Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,652	C→T	C350C (TGC→TGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,652	0	C	T	98.4%	193.7 / ‑4.5	62	C350C (TGC→TGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (20/41);  total (20/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

CACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAA  >  NC_000913/4620409‑4620878                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   cACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCg                                                                                                                                                                                                                              <  2:373871/250‑1 (MQ=255)  aCCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGAc                                                                                                                                                                                                                            <  1:73568/251‑1 (MQ=255)              aaaGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTAc                                                                                                                                                                                                                >  1:295513/1‑251 (MQ=255)                  caaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAc                                                                                                                                                                                                            >  1:79757/3‑251 (MQ=255)                  caaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAc                                                                                                                                                                                                            <  1:258683/249‑1 (MQ=255)                   aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                                                                                           >  1:461848/2‑251 (MQ=255)                    aCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACca                                                                                                                                                                                                          <  1:134740/251‑1 (MQ=255)                    aCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACca                                                                                                                                                                                                          <  1:155146/251‑1 (MQ=255)                    aCTGGCCTGGACGCGCTGTTGGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACGTCGGTGACTTCGACTCTTCCGGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACca                                                                                                                                                                                                          <  2:86622/251‑1 (MQ=255)                       ggCCTGGACGCGCGGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACcaca                                                                                                                                                                                                        <  1:425158/250‑1 (MQ=255)                       ggCCGGGACGCGCTGTTTGACGCCCCCATCAAAGAGATGAAAGAAGCGGGTTATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTGCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACcac                                                                                                                                                                                                         <  2:351695/249‑1 (MQ=255)                         ccgggACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCGTCACCAACGTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACAcgc                                                                                                                                                                                                     <  1:154931/248‑1 (MQ=255)                          cTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACCcgcg                                                                                                                                                                                                    >  2:400969/1‑251 (MQ=255)                                   cTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACAt                                                                                                                                                                                           >  1:291553/1‑251 (MQ=255)                                          ccGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTCCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTa                                                                                                                                                                                    <  2:130684/250‑1 (MQ=255)                                          aCGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGt                                                                                                                                                                                     >  1:177868/1‑250 (MQ=255)                                          aCGCCACCAGCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCTCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGTCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTTATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTa                                                                                                                                                                                    <  1:292158/251‑1 (MQ=255)                                            gCCACCATCAAAGAGATGAAAGAATCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACt                                                                                                                                                                                  <  1:457614/251‑1 (MQ=255)                                               accaTCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                                                                                               <  1:406045/251‑1 (MQ=255)                                                    cAAAGAGATGAAAGAAGCGGGTGATAACACCATCGGCTTCACCAACTTCGTTGACTTCGACTCTTCCGGGGGCCACCGGCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacg                                                                                                                                                                          <  2:111492/251‑6 (MQ=255)                                                    aaaaGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttac                                                                                                                                                                           <  2:241862/249‑5 (MQ=255)                                                       agagATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGcc                                                                                                                                                                       <  1:400969/251‑1 (MQ=255)                                                         agaTGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGGCTCTCCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCg                                                                                                                                                                     <  2:174773/251‑1 (MQ=255)                                                            ggAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACGTCGTTGACTGCGACGCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGaaa                                                                                                                                                                  <  2:461848/250‑1 (MQ=255)                                                                   agCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGGCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAc                                                                                                                                                           <  1:316473/251‑1 (MQ=255)                                                                    gCGGGTGATAACACCATCGTCTCCACCAACTTCGGTGACTGCGACTCTTCCGGGGGCCACCGCCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGCCCACTGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAcc                                                                                                                                                          <  2:33991/251‑1 (MQ=255)                                                                        gTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGc                                                                                                                                                      <  1:113761/251‑1 (MQ=255)                                                                               cacCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGTCTGCCGGAGCTGTTGTCTCTGCTGCGCGCTGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGGACTGGTTTACGGCCAGaacagaaaacccgggacactgg                                                                                                                                               >  2:16292/1‑231 (MQ=255)                                                                                acCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTggg                                                                                                                                              <  1:98955/251‑1 (MQ=255)                                                                                  cATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTTGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTAACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGAAAAACCGGGCTCACTGGGTc                                                                                                                                            >  1:46373/1‑251 (MQ=255)                                                                                      gTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCaccg                                                                                                                                        >  2:425363/1‑248 (MQ=255)                                                                                         ttCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGCTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGGAAAACCGGGCTCACTGGGTCaccggg                                                                                                                                      >  1:293517/1‑245 (MQ=255)                                                                                          tCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTg                                                                                                                                      <  2:492654/249‑1 (MQ=255)                                                                                           cACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGGTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGaa                                                                                                                                    <  1:441806/250‑1 (MQ=255)                                                                                           cACCAACTTCGTTGACTTCGACTCTTACTGGGGCCACCGTCACGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCaccgcgaa                                                                                                                                    >  1:388355/1‑243 (MQ=255)                                                                                             ccAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAAcc                                                                                                                                 >  1:241578/1‑251 (MQ=255)                                                                                                    cGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTACAACCGGGCTCACTGGGTCACCGTGAAACCTTCCCgg                                                                                                                          >  2:325779/1‑251 (MQ=255)                                                                                                     gTTGACTTCGCCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTCTGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGTGCTCCGACCTGGACCGGTACTGACCACACGCGTGAACACCTTCCGGTACTGGTTTACGGCCCGAAAGTCAAACCGGGCTCACTGGGTCACCAGAAAACCTTCGCggg                                                                                                                         >  2:203636/1‑250 (MQ=255)                                                                                                      ttGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGAAAAACCGGGCACCCTGGGGCACCGTGAAACCTTCGCgg                                                                                                                          >  2:269262/1‑249 (MQ=255)                                                                                                        gACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGatat                                                                                                                      >  1:408832/1‑251 (MQ=255)                                                                                                                    tCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct                                                                                                            <  1:157239/249‑1 (MQ=255)                                                                                                                    tCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAc                                                                                                             <  1:192085/248‑1 (MQ=255)                                                                                                                    tCCTGGGGCCACCGGCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGAct                                                                                                            <  1:348502/249‑1 (MQ=255)                                                                                                                     ccTGGGGCCCCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTg                                                                                                         <  1:67650/251‑1 (MQ=255)                                                                                                                              acagTCGCGACGTCGCCGTGTATGCGGCGGTTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCTCACGCGTGAACACATTCCGGTACTTGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCGGGCAAAatat                                                                                                <  2:67907/248‑1 (MQ=255)                                                                                                                               ccGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGTTCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGTAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATAtt                                                                                               <  1:220221/251‑1 (MQ=255)                                                                                                                                cGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATAttt                                                                                              <  1:183883/251‑1 (MQ=255)                                                                                                                                    gcgACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGt                                                                                          <  1:314170/251‑1 (MQ=255)                                                                                                                                     ggACGTCGCCGGTTATGTTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCGCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGGTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTa                                                                                         <  2:370290/250‑1 (MQ=255)                                                                                                                                     cgACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTa                                                                                         <  1:305268/251‑1 (MQ=255)                                                                                                                                           cGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTTACCACGGTTGTGATCCGACCTGGACCGGTACGGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTg                                                                                   <  2:447211/251‑1 (MQ=255)                                                                                                                                           cGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGCAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTg                                                                                   >  1:474111/1‑251 (MQ=255)                                                                                                                                                   aTGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGaa                                                                           <  1:425363/251‑1 (MQ=255)                                                                                                                                                               tGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGcc                                                               >  1:215990/1‑251 (MQ=255)                                                                                                                                                                      gTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCt                                                        <  1:393229/251‑1 (MQ=255)                                                                                                                                                                                cgTCTTCCGGAGATGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCGCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggt                                              <  1:3585‑M1/251‑8 (MQ=255)                                                                                                                                                                                      ccGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggct                                        >  1:473710‑M1/1‑238 (MQ=255)                                                                                                                                                                                       cGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggctt                                       <  2:291553‑M1/251‑15 (MQ=255)                                                                                                                                                                                                              gATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggcccccgc                <  2:163271‑M1/251‑38 (MQ=255)                                                                                                                                                                                                                      cATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACaaaaa        <  2:145314/251‑1 (MQ=255)                                                                                                                                                                                                                       aTCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgcccccaccccggccccctcccacagggg       >  2:135154‑M1/1‑205 (MQ=255)                                                                                                                                                                                                                            gttccTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGGATATTGGTCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATaatgggtgcggcttgatgccctcaccccggccctctcccacagggagaggg  <  1:493516‑M1/249‑52 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   CACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAA  >  NC_000913/4620409‑4620878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑