >  2:349370/3‑251 (MQ=255)                                                                                        gTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGAGTGGCGGGCATCTGGCGAAAACTACACTCCTCCACCGCCAGGTTAAACCCCgggg                                                                                                                                               >  2:48515/1‑250 (MQ=255)                                                                                          aCTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTc                                                                                                                                              <  1:295980/250‑1 (MQ=255)                                                                                                 cTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGt                                                                                                                                      >  1:238658/1‑251 (MQ=255)                                                                                                      cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAAcg                                                                                                                                                                                                            <  2:199321/176‑1 (MQ=255)                                                                                                      cagccaTAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAAcg                                                                                                                                                                                                            >  1:199321/1‑176 (MQ=255)                                                                                                             acaGGTAACGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCAAGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCTTCTGGCGAAAAATACCCTCCTCCACCGCCAGCTTACAACACGGGTCGTAAGAGTCAGAGATGAGc                                                                                                                           >  2:188445/3‑250 (MQ=255)                                                                                                                ggTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGc                                                                                                                       <  1:251912/251‑1 (MQ=255)                                                                                                                       cAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAAtgt                                                                                                                <  1:107087/251‑1 (MQ=255)                                                                                                                            aTTGCCGAGATGGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCACCCGCCGGGTATTACACTCTTTCCCCGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACa                                                                                                           <  2:395830/251‑1 (MQ=255)                                                                                                                                   gggtCGTGTAACTCCGCGCCGCCACCGCTACTGCGCCGTTCCAGGGGGACTTTATCTTCGTCCAGCCGCCGGTTATTCCACTCTTTCCACGGGTTCTGCGCGCGACCAATCGCTACCGTGTCGGAATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCGTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCATGCGTAATGTGGACAGAACGAt                                                                                                    <  2:469461/248‑1 (MQ=255)                                                                                                                                     aTCGTGGAACCCCGCGCCGCCCCCGCTCCTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGAttt                                                                                                  <  2:466244/251‑1 (MQ=255)                                                                                                                                         tGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCCTTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGGGGACATAACGATTTCCtt                                                                                              >  1:17645/1‑251 (MQ=255)                                                                                                                                          ggAACACCGCGCCGCCACCGCTACTGCGCTGTGCCAGGCGGCCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGGCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCttt                                                                                             >  1:404355/1‑251 (MQ=255)                                                                                                                                           gcacccccccccgccTCCGCTACTGCGCCGTGCCAGGCGGGCGTTATCTTCTTCCATCCGCCGGGTATTACCCTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                                                                            <  2:360170/241‑1 (MQ=255)                                                                                                                                           gAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTc                                                                                            <  1:17232/251‑1 (MQ=255)                                                                                                                                               acCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgt                                                                                        <  1:415409/251‑1 (MQ=255)                                                                                                                                                ccGCGCCGCCTCCGCTTCTGCCCCGTGCCAGGCGGACGTTATCTTCCTCCATCCGCCTGGTATTACTCTCTTTCCTCGGGTTCTGCGCGCTACCAATCACGACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACTCGCGGCGTGGCGGGCATCTGTCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgta                                                                                       <  1:227228/251‑1 (MQ=255)                                                                                                                                                 cgcgCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag                                                                                      <  1:172547/251‑1 (MQ=255)                                                                                                                                                 cgcgCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtag                                                                                      <  1:266737/251‑1 (MQ=255)                                                                                                                                                  gcgcCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                                                                                     <  1:238672/251‑1 (MQ=255)                                                                                                                                                   cgccgcCACCGCTACTGCGCCGTGCCAGGCGGACGTTATATTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGGTAAACCACGGGTCGTAAGAGTCAGGGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgtagt                                                                                     >  1:175223/1‑250 (MQ=255)                                                                                                                                                    gccgccACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTaa                                                                                   <  1:85598/251‑1 (MQ=255)                                                                                                                                                               gacggcgccgtgccAGGCGGACGTTACCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActct                                                                        <  2:178044/247‑1 (MQ=255)                                                                                                                                                                        gtgccAGGCGGACGTTATCTGCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCg                                                               <  2:78397/249‑1 (MQ=255)                                                                                                                                                                                     gTTAGCTTCTGCCATCCGCCGTGTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATTAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTATTAATGGGTCACTCTTTTTTCTCGCTTtcttcctctt                                                  <  1:221015/251‑1 (MQ=255)                                                                                                                                                                                       tATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCCCGACCACTCACTACCGTCTCGGCATTGCGCCAGAGAAACA‑GCACGCGCTGCGTGGCGGGCATACGGCGAAAAATACACCCCTCCACCGCCCGGATAAACCACGGGTCGTCAAAGTCAGAGATGGGACGGCGTAATGTGGACATAACGGTTTCCTTGCTTGTTGGAATGGGGTACTCTTTTTTCTCGCTTtcttcctcttcc                                                >  2:168960/1‑251 (MQ=255)                                                                                                                                                                                        aTCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCg                                               >  1:259360/1‑251 (MQ=255)                                                                                                                                                                                                    ccgccgGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGc                                   >  1:312610/1‑251 (MQ=255)                                                                                                                                                                                                    cagccgGGTCTTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAAAGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACACCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGAAATAAAGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCAGGCACCGGTTTGc                                   >  1:312603/3‑251 (MQ=255)                                                                                                                                                                                                     caccgGGGATTACACTCTTGCCACGGGTTCTGCGCGAGACCACTCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGATGCGTGGCGGGCCTCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGCAAGAGGCAGAGCTGGGCAGGCGTCATGAGGCCATAACGATTTCCTTTCTTGGAGATATGAGCCCCTCTTTTTTCTCGATTTCTTCCTCTTCACGCACCGGTTTGCt                                  >  2:315481/3‑251 (MQ=255)                                                                                                                                                                                                        cgGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGc                               >  1:273322/1‑251 (MQ=255)                                                                                                                                                                                                            tATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGtt                           >  1:112182/1‑251 (MQ=255)                                                                                                                                                                                                               tACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAAc                        >  2:467263/1‑251 (MQ=255)                                                                                                                                                                                                                acacTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCTGGCCTCTGGCGAAAAATCCACTCCTCCACCGCCCGGTTAACCCACGGGTCGTAATAGTCAGCGATACGCAGGCGTACTGTGATCATAACGATTTCCTTTCTTGTAGTAATGAGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGTCACCGATTTGCTGGCGGtttta                        >  2:383939/1‑247 (MQ=255)                                                                                                                                                                                                                       ttCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGTCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAgg                 <  1:200945/250‑1 (MQ=255)                                                                                                                                                                                                                         ccACGGGTGCTGCGCGAGTCCAATCTCGACCGTGGCGGCTGTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCGGGCGAAAAATTCACTCCTCCACCGCCAGGTTAAACCACGGTTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCGTGTAGTAATGGGTCACTCGTTTTTCGCGCTTTCTTCCTCTTCCGGCACCGGTTTGCAGGCGGTTAACAGGAAGGGc               <  2:284822/250‑1 (MQ=255)                                                                                                                                                                                                                            cGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGAtt           <  1:196724/251‑1 (MQ=255)                                                                                                                                                                                                                                     cgcgcgACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGACAGc  >  1:336491/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                CACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACA‑GAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGC  >  NC_000913/4623743‑4624221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑