| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 5,099 | T→C | intergenic (+79/‑135) | thrC → / → yaaX | threonine synthase/DUF2502 domain‑containing protein YaaX |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 5,099 | 0 | T | C | 89.3% | 67.6 / 1.0 | 29 | intergenic (+79/‑135) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein YaaX |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (13/12); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-02 | |||||||||||
CGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAAT > NC_000913/4855‑5333 | cGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAggg > 1:294053/1‑251 (MQ=255) ccGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGaaaa > 1:444036/1‑250 (MQ=255) cgcacatgcGGCGAAATTAAAAGAGAGCGTGGAAGCGATTCCCGTTGAAACTT‑TGGATCTGCAAAAAGAGCTGGCAGATCGTGCTGATTTACCCTTGCTTTACCATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAg < 2:444036/243‑1 (MQ=255) aTTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAAt > 1:135412/1‑250 (MQ=255) taaaaagcgtgTGGAATCGATTCTCGGT‑AAACCTATGGATCTGCAAAAAGAGCTGTCAGATCGGGCGTATTAACCATCTCTTTCACATAA‑CTCCCCCGCCTATTTTGCTTCTGTGCGTAAATTGATGATGAATCATCTGTAAAATCTATTCATTATCTCAATCAGGCCGGATTTGCTTTTATGCAGCT‑TGCTTTTTTATTAAGAAATGATGGAGAATAACGACAGGGAAATAGTATAAAGTCACAATaaa < 2:74499/240‑1 (MQ=255) gATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGAtt > 2:437126/1‑250 (MQ=255) attctcgggtaataagtggtaTCTACCAAAAGAGCGGGCAGAACTGGCTGATTGCCCTTTGTTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCTTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCGTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTACCTTAGAGATTa < 2:499323/231‑1 (MQ=255) tGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCAGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCgg > 1:50563/1‑251 (MQ=255) tGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGAAAAATCTATTCATTATATCAATCAGGCCGAGTTTGATTTTATGCAGCC‑GGCTTTTTTATAAAGAAATTATGGAGAAAAACGACAGGGAAACAGGAGAAATTCTCAATAACTGCGGTAACTTAGAGATTAGGATTGCgg > 1:286578/1‑251 (MQ=255) aacgtgggaacggCCAAAAGAGTGGGCAGAACGGTCTGATTTCCCTTTGCTTTCACATATTCGGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGgaga < 1:22939/239‑1 (MQ=255) gATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATaa > 1:252684/1‑213 (MQ=255) tCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAAcc > 1:168474/1‑251 (MQ=255) aaaaaGAGCTGGCAGAACGTGTTTATTTACCCTTGCTTTAACATAATCTGCCC‑GCCGATTTTGCTGCGGTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACcgtcgt < 1:373106/250‑1 (MQ=255) aaatagcgggCAGACCGTGCTGACTTCCCCTTTCTTACATATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTtc < 2:447430/243‑1 (MQ=255) agCTGGCAGCACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACCGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTACGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCAt > 2:311122/1‑251 (MQ=255) tggattctattaatgcttccc‑GCCGATTTTGCTGTGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGACGGGTTTGCTTTTATGCAGCC‑GGCTTGTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCa < 1:45265/232‑1 (MQ=255) gCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGAAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATa > 1:347978/1‑250 (MQ=255) cctccgATGTTGCTGCGTTGCGTAAAATGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCA‑TCCGGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGCTaaacgg < 2:96154/248‑4 (MQ=255) cgATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGt > 2:363565/1‑250 (MQ=255) gatgaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAAAGACAGGGAAAAAGGAGACATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGACGATCTCATCGCGTAATCTCCGGATATCGAACCATAACGGGCAATGATAAAAGGAGGAACCTATGAAAAAGATGCAATCTAt > 1:346194/1‑248 (MQ=255) gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAAt < 2:210404/112‑1 (MQ=255) gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAAt > 1:210404/1‑112 (MQ=255) gaATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTAc < 1:434251/250‑1 (MQ=255) aTTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGTAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGt < 2:294053/248‑1 (MQ=255) gCCGGGTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGGGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGAGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCAATAACGGGCAATGATAAAAGGAGTAGCCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCACATGgcagc > 1:1871/1‑251 (MQ=255) ggTTTGCTTTTATGCAGCC‑GGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGcac > 2:74773/1‑249 (MQ=255) ccGGCTTTTTTATGAAGAAATTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGtt < 2:79790/251‑1 (MQ=255) aagaaATGATGGAGAAAAACGGCAGGGAAAAAGGAGAAAATCGCAATAAATGCGGTAACGTAGAGATTAGGAGGGGGGAGAATAACAGCCGTCGTTCTCATCGGGTAATCGCCGGATAGCGACCCATAACGGGCAATGATAAAAGGAGTACCCTATGAAAAAGATGCAATCTATCGTACTCGCACTGGCCCTGGTTCTGGGCGCGGCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTaa < 2:48816/251‑1 (MQ=255) aaaTTATGGAGAAAAACGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGTCGTTCTCATCGCGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTATGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGACGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAAt > 1:504209/1‑251 (MQ=255) | CGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGT‑TGGATCTGCCAAAAGAGCTGGCAGAACGTGCTGATTTACCCTTGCTTTCACATAATCTGCCC‑GCCGATTTTGCTGCGTTGCGTAAATTGATGATGAATCATCAGTAAAATCTATTCATTATCTCAATCAGGCCGGGTTTGCTTTTATGCAGCCCGGCTTTTTTATGAAGAAATTATGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATGCAATCTATCGTACTCGCACTTTCCCTGGTTCTGGTCGCTCCCATGGCAGCACAGGCTGCGGAAATTACGTTAGTCCCGTCAGTAAAAT > NC_000913/4855‑5333 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |