Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,410	T→C	I176I (ATT→ATC)	deoD →	purine nucleoside phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,410	0	T	C	97.6%	110.5 / NA	41	I176I (ATT→ATC)	deoD	purine nucleoside phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (21/19);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGCATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCTAAAGCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGC  >  NC_000913/4621165‑4621653                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    gTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTCGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGCTGGAAGAATACTGCATCCTCgg                                                                                                                                                                                                                                                >  2:116660/1‑251 (MQ=255)                aaaCTGCTCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGCAGCCGTTGTAAAGACCATGACTTTGCCGCTATCGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCGAAAGCGCTGGGTATTGATGCGCGCGGGGGTAACCTGTGCTCCGCTGACCTGTTCGACTCGCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGGGGAAATGGAAGc                                                                                                                                                                                                                                 <  2:49904/251‑1 (MQ=255)                   cTGCGCGACGTCGTTATCGGTATGGGAGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCACTGACCTGTTCTACTCTCCGGACGGCGAACTGTTCGCCGTGGTGGGAAAATAGCGCATCCTCGGCGGGGAAATGGATGCGGc                                                                                                                                                                                                                              >  2:198614/1‑251 (MQ=255)                            gtcgtTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGCTGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCt                                                                                                                                                                                                                      >  1:83385/1‑250 (MQ=255)                                 tATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGGGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTTTACTCTCCGGCCGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGCACTGGAAGCGGCTggggtttatggcg                                                                                                                                                                                                                >  2:356145/1‑240 (MQ=255)                                    cGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGCCCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGGATCTAAGGCGTcc                                                                                                                                                                                                             >  2:71460/1‑250 (MQ=255)                                         tGGGTGCCTGCACCGATTCCAAAGTTAACCGTATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGc                                                                                                                                                                                                         >  1:258474/1‑250 (MQ=255)                                             ggCCTTCACCGATTCCAAAGTTAACCGTATCCGGTTTAAAGACCATGACGTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGGTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCGCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCGGCAGagt                                                                                                                                                                                                    <  2:207056/250‑3 (MQ=255)                                                                     ccgtttCCGTTTTAAAGACCATGACTTTGCCGTTATCGCTGACTTTGACATGGTGCGAGCCGCTGTTGACGCGGCTAAAGCCCTGGGGTTTTATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTTGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCagct                                                                                                                                                                            <  2:360155/246‑4 (MQ=255)                                                                            cGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGt                                                                                                                                                                     >  1:354334/1‑251 (MQ=255)                                                                                     gACCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGAccc                                                                                                                                                            >  1:395023/1‑250 (MQ=255)                                                                                      aCCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTACCGCGGTAGACGCTGCTAAAGCACTGGGGGTTGATGCTCGCGTGTGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCAGCCTCGGCGTGGAAATGGAAGCGGCTGGTGGCTACCGCCTCGCTGCAGAGTTTTGCGCGAACGCCCTTACCATCTgacacgtctctgaaccc                                                                                                                                                           >  1:116767/1‑235 (MQ=255)                                                                                      aCCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAACGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTCCGGGGTCGCTGCCGAGTTTGGCGCGAAAGCCCCGACCCTCTGCCCCGTGTCTgcaccc                                                                                                                                                           >  2:247808/1‑246 (MQ=255)                                                                                      aCCATGACTTTGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGGCGCTGCGGAGTTTGGCGCGAAAGCCCTGGCCATCTGCACCGTGCCTGACcac                                                                                                                                                           >  2:133097/1‑251 (MQ=255)                                                                                              tttGCCGCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCAc                                                                                                                                                   >  1:243764/1‑251 (MQ=255)                                                                                                    gCTATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGGTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTAAGGCGTCGCTGAAGAGTTTGGCGCGAAAGCCCTTAACATCTGGACCggggcttaccccctcctccctcactg                                                                                                                                             >  2:231353/1‑226 (MQ=255)                                                                                                      tATCGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACgcgc                                                                                                                                           >  1:77924/1‑248 (MQ=255)                                                                                                       aTCGCTGACTTTGACATGGGGCGGAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCGGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGGTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGGGTCTGACCACATCCGCACTCGCGAGc                                                                                                                                           <  2:471840/250‑1 (MQ=255)                                                                                                         cGCTGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAg                                                                                                                                         <  2:326775/250‑1 (MQ=255)                                                                                                            tGACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGAccc                                                                                                                                     >  1:43323/1‑250 (MQ=255)                                                                                                             gactttgtcATGGTGCGTAACGCGGTATACGCGGCGAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCGCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTTGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCTCCGTGTCTGACCACATCCGCACTCACGAGCAGACCa                                                                                                                                     <  1:328006/242‑1 (MQ=255)                                                                                                             gACTTTGACATGGTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGGAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGGCGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTG‑TATCTACGGCGTCGCTGCAg                                                                                                                                                                                                       >  1:123652/1‑183 (MQ=255)                                                                                                                        ggTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAg                                                                                                                          <  1:152808/250‑1 (MQ=255)                                                                                                                         gTGCGTAACGCGGTAGACGCGGCTAAAGCACTGGGTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCCCTGCAGAGTTTGGCGCGAAAGCCCTGACCCTCTGCACCGTGTCTGGACACATCAGCCCTCCCGAGCAAACCACTGcccatgtgcg                                                                                                                        >  2:353498/1‑243 (MQ=255)                                                                                                                                                   aGCACTGGGTGTTGATGCTCGCGTGGGTAGCCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGCAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCCCGAGCAGACCACTGCCGCTGAGCGTCAGACAACCTTCCACGACATgctc                                                                                               >  2:31856/1‑247 (MQ=255)                                                                                                                                                         gggTGTTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCGACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGTTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAAt                                                                                          <  1:236753/249‑1 (MQ=255)                                                                                                                                                            tgttGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATc                                                                                               <  2:292354/239‑1 (MQ=255)                                                                                                                                                            tgttGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATc                                                                                               >  1:292354/3‑241 (MQ=255)                                                                                                                                                                      cgcgTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATCCGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCg                                                                            <  2:397611/250‑1 (MQ=255)                                                                                                                                                                      cgcgTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGt                                                                           <  1:136596/251‑1 (MQ=255)                                                                                                                                                                      cgcgTGGGTAACCGGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGt                                                                           <  2:77924/251‑1 (MQ=255)                                                                                                                                                                                ccctggtcgccGCTGATCTGTTCTACTCTCCGGACGGCGAAATGGTCGACGGGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGTCCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCCTCTGGGc                                                                 <  2:98157/242‑1 (MQ=255)                                                                                                                                                                                              gACCTGGTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAAGACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTAGCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCACTGACCATCTGCACCGTGTCTGACCACATCCGCACCCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTGCAACGACATGAGCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAAtt                                                   <  1:216769/251‑1 (MQ=255)                                                                                                                                                                                                    ttCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGttt                                             >  1:378505/1‑251 (MQ=255)                                                                                                                                                                                                         cgcgacGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCATGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTg                                        <  1:198614/246‑1 (MQ=255)                                                                                                                                                                                                                    gCGAAATGTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTTGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGaacg                             <  1:231353/251‑4 (MQ=255)                                                                                                                                                                                                                      gAAATGTTCGACGTGATTGAAAAATACGGCATCCGCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGaacgtc                           <  2:288366/251‑6 (MQ=255)                                                                                                                                                                                                                           gTTCGACGTGATGGAAAAATACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGaacgtcgtgt                       <  1:100621/250‑10 (MQ=255)                                                                                                                                                                                                                               gACGTGATGGAAAAATGCGGCCTCCGCGGCGTGGAAAGGGAATCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGaacgtcgtgttaagc                  <  2:255565/251‑15 (MQ=255)                                                                                                                                                                                                                                             aTACGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGTCGTGTTAAGCCGGAGCAGGAGACt    <  1:378675/251‑1 (MQ=255)                                                                                                                                                                                                                                               aCGGCATCCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAGTTTGGCGCGAAAGCCCTGACCATCTGCACCGTGTCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACCACCTTCAACGACATGATCAAAATCGCCCTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGAAAGGCGAACGGCGTGTTAATCCGGAGCAGGAGACTgt  >  2:379994/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GTTCTGCCGCACGTAAAACTGCGCGACGTCGTTATCGGTATGGGTGCCTGCACCGATTCCAAAGTTAACCGCATCCGTTTTAAAGACCATGACTTTGCCGCTATCGCTGACTTCGACATGGTGCGTAACGCAGTAGATGCAGCTAAAGCACTGGGTATTGATGCTCGCGTGGGTAACCTGTTCTCCGCTGACCTGTTCTACTCTCCGGACGGCGAAATGTTCGACGTGATGGAAAAATACGGCATTCTCGGCGTGGAAATGGAAGCGGCTGGTATCTACGGCGTCGCTGCAGAATTTGGCGCGAAAGCCCTGACCATCTGCACCGTATCTGACCACATCCGCACTCACGAGCAGACCACTGCCGCTGAGCGTCAGACTACCTTCAACGACATGATCAAAATCGCACTGGAATCCGTTCTGCTGGGCGATAAAGAGTAATTGTGTTTCGCTGCAAGGCGATTGCCTTGTGAAGCCGGAGCGGGAGACTGC  >  NC_000913/4621165‑4621653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑