Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,821	A→G	T99T (ACT→ACC)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,821	0	A	G	100.0%	164.6 / NA	52	T99T (ACT→ACC)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

ACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTC  >  NC_000913/4623576‑4624054                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ccggaggTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATGGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGtttt                                                                                                                                                                                                                                      <  2:288612/245‑1 (MQ=255) actgaggTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGtttt                                                                                                                                                                                                                                      <  1:8237/245‑1 (MQ=255)       gTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTa                                                                                                                                                                                                                                <  1:98538/251‑1 (MQ=255)          aTGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTc                                                                                                                                                                                                                             <  1:267688/251‑1 (MQ=255)                ttCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGc                                                                                                                                                                                                                         <  1:152508/249‑1 (MQ=255)                 tCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCtt                                                                                                                                                                                                                       <  1:423053/250‑1 (MQ=255)                    ccgccAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGGTCAGCGCCTTGAGCACAATCGACGTGGAGATGGTTTTTTCGTACTCCTGCTTgccc                                                                                                                                                                                                                   >  2:166763/1‑250 (MQ=255)                     cgccAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTgcc                                                                                                                                                                                                                    >  1:274298/1‑249 (MQ=255)                           tttCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTAGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTACGGCGGTTTTCACCCCCCGATCGATACGCCCGGCCGCTTCGGCCCTGCCGCCAAGCGCGTTCAGCGCATTGGGCACAATCGACGTGGCGAGGGTTTTATTGTACTCCGGCTTGCCAGCCAtt                                                                                                                                                                                                             >  1:291152/1‑249 (MQ=255)                                   tATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCCCCCGATCGTTACGCCCGGGCCCTTCCGCGCTGGCGCCCAGCGCGTTCCGCGCCTTGAGCCCAATCTACGGGGGGATGGTTTTATCGTACTCCGGCTTgcccaccatcaaggga                                                                                                                                                                                                       >  2:251912/1‑235 (MQ=255)                                       cGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAgg                                                                                                                                                                                                >  1:115584/1‑251 (MQ=255)                                          aTTGAGATAGTGTGCCAGGCGTCTAAGTTCGGCATTGAGCATCAAGGTGCCGTGTTGGAAGCCACGATCTTTGGTTTCGCTATATTCCGATCCTGGGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGTTCTTTACGCCCGCACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGTTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTa                                                                                                                                                                                              <  1:223295/250‑1 (MQ=255)                                            ggagaTAGTTTGCCAGGGGGCTAAGGTCGGCAGTGAGCATCAAGGGGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGGGACTTTGCGGTCGCCGTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTCCTCCGGCTTGCCAGCCATAAAGGTAAAGCCGGTATTg                                                                                                                                                                                           <  2:302782/250‑1 (MQ=255)                                              agaTAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGcc                                                                                                                                                                                         >  1:466244/1‑251 (MQ=255)                                                         cAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTgg                                                                                                                                                                               >  1:324382/1‑250 (MQ=255)                                                                ctaaGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGGGATGGTTTTATCGTACTCCGGCTTGCCAGCCATTAAGGTAAAGCAGGGATTGCCGAGATCGTGGAACACcgc                                                                                                                                                                       >  1:78397/4‑251 (MQ=255)                                                                     gTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTGCGGCGCTGACGCCAAGCGCGTTGAGCGCATTGAGCACAAACGACGTGGAGATGGttt                                                                                                                                                                                                                                       <  1:154683/182‑1 (MQ=255)                                                                               ggcaGCAAGGTGCCGTGGGGGAAGCCACGATCTTTGGTTGCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACt                                                                                                                                                        <  2:115584/248‑1 (MQ=255)                                                                                    cAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTgcgcc                                                                                                                                                   >  1:87954/1‑251 (MQ=255)                                                                                     aaGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTgcgccg                                                                                                                                                  <  1:156066/251‑1 (MQ=255)                                                                                                gtggAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGa                                                                                                                                       <  2:87954/251‑1 (MQ=255)                                                                                                  ggAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCTGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCTTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGAc                                                                                                                                      <  1:475808/250‑1 (MQ=255)                                                                                                     ggccacggtCTTGGGTTTCGGGATAGGCCGATCCTGAGCCTTTGCGGTCGCTTGCGGCGGTTTTCACCTCCGGATCGGTACGCCCGGACGCTTCGGCGCTGCCGCCAAGCGCGTGCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTa                                                                                                                                  <  2:142454/243‑1 (MQ=255)                                                                                                             tCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCCCAATCGACGGGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCCGGGATTGCCGAGATCGTGGGACACCGCGCCCCCCCCGCTACTGCGCCGTGCCAGGCGGCCGTTATCtttttc                                                                                                                          >  2:415409/1‑251 (MQ=255)                                                                                                                  gggttCGCGATAGGCCGATCCTGAGACTTTGCGGGCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATcc                                                                                                                     <  2:187473/248‑1 (MQ=255)                                                                                                                  ggTTTCGCGATAGGCCGCTCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATcc                                                                                                                     <  1:148859/251‑1 (MQ=255)                                                                                                                   gtttcgggtgaGGCCGATCCTGGGACTTTGCGGGCGCCTTCGGCGGTTGTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCTTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATccg                                                                                                                    <  2:234813/241‑1 (MQ=255)                                                                                                                   gTTTCGCGATAGGCCGATCCTGAGACTTTGAGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTAAGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGCCGTGGGGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCCGGCGGACGGTATCTTCTTCCATccc                                                                                                                    >  1:373499/1‑250 (MQ=255)                                                                                                                                  gatccTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCAACACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGGAAAGCAGGGATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGGGCCAGGCGGACGTTTTCTTCTTCCCTCCGCCGGGGATTACActc                                                                                                     >  1:424871/4‑251 (MQ=255)                                                                                                                                                  ggTCGCCTTCGGCGGTTTTCAACACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACCAACGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGGAAACCGCGCCGCCACCCCTACTGCGCCGGGCCCCGCGGACGTTATCTTCTTCCATCCGCCGGGGATTACACACTTTCCCCGGGGTCTgc                                                                                     >  2:424207/1‑251 (MQ=255)                                                                                                                                                      gCCTTCGGCGGTTTTAACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGAAGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCCGCCATAAAGGTAAAGCAGGGATTGCCGAGATCGTGGAAAACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTgcgcgc                                                                                 >  1:399119/1‑251 (MQ=255)                                                                                                                                                       ccTTCGGCGGTTTTCCCCACCAGAACGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGCCGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGGATTACACTCTTTCCCCGGGTTCTGcgcgcg                                                                                >  2:85598/1‑251 (MQ=255)                                                                                                                                                        cTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGcgcgcg                                                                                >  1:360170/1‑250 (MQ=255)                                                                                                                                                           cggcgtttttCCCCACCAGATCTGTGCGCCCGGACGATGCGGCGCGGACGCCAAGCTCGTTCAGCGCATTGGGCACAATCGACGTGGAGATGGTTTTATCGTCCCCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACTCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCa                                                                            <  2:68746/245‑1 (MQ=255)                                                                                                                                                            ggcGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGCCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGAAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCAAGGCGGCCGTTATATTCTTCCATCCGCCGGGCATTACACTCTTTCCACGGGTTCTGAGCGCGACCa                                                                            >  2:292156/3‑250 (MQ=255)                                                                                                                                                              cGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCAACGGGTTCTGCGCGCGCCCAAtc                                                                         >  1:8420/1‑250 (MQ=255)                                                                                                                                                                gTTTTCACCACCTGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCTCATTGAGCACAATCGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAAtcac                                                                       <  2:147968/251‑4 (MQ=255)                                                                                                                                                                        caccaGATCCTTCCGCCCGGACGCTTCGGCGCTGACGCCAAGCTCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgtgt                                                               <  2:398187/251‑1 (MQ=255)                                                                                                                                                                                      ccccggaccttgcGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGAACAATCGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCag                                                 <  2:302465/239‑1 (MQ=255)                                                                                                                                                                                          ggACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGaa                                             <  2:274298/251‑1 (MQ=255)                                                                                                                                                                                                       cTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCTACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGGATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCACGCGCCCAATCACTACCGTGTCGTCATTGCGCCAGAGAAACAGactcgcctg                                >  2:129660/1‑243 (MQ=255)                                                                                                                                                                                                         gACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTAGCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTg                                <  1:371003/249‑1 (MQ=255)                                                                                                                                                                                                           cGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTTGAGATGGTTTTATCGTACTCCGTCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTg                            <  1:238515/251‑1 (MQ=255)                                                                                                                                                                                                           cGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGCGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTg                            >  1:136551/1‑251 (MQ=255)                                                                                                                                                                                                             ccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGc                          <  1:166763/248‑1 (MQ=255)                                                                                                                                                                                                              caaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAacac                                                                                                                                                                          >  1:67888/3‑106 (MQ=255)                                                                                                                                                                                                              caaGCGCGCTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTGGCCAGCCATAAAGGTAAAGCAGGTATGGCCGAGATCGTGGAacac                                                                                                                                                                          <  2:67888/104‑1 (MQ=255)                                                                                                                                                                                                                   gcgTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCAt                    <  1:268497/251‑1 (MQ=255)                                                                                                                                                                                                                   gcgTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCAt                    <  1:280614/251‑1 (MQ=255)                                                                                                                                                                                                                                 gAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATac      <  1:77477/251‑1 (MQ=255)                                                                                                                                                                                                                                     acaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATacaccc  >  2:107087/1‑249 (MQ=255)                                                                                                                                                                                                                                     acaATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGGTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGCCCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACCctc  >  2:266737/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                          ACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTC  >  NC_000913/4623576‑4624054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑