Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,758	T→C	G120G (GGA→GGG)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,758	0	T	C	100.0%	132.7 / NA	47	G120G (GGA→GGG)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/24);  total (23/24)

TGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGT  >  NC_000913/4623522‑4623993                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            tGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCCGATCGTTTAGCCCGGACGCTTCGgc                                                                                                                                                                                                                               >  2:172547/1‑251 (MQ=255)             aTCCCCGGCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCCACACCAGATCGTTACGCCCGGACGCCTCCGCGCTGGCGccc                                                                                                                                                                                                                     >  2:17232/1‑248 (MQ=255)                      agcAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAg                                                                                                                                                                                                          <  1:394094/249‑1 (MQ=255)                        cAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCCGCGCTGACGCCCAGcgcggtccgcg                                                                                                                                                                                                        >  1:188910/1‑244 (MQ=255)                               gTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGCTTGGGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGATTTCAACACCAGATCGTTACGCCCGAGCGCTTCGGCGCCGACACCGAGCGCGTTACGCGCATTgtg                                                                                                                                                                                                 >  2:295980/1‑249 (MQ=255)                               gTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGTTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAg                                                                                                                                                                                                 <  1:174754/251‑1 (MQ=255)                                          cACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCGGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCACATCTTTACGCCCGGACTCGTCACCGCTGACGCCAACCGCCTTCAGCGCATTGAGCACAATCGACg                                                                                                                                                                                      >  2:172211/1‑251 (MQ=255)                                          cACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGAc                                                                                                                                                                                       <  1:351742/250‑1 (MQ=255)                                             gcgggaacgcacgggggTAATGCCTTGCGCCGCCAGTTTCTTTTTAGCCGGATGGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTGTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTgg                                                                                                                                                                                   <  2:10168/235‑1 (MQ=255)                                                       ccggaggTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATGGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGtttt                                                                                                                                                                         <  2:288612/245‑1 (MQ=255)                                                       actgaggTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGtttt                                                                                                                                                                         <  1:8237/245‑1 (MQ=255)                                                             gTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTa                                                                                                                                                                   <  1:98538/251‑1 (MQ=255)                                                                aTGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTc                                                                                                                                                                <  1:267688/251‑1 (MQ=255)                                                                      ttCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGc                                                                                                                                                            <  1:152508/249‑1 (MQ=255)                                                                       tCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCtt                                                                                                                                                          <  1:423053/250‑1 (MQ=255)                                                                          ccgccAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGGTCAGCGCCTTGAGCACAATCGACGTGGAGATGGTTTTTTCGTACTCCTGCTTgccc                                                                                                                                                      >  2:166763/1‑250 (MQ=255)                                                                           cgccAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTgcc                                                                                                                                                       >  1:274298/1‑249 (MQ=255)                                                                                 tttCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTAGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTACGGCGGTTTTCACCCCCCGATCGATACGCCCGGCCGCTTCGGCCCTGCCGCCAAGCGCGTTCAGCGCATTGGGCACAATCGACGTGGCGAGGGTTTTATTGTACTCCGGCTTGCCAGCCAtt                                                                                                                                                >  1:291152/1‑249 (MQ=255)                                                                                         tATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCCCCCGATCGTTACGCCCGGGCCCTTCCGCGCTGGCGCCCAGCGCGTTCCGCGCCTTGAGCCCAATCTACGGGGGGATGGTTTTATCGTACTCCGGCTTgcccaccatcaaggga                                                                                                                                          >  2:251912/1‑235 (MQ=255)                                                                                             cGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAgg                                                                                                                                   >  1:115584/1‑251 (MQ=255)                                                                                                aTTGAGATAGTGTGCCAGGCGTCTAAGTTCGGCATTGAGCATCAAGGTGCCGTGTTGGAAGCCACGATCTTTGGTTTCGCTATATTCCGATCCTGGGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGTTCTTTACGCCCGCACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGTTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTa                                                                                                                                 <  1:223295/250‑1 (MQ=255)                                                                                                  ggagaTAGTTTGCCAGGGGGCTAAGGTCGGCAGTGAGCATCAAGGGGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGGGACTTTGCGGTCGCCGTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTCCTCCGGCTTGCCAGCCATAAAGGTAAAGCCGGTATTg                                                                                                                              <  2:302782/250‑1 (MQ=255)                                                                                                    agaTAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGcc                                                                                                                            >  1:466244/1‑251 (MQ=255)                                                                                                               cAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTgg                                                                                                                  >  1:324382/1‑250 (MQ=255)                                                                                                                      ctaaGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGGGATGGTTTTATCGTACTCCGGCTTGCCAGCCATTAAGGTAAAGCAGGGATTGCCGAGATCGTGGAACACcgc                                                                                                          >  1:78397/4‑251 (MQ=255)                                                                                                                           gTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTGCGGCGCTGACGCCAAGCGCGTTGAGCGCATTGAGCACAAACGACGTGGAGATGGttt                                                                                                                                                                          <  1:154683/182‑1 (MQ=255)                                                                                                                                     ggcaGCAAGGTGCCGTGGGGGAAGCCACGATCTTTGGTTGCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACt                                                                                           <  2:115584/248‑1 (MQ=255)                                                                                                                                          cAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTgcgcc                                                                                      >  1:87954/1‑251 (MQ=255)                                                                                                                                           aaGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTgcgccg                                                                                     <  1:156066/251‑1 (MQ=255)                                                                                                                                                      gtggAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGa                                                                          <  2:87954/251‑1 (MQ=255)                                                                                                                                                        ggAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCTGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCTTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGAc                                                                         <  1:475808/250‑1 (MQ=255)                                                                                                                                                           ggccacggtCTTGGGTTTCGGGATAGGCCGATCCTGAGCCTTTGCGGTCGCTTGCGGCGGTTTTCACCTCCGGATCGGTACGCCCGGACGCTTCGGCGCTGCCGCCAAGCGCGTGCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTa                                                                     <  2:142454/243‑1 (MQ=255)                                                                                                                                                                   tCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCCCAATCGACGGGGAGATGGGTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCCGGGATTGCCGAGATCGTGGGACACCGCGCCCCCCCCGCTACTGCGCCGTGCCAGGCGGCCGTTATCtttttc                                                             >  2:415409/1‑251 (MQ=255)                                                                                                                                                                        gggttCGCGATAGGCCGATCCTGAGACTTTGCGGGCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATcc                                                        <  2:187473/248‑1 (MQ=255)                                                                                                                                                                        ggTTTCGCGATAGGCCGCTCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATcc                                                        <  1:148859/251‑1 (MQ=255)                                                                                                                                                                         gtttcgggtgaGGCCGATCCTGGGACTTTGCGGGCGCCTTCGGCGGTTGTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCTTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATccg                                                       <  2:234813/241‑1 (MQ=255)                                                                                                                                                                         gTTTCGCGATAGGCCGATCCTGAGACTTTGAGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTAAGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGCCGTGGGGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCCGGCGGACGGTATCTTCTTCCATccc                                                       >  1:373499/1‑250 (MQ=255)                                                                                                                                                                                        gatccTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCAACACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGGAAAGCAGGGATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGGGCCAGGCGGACGTTTTCTTCTTCCCTCCGCCGGGGATTACActc                                        >  1:424871/4‑251 (MQ=255)                                                                                                                                                                                                        ggTCGCCTTCGGCGGTTTTCAACACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACCAACGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGGAAACCGCGCCGCCACCCCTACTGCGCCGGGCCCCGCGGACGTTATCTTCTTCCATCCGCCGGGGATTACACACTTTCCCCGGGGTCTgc                        >  2:424207/1‑251 (MQ=255)                                                                                                                                                                                                            gCCTTCGGCGGTTTTAACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGAAGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCCGCCATAAAGGTAAAGCAGGGATTGCCGAGATCGTGGAAAACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTgcgcgc                    >  1:399119/1‑251 (MQ=255)                                                                                                                                                                                                             ccTTCGGCGGTTTTCCCCACCAGAACGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGCCGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGGATTACACTCTTTCCCCGGGTTCTGcgcgcg                   >  2:85598/1‑251 (MQ=255)                                                                                                                                                                                                              cTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGcgcgcg                   >  1:360170/1‑250 (MQ=255)                                                                                                                                                                                                                 cggcgtttttCCCCACCAGATCTGTGCGCCCGGACGATGCGGCGCGGACGCCAAGCTCGTTCAGCGCATTGGGCACAATCGACGTGGAGATGGTTTTATCGTCCCCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACTCCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCa               <  2:68746/245‑1 (MQ=255)                                                                                                                                                                                                                  ggcGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGCCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGAAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCAAGGCGGCCGTTATATTCTTCCATCCGCCGGGCATTACACTCTTTCCACGGGTTCTGAGCGCGACCa               >  2:292156/3‑250 (MQ=255)                                                                                                                                                                                                                    cGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCAACGGGTTCTGCGCGCGCCCAAtc            >  1:8420/1‑250 (MQ=255)                                                                                                                                                                                                                      gTTTTCACCACCTGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCTCATTGAGCACAATCGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAAtcac          <  2:147968/251‑4 (MQ=255)                                                                                                                                                                                                                              caccaGATCCTTCCGCCCGGACGCTTCGGCGCTGACGCCAAGCTCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgtgt  <  2:398187/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            TGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGT  >  NC_000913/4623522‑4623993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑